A system for the directed evolution of tetracyclines

Louis Henri Scott

A system for the directed evolution of tetracyclines

Louis Henri Scott

Research output: Thesis › Doctoral Thesis

167 Downloads (Pure)

Abstract

Therise of antimicrobial resistance is a major threat to global health that requires action across all government sectors and society. Without new methods for antibiotic discovery, people will die from historically easy to treat infections. This work outlines a new genetic engineering approach that harnesses the power of synthetic biology for the directed evolution of antibiotics in the yeast Saccharomyces cerevisiae. The strength of this platform lies in the use of an evolutionary driving force that selects for the biosynthesis of antibiotics that can overcome bacterial resistance. This system presents a significant addition to the antibiotic discovery toolbox.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Filipovska, Aleksandra, Supervisor Rackham, Oliver, Supervisor Waterer, Grant, Supervisor
Award date	7 Oct 2016
Publication status	Unpublished - 2016

Access to Document

THESIS - DOCTOR OF PHILOSOPHY - SCOTT Louis Henri - 2016
This work is protected by Copyright. You may print or download ONE copy of this document for the purpose of your own non-commercial research or study. Any other use requires permission from the copyright owner. The Copyright Act requires you to attribute any copyright works you quote or paraphrase.

Cite this

@phdthesis{eb97f635ca4a496eaff4ab1bf5a43ade,

title = "A system for the directed evolution of tetracyclines",

abstract = "Therise of antimicrobial resistance is a major threat to global health that requires action across all government sectors and society. Without new methods for antibiotic discovery, people will die from historically easy to treat infections. This work outlines a new genetic engineering approach that harnesses the power of synthetic biology for the directed evolution of antibiotics in the yeast Saccharomyces cerevisiae. The strength of this platform lies in the use of an evolutionary driving force that selects for the biosynthesis of antibiotics that can overcome bacterial resistance. This system presents a significant addition to the antibiotic discovery toolbox.",

keywords = "Direct evolution, Polyketide, Synthetic biology, Antibiotic, Tetracycline, Oxytetracline, Genetic selection, Biosynthetic pathway",

author = "Scott, {Louis Henri}",

year = "2016",

language = "English",

school = "The University of Western Australia",

}

TY - THES

T1 - A system for the directed evolution of tetracyclines

AU - Scott, Louis Henri

PY - 2016

Y1 - 2016

N2 - Therise of antimicrobial resistance is a major threat to global health that requires action across all government sectors and society. Without new methods for antibiotic discovery, people will die from historically easy to treat infections. This work outlines a new genetic engineering approach that harnesses the power of synthetic biology for the directed evolution of antibiotics in the yeast Saccharomyces cerevisiae. The strength of this platform lies in the use of an evolutionary driving force that selects for the biosynthesis of antibiotics that can overcome bacterial resistance. This system presents a significant addition to the antibiotic discovery toolbox.

AB - Therise of antimicrobial resistance is a major threat to global health that requires action across all government sectors and society. Without new methods for antibiotic discovery, people will die from historically easy to treat infections. This work outlines a new genetic engineering approach that harnesses the power of synthetic biology for the directed evolution of antibiotics in the yeast Saccharomyces cerevisiae. The strength of this platform lies in the use of an evolutionary driving force that selects for the biosynthesis of antibiotics that can overcome bacterial resistance. This system presents a significant addition to the antibiotic discovery toolbox.

KW - Direct evolution

KW - Polyketide

KW - Synthetic biology

KW - Antibiotic

KW - Tetracycline

KW - Oxytetracline

KW - Genetic selection

KW - Biosynthetic pathway

M3 - Doctoral Thesis

ER -

A system for the directed evolution of tetracyclines

Abstract

Access to Document

Fingerprint

Cite this