A study on the expression profile and redox potency of carcinogenic thioredoxin from human lung adenocarcinoma epithelial cells

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Abstract

The aim of this study was to explore the expression pattern and redox potency of the mortality combating thioredoxin isolated from human adenocarcinoma cells of lung and this could not only serve as potential new diagnostic/predictive marker but also aid in targeted therapy. The expression profile of thioredoxin (Trx) in human lung adenocarcinoma epithelial cells (A549) and normal lung cancer cells wasstudied by RT PCR and recombinant cloning techniques. The expression profile was analysed by Agarose and SDS PAGE electrophoretic analysis. The gene sequence was confirmed by DNA sequencing and the protein by western blot using anti-thioredoxin antibody. The REDOX activity of the thioredoxins wastested and compared by insulin reduction assay. There was upregulated and widespread expression ofthioredoxinin adenocarcinomaof lung and this protein was more potent when compared with normal physiological thioredoxin protein. There was enhanced and exaggerated dithiol-disulphideoxido reductase catalytic activity by the malignant thioredoxin which was observed as increasing reduction of insulin disulphides by dithiothreitol that could be visualized spectrophotometrically at 650 nm (25°C) as turbidity formation resulting from the precipitation of the free insulin β chain
Original languageEnglish
Pages (from-to)342-348
JournalResearch Journal of Pharmaceutical, Biological and Chemical Sciences (RJPBCS)
Volume5
Issue number3
Publication statusPublished - 2014
Externally publishedYes

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