1. 1. Neonatal rat liver slices incorporate 3H-thymidine into DNA in vitro forty times faster than adult slices. 2. 2. Glucagon (75 μg/ml) stimulates incorporation rate by 47 per cent (mean); other hormones, substrates and cylic AMP have no effect. 3. 3. No stimulation is seen with adult, regenerating or 5-day postnatal slices. 4. 4. Continuous exposure of slices to glucagon is necessary for the effect. 5. 5. In vivo administration of glucagon does not stimulate incorporation in liver slices in vitro. 6. 6. Glucagon stimulation is abolished by puromycin (10-5 M) or actinomycin D (10μg/ml) with no effect on controls. 7. 7. Haematopoeitic cells may be the target of glucagon.
|Number of pages||11|
|Journal||Comparative Biochemistry and Physiology -- Part A: Physiology|
|Publication status||Published - 1 Aug 1971|