A stimulatory effect of glucagon on DNA synthesis in neonatal rat liver

G. Yeoh; I. T. Oliver

doi:10.1016/0300-9629(71)90195-2

A stimulatory effect of glucagon on DNA synthesis in neonatal rat liver

G. Yeoh, I. T. Oliver

UWA Medical School

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

1. 1. Neonatal rat liver slices incorporate ³H-thymidine into DNA in vitro forty times faster than adult slices. 2. 2. Glucagon (75 μg/ml) stimulates incorporation rate by 47 per cent (mean); other hormones, substrates and cylic AMP have no effect. 3. 3. No stimulation is seen with adult, regenerating or 5-day postnatal slices. 4. 4. Continuous exposure of slices to glucagon is necessary for the effect. 5. 5. In vivo administration of glucagon does not stimulate incorporation in liver slices in vitro. 6. 6. Glucagon stimulation is abolished by puromycin (10-⁵ M) or actinomycin D (10μg/ml) with no effect on controls. 7. 7. Haematopoeitic cells may be the target of glucagon.

Original language	English
Pages (from-to)	723-733
Number of pages	11
Journal	Comparative Biochemistry and Physiology -- Part A: Physiology
Volume	39
Issue number	4
DOIs	https://doi.org/10.1016/0300-9629(71)90195-2
Publication status	Published - 1 Aug 1971

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title = "A stimulatory effect of glucagon on DNA synthesis in neonatal rat liver",

abstract = "1. 1. Neonatal rat liver slices incorporate 3H-thymidine into DNA in vitro forty times faster than adult slices. 2. 2. Glucagon (75 μg/ml) stimulates incorporation rate by 47 per cent (mean); other hormones, substrates and cylic AMP have no effect. 3. 3. No stimulation is seen with adult, regenerating or 5-day postnatal slices. 4. 4. Continuous exposure of slices to glucagon is necessary for the effect. 5. 5. In vivo administration of glucagon does not stimulate incorporation in liver slices in vitro. 6. 6. Glucagon stimulation is abolished by puromycin (10-5 M) or actinomycin D (10μg/ml) with no effect on controls. 7. 7. Haematopoeitic cells may be the target of glucagon.",

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AU - Oliver, I. T.

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N2 - 1. 1. Neonatal rat liver slices incorporate 3H-thymidine into DNA in vitro forty times faster than adult slices. 2. 2. Glucagon (75 μg/ml) stimulates incorporation rate by 47 per cent (mean); other hormones, substrates and cylic AMP have no effect. 3. 3. No stimulation is seen with adult, regenerating or 5-day postnatal slices. 4. 4. Continuous exposure of slices to glucagon is necessary for the effect. 5. 5. In vivo administration of glucagon does not stimulate incorporation in liver slices in vitro. 6. 6. Glucagon stimulation is abolished by puromycin (10-5 M) or actinomycin D (10μg/ml) with no effect on controls. 7. 7. Haematopoeitic cells may be the target of glucagon.

AB - 1. 1. Neonatal rat liver slices incorporate 3H-thymidine into DNA in vitro forty times faster than adult slices. 2. 2. Glucagon (75 μg/ml) stimulates incorporation rate by 47 per cent (mean); other hormones, substrates and cylic AMP have no effect. 3. 3. No stimulation is seen with adult, regenerating or 5-day postnatal slices. 4. 4. Continuous exposure of slices to glucagon is necessary for the effect. 5. 5. In vivo administration of glucagon does not stimulate incorporation in liver slices in vitro. 6. 6. Glucagon stimulation is abolished by puromycin (10-5 M) or actinomycin D (10μg/ml) with no effect on controls. 7. 7. Haematopoeitic cells may be the target of glucagon.

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KW - Glucagon stimulation

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A stimulatory effect of glucagon on DNA synthesis in neonatal rat liver

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