A standarized protocol for evaluation of large extracellular vesicles using the attune™ NXT system

Leslie Marisol Lugo-Gavidia; Dylan Burger; Erika Bosio; Sandi Robinson; Janis Nolde; Revathy Carnagarin; Vance B. Matthews; Markus P. Schlaich

doi:10.1016/j.jim.2021.113170

A standarized protocol for evaluation of large extracellular vesicles using the attune™ NXT system

Leslie Marisol Lugo-Gavidia, Dylan Burger, Erika Bosio, Sandi Robinson, Janis Nolde, Revathy Carnagarin, Vance B. Matthews, Markus P. Schlaich

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Extracellular vesicles (EVs) represent a promising biomarker in several medical areas. Flow cytometry (FC) is one of the most widely-used methods to characterize EVs, providing quantitative information and determination of EV subtypes. EV evaluation represents a challenge as no standardized methods are available to facilitate assessment across different research centers. This is principally because their size falls below the detection limit of most standard flow cytometers and a thorough optimization process is required to ensure instrument-specific sensitivity. We provide an overview of a standardized method to evaluate large EVs using the Attune™ Nxt Acoustic Focusing Flow Cytometer system (Thermo Fisher Scientific).

Original language	English
Pages (from-to)	113170
Number of pages	1
Journal	Journal of Immunological Methods
Volume	499
DOIs	https://doi.org/10.1016/j.jim.2021.113170
Publication status	Published - 1 Dec 2021

Access to Document

10.1016/j.jim.2021.113170

Cite this

@article{f750d8cf45d24319a2b38cb3fc1c191f,

title = "A standarized protocol for evaluation of large extracellular vesicles using the attune{\texttrademark} NXT system",

abstract = "Extracellular vesicles (EVs) represent a promising biomarker in several medical areas. Flow cytometry (FC) is one of the most widely-used methods to characterize EVs, providing quantitative information and determination of EV subtypes. EV evaluation represents a challenge as no standardized methods are available to facilitate assessment across different research centers. This is principally because their size falls below the detection limit of most standard flow cytometers and a thorough optimization process is required to ensure instrument-specific sensitivity. We provide an overview of a standardized method to evaluate large EVs using the Attune{\texttrademark} Nxt Acoustic Focusing Flow Cytometer system (Thermo Fisher Scientific).",

keywords = "Attune Nxt, Extracellular vesicles, Flow cytometry",

author = "Lugo-Gavidia, {Leslie Marisol} and Dylan Burger and Erika Bosio and Sandi Robinson and Janis Nolde and Revathy Carnagarin and Matthews, {Vance B.} and Schlaich, {Markus P.}",

year = "2021",

month = dec,

day = "1",

doi = "10.1016/j.jim.2021.113170",

language = "English",

volume = "499",

pages = "113170",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

}

TY - JOUR

T1 - A standarized protocol for evaluation of large extracellular vesicles using the attune™ NXT system

AU - Lugo-Gavidia, Leslie Marisol

AU - Burger, Dylan

AU - Bosio, Erika

AU - Robinson, Sandi

AU - Nolde, Janis

AU - Carnagarin, Revathy

AU - Matthews, Vance B.

AU - Schlaich, Markus P.

PY - 2021/12/1

Y1 - 2021/12/1

N2 - Extracellular vesicles (EVs) represent a promising biomarker in several medical areas. Flow cytometry (FC) is one of the most widely-used methods to characterize EVs, providing quantitative information and determination of EV subtypes. EV evaluation represents a challenge as no standardized methods are available to facilitate assessment across different research centers. This is principally because their size falls below the detection limit of most standard flow cytometers and a thorough optimization process is required to ensure instrument-specific sensitivity. We provide an overview of a standardized method to evaluate large EVs using the Attune™ Nxt Acoustic Focusing Flow Cytometer system (Thermo Fisher Scientific).

AB - Extracellular vesicles (EVs) represent a promising biomarker in several medical areas. Flow cytometry (FC) is one of the most widely-used methods to characterize EVs, providing quantitative information and determination of EV subtypes. EV evaluation represents a challenge as no standardized methods are available to facilitate assessment across different research centers. This is principally because their size falls below the detection limit of most standard flow cytometers and a thorough optimization process is required to ensure instrument-specific sensitivity. We provide an overview of a standardized method to evaluate large EVs using the Attune™ Nxt Acoustic Focusing Flow Cytometer system (Thermo Fisher Scientific).

KW - Attune Nxt

KW - Extracellular vesicles

KW - Flow cytometry

UR - http://www.scopus.com/inward/record.url?scp=85122111586&partnerID=8YFLogxK

U2 - 10.1016/j.jim.2021.113170

DO - 10.1016/j.jim.2021.113170

M3 - Article

C2 - 34662560

AN - SCOPUS:85122111586

VL - 499

SP - 113170

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

ER -

A standarized protocol for evaluation of large extracellular vesicles using the attune™ NXT system

Abstract

Access to Document

Other files and links

Fingerprint

Cite this