A standarized protocol for evaluation of large extracellular vesicles using the attune™ NXT system

Leslie Marisol Lugo-Gavidia; Dylan Burger; Erika Bosio; Sandi Robinson; Janis Nolde; Revathy Carnagarin; Vance B. Matthews; Markus P. Schlaich

doi:10.1016/j.jim.2021.113170

A standarized protocol for evaluation of large extracellular vesicles using the attune™ NXT system

Leslie Marisol Lugo-Gavidia, Dylan Burger, Erika Bosio, Sandi Robinson, Janis Nolde, Revathy Carnagarin, Vance B. Matthews, Markus P. Schlaich

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Extracellular vesicles (EVs) represent a promising biomarker in several medical areas. Flow cytometry (FC) is one of the most widely-used methods to characterize EVs, providing quantitative information and determination of EV subtypes. EV evaluation represents a challenge as no standardized methods are available to facilitate assessment across different research centers. This is principally because their size falls below the detection limit of most standard flow cytometers and a thorough optimization process is required to ensure instrument-specific sensitivity. We provide an overview of a standardized method to evaluate large EVs using the Attune™ Nxt Acoustic Focusing Flow Cytometer system (Thermo Fisher Scientific).

Original language	English
Article number	113170
Pages (from-to)	113170
Number of pages	1
Journal	Journal of Immunological Methods
Volume	499
DOIs	https://doi.org/10.1016/j.jim.2021.113170
Publication status	Published - 1 Dec 2021

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10.1016/j.jim.2021.113170

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title = "A standarized protocol for evaluation of large extracellular vesicles using the attune{\texttrademark} NXT system",

abstract = "Extracellular vesicles (EVs) represent a promising biomarker in several medical areas. Flow cytometry (FC) is one of the most widely-used methods to characterize EVs, providing quantitative information and determination of EV subtypes. EV evaluation represents a challenge as no standardized methods are available to facilitate assessment across different research centers. This is principally because their size falls below the detection limit of most standard flow cytometers and a thorough optimization process is required to ensure instrument-specific sensitivity. We provide an overview of a standardized method to evaluate large EVs using the Attune{\texttrademark} Nxt Acoustic Focusing Flow Cytometer system (Thermo Fisher Scientific).",

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AU - Lugo-Gavidia, Leslie Marisol

AU - Burger, Dylan

AU - Bosio, Erika

AU - Robinson, Sandi

AU - Nolde, Janis

AU - Carnagarin, Revathy

AU - Matthews, Vance B.

AU - Schlaich, Markus P.

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N2 - Extracellular vesicles (EVs) represent a promising biomarker in several medical areas. Flow cytometry (FC) is one of the most widely-used methods to characterize EVs, providing quantitative information and determination of EV subtypes. EV evaluation represents a challenge as no standardized methods are available to facilitate assessment across different research centers. This is principally because their size falls below the detection limit of most standard flow cytometers and a thorough optimization process is required to ensure instrument-specific sensitivity. We provide an overview of a standardized method to evaluate large EVs using the Attune™ Nxt Acoustic Focusing Flow Cytometer system (Thermo Fisher Scientific).

AB - Extracellular vesicles (EVs) represent a promising biomarker in several medical areas. Flow cytometry (FC) is one of the most widely-used methods to characterize EVs, providing quantitative information and determination of EV subtypes. EV evaluation represents a challenge as no standardized methods are available to facilitate assessment across different research centers. This is principally because their size falls below the detection limit of most standard flow cytometers and a thorough optimization process is required to ensure instrument-specific sensitivity. We provide an overview of a standardized method to evaluate large EVs using the Attune™ Nxt Acoustic Focusing Flow Cytometer system (Thermo Fisher Scientific).

KW - Attune Nxt

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A standarized protocol for evaluation of large extracellular vesicles using the attune™ NXT system

Abstract

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