Abstract
Restriction digestion is routinely performed in a buffer compatible with the restriction enzyme used. To load the samples on agarose gels for electrophoresis it is then necessary to add a loading buffer. A 10X loading buffer is often used, and consists of a dye to track the electrophoresis and a dense solution so that the digestion mixture sinks into the well. We describe a new buffer, which acts as a universal digestion buffer as well as a loading, buffer. This avoids double handling of samples, which wastes both time and consumables. Importantly, the efficiency of digestion was found not to be significantly decreased in this new buffer.
Original language | English |
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Pages (from-to) | 51-55 |
Journal | Molecular Biotechnology |
Volume | 21 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2002 |