A simplified and inexpensive method for the preparation of fresh or fixed bone samples for forensic histological examination

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Abstract

The histological analysis of bone for forensic application requires thin sections to be prepared from extracted bone of suitable quality for visualization through conventional light microscopy and (if required) the measurement of microstructural features, such as osteons. While a number of protocols for the preparation of thin sections exist, they are often time consuming and complex, or require expensive and non-portable equipment. A revised protocol is presented for obtaining high quality thin sections for light microscopy and histological analyses, tested in fresh, fixed, and dry archaeological cortical bone. It does not require extensive experience, or expensive materials or equipment, and can be further adapted for use with manual tools if necessary.
Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalAustralian Journal of Forensic Sciences
Volume51
Issue numbersup1
DOIs
Publication statusE-pub ahead of print - 12 Feb 2019

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Microscopy
Haversian System
Light
Bone and Bones
Equipment and Supplies
Cortical Bone

Cite this

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title = "A simplified and inexpensive method for the preparation of fresh or fixed bone samples for forensic histological examination",
abstract = "The histological analysis of bone for forensic application requires thin sections to be prepared from extracted bone of suitable quality for visualization through conventional light microscopy and (if required) the measurement of microstructural features, such as osteons. While a number of protocols for the preparation of thin sections exist, they are often time consuming and complex, or require expensive and non-portable equipment. A revised protocol is presented for obtaining high quality thin sections for light microscopy and histological analyses, tested in fresh, fixed, and dry archaeological cortical bone. It does not require extensive experience, or expensive materials or equipment, and can be further adapted for use with manual tools if necessary.",
author = "Ariane Maggio and Ambika Flavel and Daniel Franklin",
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