A simple bacterial bioassay for the measurement of L-glutamine

David Keast; W. Harper; D.G. Rowbottom; J.E. Greig; S. Kargotich; S. Mcbride

doi:10.1177/000456329803500115

A simple bacterial bioassay for the measurement of L-glutamine

David Keast, W. Harper, D.G. Rowbottom, J.E. Greig, S. Kargotich, S. Mcbride

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The amino acid L-glutamine has been shown to be important to numerous cells in the body. However, the routine measurement of glutamine in biological solutions is complicated by its relative instability, particularly at extremes of pH. We report a simple bacterial bioassay method for the estimation of glutamine, carried out at neutral pH, using an Escherichia coli that is specifically dependent on the presence of glutamine for replication. The assay is simple to perform and we have shown it to be highly reproducible. Furthermore, we observed significant correlation between the estimations of glutamine by the bioassay system and current methodologies. The levels of glutamine recorded were higher than by other methods. We also found that deproteinization and neutralization of samples allowed them to be stored at -70 degrees C for up to 24 weeks without deterioration.

Original language	English
Pages (from-to)	110-114
Journal	Annals of Clinical Biochemistry
Volume	35
DOIs	https://doi.org/10.1177/000456329803500115
Publication status	Published - 1998

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title = "A simple bacterial bioassay for the measurement of L-glutamine",

abstract = "The amino acid L-glutamine has been shown to be important to numerous cells in the body. However, the routine measurement of glutamine in biological solutions is complicated by its relative instability, particularly at extremes of pH. We report a simple bacterial bioassay method for the estimation of glutamine, carried out at neutral pH, using an Escherichia coli that is specifically dependent on the presence of glutamine for replication. The assay is simple to perform and we have shown it to be highly reproducible. Furthermore, we observed significant correlation between the estimations of glutamine by the bioassay system and current methodologies. The levels of glutamine recorded were higher than by other methods. We also found that deproteinization and neutralization of samples allowed them to be stored at -70 degrees C for up to 24 weeks without deterioration.",

author = "David Keast and W. Harper and D.G. Rowbottom and J.E. Greig and S. Kargotich and S. Mcbride",

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T1 - A simple bacterial bioassay for the measurement of L-glutamine

AU - Keast, David

AU - Harper, W.

AU - Rowbottom, D.G.

AU - Greig, J.E.

AU - Kargotich, S.

AU - Mcbride, S.

PY - 1998

Y1 - 1998

N2 - The amino acid L-glutamine has been shown to be important to numerous cells in the body. However, the routine measurement of glutamine in biological solutions is complicated by its relative instability, particularly at extremes of pH. We report a simple bacterial bioassay method for the estimation of glutamine, carried out at neutral pH, using an Escherichia coli that is specifically dependent on the presence of glutamine for replication. The assay is simple to perform and we have shown it to be highly reproducible. Furthermore, we observed significant correlation between the estimations of glutamine by the bioassay system and current methodologies. The levels of glutamine recorded were higher than by other methods. We also found that deproteinization and neutralization of samples allowed them to be stored at -70 degrees C for up to 24 weeks without deterioration.

AB - The amino acid L-glutamine has been shown to be important to numerous cells in the body. However, the routine measurement of glutamine in biological solutions is complicated by its relative instability, particularly at extremes of pH. We report a simple bacterial bioassay method for the estimation of glutamine, carried out at neutral pH, using an Escherichia coli that is specifically dependent on the presence of glutamine for replication. The assay is simple to perform and we have shown it to be highly reproducible. Furthermore, we observed significant correlation between the estimations of glutamine by the bioassay system and current methodologies. The levels of glutamine recorded were higher than by other methods. We also found that deproteinization and neutralization of samples allowed them to be stored at -70 degrees C for up to 24 weeks without deterioration.

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DO - 10.1177/000456329803500115

M3 - Article

VL - 35

SP - 110

EP - 114

JO - Annals of Clinical Biochemistry

JF - Annals of Clinical Biochemistry

ER -

A simple bacterial bioassay for the measurement of L-glutamine

Abstract

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