A Selective Inhibitor Gal-PUGNAc of Human Lysosomal ß-Hexosaminidases Modulates Levels of the Ganglioside GM2 in Neuroblastoma Cells

Keith Stubbs; M.S. Macauley; D.J. Vocadlo

doi:10.1002/anie.200804583

A Selective Inhibitor Gal-PUGNAc of Human Lysosomal ß-Hexosaminidases Modulates Levels of the Ganglioside GM2 in Neuroblastoma Cells

Keith Stubbs, M.S. Macauley, D.J. Vocadlo

School of Molecular Sciences

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

Gal-PUGNAc (see picture), a highly selective inhibitor for β-hexosaminidases HEXA and HEXB is cell-permeable and modulates the activity of HEXA and HEXB in tissue culture, increasing ganglioside GM2 levels. Gal-PUGNAc should allow the role of these enzymes to be studied at the cellular level without generating a complex chemical phenotype from concomitant inhibition of O-GlcNAcase.

Original language	English
Pages (from-to)	1300-1303
Journal	Angewandte Chemie (International Edition)
Volume	48
Issue number	7
DOIs	https://doi.org/10.1002/anie.200804583
Publication status	Published - 2009

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abstract = "Gal-PUGNAc (see picture), a highly selective inhibitor for β-hexosaminidases HEXA and HEXB is cell-permeable and modulates the activity of HEXA and HEXB in tissue culture, increasing ganglioside GM2 levels. Gal-PUGNAc should allow the role of these enzymes to be studied at the cellular level without generating a complex chemical phenotype from concomitant inhibition of O-GlcNAcase.",

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AB - Gal-PUGNAc (see picture), a highly selective inhibitor for β-hexosaminidases HEXA and HEXB is cell-permeable and modulates the activity of HEXA and HEXB in tissue culture, increasing ganglioside GM2 levels. Gal-PUGNAc should allow the role of these enzymes to be studied at the cellular level without generating a complex chemical phenotype from concomitant inhibition of O-GlcNAcase.

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ER -

A Selective Inhibitor Gal-PUGNAc of Human Lysosomal ß-Hexosaminidases Modulates Levels of the Ganglioside GM2 in Neuroblastoma Cells

Abstract

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