Gal-PUGNAc (see picture), a highly selective inhibitor for β-hexosaminidases HEXA and HEXB is cell-permeable and modulates the activity of HEXA and HEXB in tissue culture, increasing ganglioside GM2 levels. Gal-PUGNAc should allow the role of these enzymes to be studied at the cellular level without generating a complex chemical phenotype from concomitant inhibition of O-GlcNAcase.
Stubbs, K., Macauley, M. S., & Vocadlo, D. J. (2009). A Selective Inhibitor Gal-PUGNAc of Human Lysosomal ß-Hexosaminidases Modulates Levels of the Ganglioside GM2 in Neuroblastoma Cells. Angewandte Chemie (International Edition), 48(7), 1300-1303. https://doi.org/10.1002/anie.200804583