A requirement for DNA synthesis in foetal hepatocyte differentiation: Effect of cytosine arabinoside on the appearance of the liver isoenzyme of pyruvate kinase

Rodney Scott, George C.T. Yeoh

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    In hepatocyte cultures derived from 15-day-old foetal rats, the appearance of the liver (L) form of pyruvate kinase is blocked when cytosine arabinoside is added on the 2nd day of culture. When added on the 3rd day of culture, the inhibitor of DNA synthesis does not prevent the appearance of the enzyme. If cytosine arabinoside is added on the 2nd day of culture and removed on the 4th day, the enzyme is detected by the 6th day of culture. The specificity of the action of cytosine arabinoside for the L form of pyruvate kinase is in contrast with the lack of effect observed on total protein synthesis and the activity of the embryonic (M2) form of the enzyme.

    Original languageEnglish
    Pages (from-to)49-52
    Number of pages4
    JournalDifferentiation
    Volume28
    Issue number1
    DOIs
    Publication statusPublished - 1 Jan 1984

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