Field hockey is a high-intensity intermittent team sport that has recently undergone a series of rule changes that have resulted in a greater demand for repeated high-intensity movements. Coaches and practitioners now require a reliable assessment of repeated accelerations, decelerations and changes of direction to assess these important match qualities. This investigation assessed the test-retest reliability of a novel 6x40m repeated shuttle sprint test (20m + 20m with a 180turn) and its association with 40m straight line sprint and YoYo Intermittent Recovery Test performance in 28 International field hockey players (n = 14 females and n = 14 males). The sum of 6 sprint times (SUM) demonstrated ‘excellent’ (ICC = 0.94 and CV = 0.59%) and ‘good’ (ICC = 0.84 and CV = 0.75%) reliability in females and males, respectively. Best sprint time during the repeated shuttle sprint test also demonstrated suitable reliability to evaluate field hockey physical performance (ICC = 0.92 & 0.76, CV = 0.76% & 1.00% in females and males, respectively). SUM was significantly associated with 40 m straight line sprint performance in females (r = 0.90; p<0.001) and males (r = 0.92; p<0.001), but only a weak association was found with YoYo Intermittent Recovery Test performance for either group (r = 0.20; p = 0.495 & r = -0.19; p = 0.525 in females and males, respectively). In summary, field hockey testing batteries that include a repeated shuttle sprint test should consider including a test of intermittent endurance. Further, changes in SUM greater than 1.0% can be confidently interpreted by coaches and practitioners as a real change for both female and male elite field hockey players.