Objective:To develop a polymerase chain reaction (PCR) strategy to screen the dystrophin promoter(s) in dogs with cardiac and skeletal myopathies.Animals-9 Doberman Pinschers; 1 Dalmation, and ? Saint Bernard with dilated cardiomyopathy (DCM)1 Irish Terrier with muscular dystrophy; and 2 dystrophin-deficient German Shorthaired Pointers (GSHP).Procedure-For each of the 3 unique exons associated with the muscle (M), Purkinje (P), and cortical (C) promoters of the dystrophin gene, each first exon, and the M promoter plus its first exon, were amplified, cloned, and sequenced. The M dystrophin transcript was amplified by reverse transcriptase PCR from skeletal and cardiac muscle RNA of 1 Doberman Pinscher and from skeletal muscle RNA of 1 GSHP.Results-The M, P,and C first exons were amplified from all dogs except the 2 GSHP, which had a deletion encompassing-the entire M, P, and C dystrophin promoter region. The M transcript could not be amplified from muscles of-the GSHP, but was amplified from skeletal and cardiac muscle of the Doberman Pinscher. Sequencing of the product representing the M promoter and its first exon revealed no differences between clinically normal dogs and the Doberman' Pinscher with DCM.Conclusions and Clinical Relevance-We have ruled out a major rearrangement of the dystrophin promoter region as the universal cause of DCM in Doberman Pinschers or of Irish Terrier myopathy. Use of the strategy identified a large deletion of this region in muscle from the GSHP.
|Journal||American Journal of Veterinary Research|
|Publication status||Published - 1999|
Schatzberg, S. J., Olby, N. J., Steingold, S., Keene, B., Atkins, C., Meurs, K., Solomon, G., Goedegebuure, S. A., Wilton, S., & Sharp, N. J. (1999). A polymerase chain reaction screening strategy for the promoter of the canine dystrophin gene. American Journal of Veterinary Research, 60(9), 1040-1046.