A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery

Yaming Gu; Yizhen Guo; Changyong Wang; Jiake Xu; Jianping Wu; Thomas Brett Kirk; Dong Ma; Wei Xue

doi:10.1016/j.msec.2016.09.035

A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery

Yaming Gu, Yizhen Guo, Changyong Wang, Jiake Xu, Jianping Wu, Thomas Brett Kirk, Dong Ma, Wei Xue

Research output: Contribution to journal › Article

42 Citations (Scopus)

Abstract

It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective treatment to breast cancer. GO-PAMAM has a high loading capacity to DOX and pH-controlled DOX release. Besides, it has efficient gene transfer ability, the transfection efficiency is significantly better than PEI-25k in the presence of serum, and it can significantly inhibit the expression of MMP-9 protein in MCF-7 cells. The effect of DOX and MMP-9 shRNA plasmid co-delivery was more significant than that of the single drug. Moreover, GO-PAMAM exhibited lower cytotoxicity compared to PEI-25k in CCK-8 assays, and also showed a good biocompatibility in vivo. Therefore, GO-PAMAM will have broad prospects for drug and gene co-delivery.

Original language	English
Pages (from-to)	572-585
Number of pages	14
Journal	Materials Science and Engineering C
Volume	70
DOIs	https://doi.org/10.1016/j.msec.2016.09.035
Publication status	Published - 1 Jan 2017

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Gu, Y., Guo, Y., Wang, C., Xu, J., Wu, J., Kirk, T. B., Ma, D., & Xue, W. (2017). A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery. Materials Science and Engineering C, 70, 572-585. https://doi.org/10.1016/j.msec.2016.09.035

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title = "A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery",

abstract = "It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective treatment to breast cancer. GO-PAMAM has a high loading capacity to DOX and pH-controlled DOX release. Besides, it has efficient gene transfer ability, the transfection efficiency is significantly better than PEI-25k in the presence of serum, and it can significantly inhibit the expression of MMP-9 protein in MCF-7 cells. The effect of DOX and MMP-9 shRNA plasmid co-delivery was more significant than that of the single drug. Moreover, GO-PAMAM exhibited lower cytotoxicity compared to PEI-25k in CCK-8 assays, and also showed a good biocompatibility in vivo. Therefore, GO-PAMAM will have broad prospects for drug and gene co-delivery.",

keywords = "Co-delivery, Dendrimer, DOX, Graphene oxide, MMP-9 shRNA plasmid",

author = "Yaming Gu and Yizhen Guo and Changyong Wang and Jiake Xu and Jianping Wu and Kirk, {Thomas Brett} and Dong Ma and Wei Xue",

year = "2017",

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doi = "10.1016/j.msec.2016.09.035",

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AU - Gu, Yaming

AU - Guo, Yizhen

AU - Wang, Changyong

AU - Xu, Jiake

AU - Wu, Jianping

AU - Kirk, Thomas Brett

AU - Ma, Dong

AU - Xue, Wei

PY - 2017/1/1

Y1 - 2017/1/1

N2 - It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective treatment to breast cancer. GO-PAMAM has a high loading capacity to DOX and pH-controlled DOX release. Besides, it has efficient gene transfer ability, the transfection efficiency is significantly better than PEI-25k in the presence of serum, and it can significantly inhibit the expression of MMP-9 protein in MCF-7 cells. The effect of DOX and MMP-9 shRNA plasmid co-delivery was more significant than that of the single drug. Moreover, GO-PAMAM exhibited lower cytotoxicity compared to PEI-25k in CCK-8 assays, and also showed a good biocompatibility in vivo. Therefore, GO-PAMAM will have broad prospects for drug and gene co-delivery.

AB - It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective treatment to breast cancer. GO-PAMAM has a high loading capacity to DOX and pH-controlled DOX release. Besides, it has efficient gene transfer ability, the transfection efficiency is significantly better than PEI-25k in the presence of serum, and it can significantly inhibit the expression of MMP-9 protein in MCF-7 cells. The effect of DOX and MMP-9 shRNA plasmid co-delivery was more significant than that of the single drug. Moreover, GO-PAMAM exhibited lower cytotoxicity compared to PEI-25k in CCK-8 assays, and also showed a good biocompatibility in vivo. Therefore, GO-PAMAM will have broad prospects for drug and gene co-delivery.

KW - Co-delivery

KW - Dendrimer

KW - DOX

KW - Graphene oxide

KW - MMP-9 shRNA plasmid

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