A novel tomato Fusarium wilt tolerance gene

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The reduced mycorrhizal colonization (rmc) tomato mutant is unable to form mycorrhiza and is more susceptible to Fusarium wilt compared with its wild-type isogenic line 76R. The rmc mutant has a chromosomal deletion affecting five genes, one of which is similar to CYCLOPS. Loss of this gene is responsible for non-mycorrhizality in rmc but not enhanced Fusarium wilt susceptibility. Here, we describe assessment of a second gene in the rmc deletion, designated Solyc08g075770 that is expressed in roots. Sequence analyses show that Solyc08g075770 encodes a small transmembrane protein with putative phosphorylation and glycosylation sites. It is predicted to be localized in the plasma membrane and may function in transmembrane ion transport and/or as a cell surface receptor. Complementation and knock-out strategies were used to test its function. Some putative CRISPR/Cas-9 knock-out transgenic events exhibited Fusarium wilt susceptibility like rmc and some putative complementation lines were 76R-like, suggesting that the tomato Solyc08g075770 functions in Fusarium wilt tolerance. This is the first study to demonstrate that Solyc08g075770 is the contributor to the Tfw locus, conferring tolerance to Fusarium wilt in 76R which was lost in rmc.

Original languageEnglish
Article number1226
JournalFrontiers in Microbiology
Volume9
Issue numberJUN
DOIs
Publication statusPublished - 8 Jun 2018

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Fusarium
Lycopersicon esculentum
Genes
Clustered Regularly Interspaced Short Palindromic Repeats
Mycorrhizae
Ion Transport
Cell Surface Receptors
Glycosylation
Sequence Analysis
Phosphorylation
Cell Membrane
Proteins

Cite this

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title = "A novel tomato Fusarium wilt tolerance gene",
abstract = "The reduced mycorrhizal colonization (rmc) tomato mutant is unable to form mycorrhiza and is more susceptible to Fusarium wilt compared with its wild-type isogenic line 76R. The rmc mutant has a chromosomal deletion affecting five genes, one of which is similar to CYCLOPS. Loss of this gene is responsible for non-mycorrhizality in rmc but not enhanced Fusarium wilt susceptibility. Here, we describe assessment of a second gene in the rmc deletion, designated Solyc08g075770 that is expressed in roots. Sequence analyses show that Solyc08g075770 encodes a small transmembrane protein with putative phosphorylation and glycosylation sites. It is predicted to be localized in the plasma membrane and may function in transmembrane ion transport and/or as a cell surface receptor. Complementation and knock-out strategies were used to test its function. Some putative CRISPR/Cas-9 knock-out transgenic events exhibited Fusarium wilt susceptibility like rmc and some putative complementation lines were 76R-like, suggesting that the tomato Solyc08g075770 functions in Fusarium wilt tolerance. This is the first study to demonstrate that Solyc08g075770 is the contributor to the Tfw locus, conferring tolerance to Fusarium wilt in 76R which was lost in rmc.",
keywords = "Fusarium oxysporum f. sp. lycopersici, Fusarium wilt, Mycorrhizal symbiosis, Solanum lycopersicum, Tolerance, Tomato transformation",
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A novel tomato Fusarium wilt tolerance gene. / Prihatna, Cahya; Barbetti, Martin J.; Barker, Susan J.

In: Frontiers in Microbiology, Vol. 9, No. JUN, 1226, 08.06.2018.

Research output: Contribution to journalArticle

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