A novel mutation in the steroidogenic acute regulatory protein gene promoter leading to reduced promoter activity

A.J. Casal, V.J.P. Sinclair, A.M. Capponi, J. Nicod, U. Huynh-Do, Paolo Ferrari

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    We have identified a novel cytosine/thymidine polymorphism of the human steroidogenic acute regulatory (StAR) gene promoter located 3 bp downstream of the steroidogenic factor-1 (SF-1)-binding site and 9 bp upstream of the TATA box (ATTTAAG). Carriers of this mutation have a high prevalence of primary aldosteronism. In transfection experiments, basal StAR promoter activity was unaltered by the mutation in murine Y-1 cells and human H295R cells. In Y-1 cells, forskolin (25 mu M, 6 h) significantly increased wild-type promoter activity to 230 +/- 33% (P < 0.05, n=4). In contrast, forskolin increased mutated promoter activity only to 150 +/- 27%, with a significant 35% reduction compared to wild type (P < 0(.)05, n=3). In H295R cells, angiotensin II (AngII; 10 nM) increased wild-type StAR promoter activity to 265 +/- 22% (P < 0(.)01, n=3), while mutated StAR promoter activity in response to AngII only reached 180 +/- 29% of controls (P < 0(.)01, n=3). Gel mobility shift assays show the formation of two additional complexes with the mutated promoter: one with the transcription repressor DAX-1 and another with a yet unidentified factor, which strongly binds the SF-1 response element. Thus, this novel mutation in the human StAR promoter is critically involved in the regulation of StAR gene expression and is associated with reduced promoter activity, a finding relevant for adrenal steroid response to physiological stimulators.
    Original languageEnglish
    Pages (from-to)71-80
    JournalJournal of Molecular Endocrinology
    Volume37
    DOIs
    Publication statusPublished - 2006

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