A Novel Approach to Authentication and Quality Control of Western Australian Honeys using High Performance Thin Layer Chromatography

Md Khairul Islam

doi:10.26182/3k2d-2h55

A Novel Approach to Authentication and Quality Control of Western Australian Honeys using High Performance Thin Layer Chromatography

Md Khairul Islam

School of Allied Health

Research output: Thesis › Doctoral Thesis

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Abstract

Authentication and quality control of honeys are essential but challenging processes, especially for honeys collected from botanically rich areas, such as Western Australia's pristine natural vegetation. Within this specific regional context, this study explores the use of High-Performance Thin Layer Chromatography (HPTLC) as a novel analytical tool for (1) the authentication of honey's predominant floral source via HPTLC fingerprinting of its organic extract; (2) the detection and quantification of honey constituents that contribute to its antioxidant activity via a novel, validated HPTLC-DPPH analysis; and (3) the qualitative and quantitative analysis of its major sugars using a new validated HPTLC assay.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Locher, Connie, Supervisor Hammer, Kate, Supervisor Lim, Lee Yong, Supervisor
Award date	20 Dec 2022
DOIs	https://doi.org/10.26182/3k2d-2h55
Publication status	Unpublished - 2022

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10.26182/3k2d-2h55

THESIS - DOCTOR OF PHILOSOPHY - ISLAM, Md Khairul - 2022
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8 Article

A Comprehensive HPTLC-Based Analysis of the Impacts of Temperature on the Chemical Properties and Antioxidant Activity of Honey
Islam, M. K., Sostaric, T., Lim, L. Y., Hammer, K. & Locher, C., 2 Dec 2022, In: Molecules (Basel, Switzerland). 27, 23, 24 p., 8491.
Research output: Contribution to journal › Article › peer-review

Open Access
3 Citations (Scopus)
An investigation of the suitability of melissopalynology to authenticate Jarrah honey
Islam, M. K., Lawag, I., Green, K., Sostaric, T., Hammer, K., Lim, L. Y. & Locher, C., Jan 2022, In: Current Research in Food Science. 5, p. 506-514 9 p.
Research output: Contribution to journal › Article › peer-review

Open Access
12 Citations (Scopus)
Antioxidant HPTLC-DPPH Fingerprinting of Honeys and Tracking of Antioxidant Constituents Upon Thermal Exposure
Islam, M. K., Sostaric, T., Lim, L. Y., Hammer, K. & Locher, C., 7 Feb 2021, In: Foods. 10, 2, 357.
Research output: Contribution to journal › Article › peer-review

Open Access
15 Citations (Scopus)

Cite this

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title = "A Novel Approach to Authentication and Quality Control of Western Australian Honeys using High Performance Thin Layer Chromatography",

abstract = "Authentication and quality control of honeys are essential but challenging processes, especially for honeys collected from botanically rich areas, such as Western Australia's pristine natural vegetation. Within this specific regional context, this study explores the use of High-Performance Thin Layer Chromatography (HPTLC) as a novel analytical tool for (1) the authentication of honey's predominant floral source via HPTLC fingerprinting of its organic extract; (2) the detection and quantification of honey constituents that contribute to its antioxidant activity via a novel, validated HPTLC-DPPH analysis; and (3) the qualitative and quantitative analysis of its major sugars using a new validated HPTLC assay.",

keywords = "honey, Western Australia, HPTLC, quality control, authentication, fingerprints",

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doi = "10.26182/3k2d-2h55",

language = "English",

school = "The University of Western Australia",

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AB - Authentication and quality control of honeys are essential but challenging processes, especially for honeys collected from botanically rich areas, such as Western Australia's pristine natural vegetation. Within this specific regional context, this study explores the use of High-Performance Thin Layer Chromatography (HPTLC) as a novel analytical tool for (1) the authentication of honey's predominant floral source via HPTLC fingerprinting of its organic extract; (2) the detection and quantification of honey constituents that contribute to its antioxidant activity via a novel, validated HPTLC-DPPH analysis; and (3) the qualitative and quantitative analysis of its major sugars using a new validated HPTLC assay.

KW - honey

KW - Western Australia

KW - HPTLC

KW - quality control

KW - authentication

KW - fingerprints

U2 - 10.26182/3k2d-2h55

DO - 10.26182/3k2d-2h55

M3 - Doctoral Thesis

ER -

A Novel Approach to Authentication and Quality Control of Western Australian Honeys using High Performance Thin Layer Chromatography

Abstract

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Research output

A Comprehensive HPTLC-Based Analysis of the Impacts of Temperature on the Chemical Properties and Antioxidant Activity of Honey

An investigation of the suitability of melissopalynology to authenticate Jarrah honey

Antioxidant HPTLC-DPPH Fingerprinting of Honeys and Tracking of Antioxidant Constituents Upon Thermal Exposure

Cite this