A novel antimicrobial hydrogel for the management of periodontal diseases: Antimicrobial Hydrogel to treat Periodontitis

Pauline Wong; Shane  Soo; Edmund Wong; Praveen Praveen; Peta Clode; Murray Baker; Victor Haruo Matsubara

doi:10.1016/j.identj.2023.01.002

A novel antimicrobial hydrogel for the management of periodontal diseases: Antimicrobial Hydrogel to treat Periodontitis

Pauline Wong, Shane Soo, Edmund Wong, Praveen Praveen, Peta Clode, Murray Baker, Victor Haruo Matsubara

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Objectives: This study aimed to synthesise a drug-delivery system based on a porous polymer hydrogel, with antimicrobial properties against Porphyromonas gingivalis and potential to be used in tissue regeneration.

Material and methods: 2-Hydroxyethyl methacrylate monomers were polymerised using thermal and photoactivation in the presence of silver nitrate (AgNO3) and/or chlorhexidine digluconate. Poly-2-hydroxyethyl methacrylate (pHEMA) hydrogels containing silver nanoparticles (AgNPs) and/or 0.12% chlorhexidine (CHX) were produced and characterised using cryo-SEM and confocal microscopy. Hydrogel degradation and leaching of AgNP were tested for 1.5 months. The antimicrobial properties were tested against P. gingivalis using broth culture system and disk diffusion tests.

Results: Our methodology manufactured porous polymeric hydrogels doped with AgNPs and CHX. Hydrogels showed a successful delivery of CHX and sustainable release of AgNPs in a steady hydrogel degradation rate determined based on the weight loss of samples. Hydrogels with AgNPs or CHX had a significant antimicrobial effect against P. gingivalis, with CHX-hydrogels exhibiting a stronger effect than AgNP-hydrogels in the short-term assessment. AgNP-CHX hydrogels showed a compounded antimicrobial effect, whereas control hydrogels containing neither AgNPs nor CHX had no influence on bacterial growth(P < .05).

Conclusions: The dual-cured pHEMA hydrogel loaded with antimicrobial agents proved to bean efficient drug-delivery system against periodontopathogens, with the potential to be used as a scaffold for tissue regeneration.

Original language	English
Pages (from-to)	354-361
Number of pages	8
Journal	International Dental Journal
Volume	73
Issue number	3
DOIs	https://doi.org/10.1016/j.identj.2023.01.002
Publication status	Published - Jun 2023

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@article{cae32743f6c446a3af749f9dd50c32d0,

title = "A novel antimicrobial hydrogel for the management of periodontal diseases: Antimicrobial Hydrogel to treat Periodontitis",

abstract = "Objectives: This study aimed to synthesise a drug-delivery system based on a porous polymer hydrogel, with antimicrobial properties against Porphyromonas gingivalis and potential to be used in tissue regeneration. Material and methods: 2-Hydroxyethyl methacrylate monomers were polymerised using thermal and photoactivation in the presence of silver nitrate (AgNO3) and/or chlorhexidine digluconate. Poly-2-hydroxyethyl methacrylate (pHEMA) hydrogels containing silver nanoparticles (AgNPs) and/or 0.12% chlorhexidine (CHX) were produced and characterised using cryo-SEM and confocal microscopy. Hydrogel degradation and leaching of AgNP were tested for 1.5 months. The antimicrobial properties were tested against P. gingivalis using broth culture system and disk diffusion tests. Results: Our methodology manufactured porous polymeric hydrogels doped with AgNPs and CHX. Hydrogels showed a successful delivery of CHX and sustainable release of AgNPs in a steady hydrogel degradation rate determined based on the weight loss of samples. Hydrogels with AgNPs or CHX had a significant antimicrobial effect against P. gingivalis, with CHX-hydrogels exhibiting a stronger effect than AgNP-hydrogels in the short-term assessment. AgNP-CHX hydrogels showed a compounded antimicrobial effect, whereas control hydrogels containing neither AgNPs nor CHX had no influence on bacterial growth(P < .05). Conclusions: The dual-cured pHEMA hydrogel loaded with antimicrobial agents proved to bean efficient drug-delivery system against periodontopathogens, with the potential to be used as a scaffold for tissue regeneration.",

keywords = "Antimicrobial agent, Chlorhexidine, Hydrogel, Silver nanoparticles, Tissue scaffolds",

author = "Pauline Wong and Shane Soo and Edmund Wong and Praveen Praveen and Peta Clode and Murray Baker and {Haruo Matsubara}, Victor",

note = "Funding Information: The authors acknowledge the facilities and the scientific and technical assistance of Microscopy Australia at the Centre for Microscopy, Characterisation & Analysis, The University of Western Australia, a facility funded by the University, State, and Commonwealth Governments. The authors also thank Dr Kate Shearston for the technical support in the UWA Dental School research laboratory as well as the Colgate Research Grant and the Australian Dental Research Foundation for their contributions and invaluable support for this study. Pauline Wong, Shane Soo, and Edmund Wong contributed equally to this study. Funding Information: The authors acknowledge the facilities and the scientific and technical assistance of Microscopy Australia at the Centre for Microscopy, Characterisation & Analysis, The University of Western Australia, a facility funded by the University, State, and Commonwealth Governments. The authors also thank Dr Kate Shearston for the technical support in the UWA Dental School research laboratory as well as the Colgate Research Grant and the Australian Dental Research Foundation for their contributions and invaluable support for this study. Publisher Copyright: {\textcopyright} 2023 The Authors",

year = "2023",

month = jun,

doi = "10.1016/j.identj.2023.01.002",

language = "English",

volume = "73",

pages = "354--361",

journal = "International Dental Journal",

issn = "0020-6539",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - A novel antimicrobial hydrogel for the management of periodontal diseases

T2 - Antimicrobial Hydrogel to treat Periodontitis

AU - Wong, Pauline

AU - Soo, Shane

AU - Wong, Edmund

AU - Praveen, Praveen

AU - Clode, Peta

AU - Baker, Murray

AU - Haruo Matsubara, Victor

N1 - Funding Information: The authors acknowledge the facilities and the scientific and technical assistance of Microscopy Australia at the Centre for Microscopy, Characterisation & Analysis, The University of Western Australia, a facility funded by the University, State, and Commonwealth Governments. The authors also thank Dr Kate Shearston for the technical support in the UWA Dental School research laboratory as well as the Colgate Research Grant and the Australian Dental Research Foundation for their contributions and invaluable support for this study. Pauline Wong, Shane Soo, and Edmund Wong contributed equally to this study. Funding Information: The authors acknowledge the facilities and the scientific and technical assistance of Microscopy Australia at the Centre for Microscopy, Characterisation & Analysis, The University of Western Australia, a facility funded by the University, State, and Commonwealth Governments. The authors also thank Dr Kate Shearston for the technical support in the UWA Dental School research laboratory as well as the Colgate Research Grant and the Australian Dental Research Foundation for their contributions and invaluable support for this study. Publisher Copyright: © 2023 The Authors

PY - 2023/6

Y1 - 2023/6

N2 - Objectives: This study aimed to synthesise a drug-delivery system based on a porous polymer hydrogel, with antimicrobial properties against Porphyromonas gingivalis and potential to be used in tissue regeneration. Material and methods: 2-Hydroxyethyl methacrylate monomers were polymerised using thermal and photoactivation in the presence of silver nitrate (AgNO3) and/or chlorhexidine digluconate. Poly-2-hydroxyethyl methacrylate (pHEMA) hydrogels containing silver nanoparticles (AgNPs) and/or 0.12% chlorhexidine (CHX) were produced and characterised using cryo-SEM and confocal microscopy. Hydrogel degradation and leaching of AgNP were tested for 1.5 months. The antimicrobial properties were tested against P. gingivalis using broth culture system and disk diffusion tests. Results: Our methodology manufactured porous polymeric hydrogels doped with AgNPs and CHX. Hydrogels showed a successful delivery of CHX and sustainable release of AgNPs in a steady hydrogel degradation rate determined based on the weight loss of samples. Hydrogels with AgNPs or CHX had a significant antimicrobial effect against P. gingivalis, with CHX-hydrogels exhibiting a stronger effect than AgNP-hydrogels in the short-term assessment. AgNP-CHX hydrogels showed a compounded antimicrobial effect, whereas control hydrogels containing neither AgNPs nor CHX had no influence on bacterial growth(P < .05). Conclusions: The dual-cured pHEMA hydrogel loaded with antimicrobial agents proved to bean efficient drug-delivery system against periodontopathogens, with the potential to be used as a scaffold for tissue regeneration.

AB - Objectives: This study aimed to synthesise a drug-delivery system based on a porous polymer hydrogel, with antimicrobial properties against Porphyromonas gingivalis and potential to be used in tissue regeneration. Material and methods: 2-Hydroxyethyl methacrylate monomers were polymerised using thermal and photoactivation in the presence of silver nitrate (AgNO3) and/or chlorhexidine digluconate. Poly-2-hydroxyethyl methacrylate (pHEMA) hydrogels containing silver nanoparticles (AgNPs) and/or 0.12% chlorhexidine (CHX) were produced and characterised using cryo-SEM and confocal microscopy. Hydrogel degradation and leaching of AgNP were tested for 1.5 months. The antimicrobial properties were tested against P. gingivalis using broth culture system and disk diffusion tests. Results: Our methodology manufactured porous polymeric hydrogels doped with AgNPs and CHX. Hydrogels showed a successful delivery of CHX and sustainable release of AgNPs in a steady hydrogel degradation rate determined based on the weight loss of samples. Hydrogels with AgNPs or CHX had a significant antimicrobial effect against P. gingivalis, with CHX-hydrogels exhibiting a stronger effect than AgNP-hydrogels in the short-term assessment. AgNP-CHX hydrogels showed a compounded antimicrobial effect, whereas control hydrogels containing neither AgNPs nor CHX had no influence on bacterial growth(P < .05). Conclusions: The dual-cured pHEMA hydrogel loaded with antimicrobial agents proved to bean efficient drug-delivery system against periodontopathogens, with the potential to be used as a scaffold for tissue regeneration.

KW - Antimicrobial agent

KW - Chlorhexidine

KW - Hydrogel

KW - Silver nanoparticles

KW - Tissue scaffolds

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U2 - 10.1016/j.identj.2023.01.002

DO - 10.1016/j.identj.2023.01.002

M3 - Article

C2 - 36754776

SN - 0020-6539

VL - 73

SP - 354

EP - 361

JO - International Dental Journal

JF - International Dental Journal

IS - 3

ER -

A novel antimicrobial hydrogel for the management of periodontal diseases: Antimicrobial Hydrogel to treat Periodontitis

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