TY - JOUR
T1 - A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61 beta subunit
AU - Ingley, Evan
AU - Williams, J.H.
AU - Walker, C.E.
AU - Tsai, S.
AU - Colley, S.
AU - Sayer, M.S.
AU - Tilbrook, Peta
AU - Sarna, M.
AU - Beaumont, Jennifer
AU - Klinken, Peter
PY - 1999
Y1 - 1999
N2 - We report here the isolation of a new member of the ADP-ribosylation factor (ARF)-like family (ARL-6) present in the J2E erythroleukemic cell line, but not its myeloid variants, Consistent with this lineage-restricted expression, ARL-6 mRNA increased with erythropoietin-induced maturation of J2E cells, and decreased with interleukin 6-induced differentiation of M1 monoblastoid cells. In tissues, ARL-6 mRNA was most abundant in brain and kidney. While ARL-6 protein was predominantly cytosolic, its membrane association increased following exposure to GTP-gamma S, like many members of the ARF/ARL family. Using the yeast two-hybrid system, six molecules which interact with ARL-6 were identified including SEC61 beta, a subunit of the heterotrimeric protein conducting channel SEC61 beta, Co-immunoprecipitation of ARL-6 confirmed a stable association between ARL-6 and SEC61 beta in COS cells. These results demonstrate that ARL-6, a novel member of the ADP-ribosylation factor-like family, interacts with the SEC61 beta subunit, (C) 1999 Federation of European Biochemical Societies.
AB - We report here the isolation of a new member of the ADP-ribosylation factor (ARF)-like family (ARL-6) present in the J2E erythroleukemic cell line, but not its myeloid variants, Consistent with this lineage-restricted expression, ARL-6 mRNA increased with erythropoietin-induced maturation of J2E cells, and decreased with interleukin 6-induced differentiation of M1 monoblastoid cells. In tissues, ARL-6 mRNA was most abundant in brain and kidney. While ARL-6 protein was predominantly cytosolic, its membrane association increased following exposure to GTP-gamma S, like many members of the ARF/ARL family. Using the yeast two-hybrid system, six molecules which interact with ARL-6 were identified including SEC61 beta, a subunit of the heterotrimeric protein conducting channel SEC61 beta, Co-immunoprecipitation of ARL-6 confirmed a stable association between ARL-6 and SEC61 beta in COS cells. These results demonstrate that ARL-6, a novel member of the ADP-ribosylation factor-like family, interacts with the SEC61 beta subunit, (C) 1999 Federation of European Biochemical Societies.
U2 - 10.1016/S0014-5793(99)01188-6
DO - 10.1016/S0014-5793(99)01188-6
M3 - Article
SN - 0014-5793
VL - 459
SP - 69
EP - 74
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -