A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61 beta subunit

Evan Ingley, J.H. Williams, C.E. Walker, S. Tsai, S. Colley, M.S. Sayer, Peta Tilbrook, M. Sarna, Jennifer Beaumont, Peter Klinken

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)

Abstract

We report here the isolation of a new member of the ADP-ribosylation factor (ARF)-like family (ARL-6) present in the J2E erythroleukemic cell line, but not its myeloid variants, Consistent with this lineage-restricted expression, ARL-6 mRNA increased with erythropoietin-induced maturation of J2E cells, and decreased with interleukin 6-induced differentiation of M1 monoblastoid cells. In tissues, ARL-6 mRNA was most abundant in brain and kidney. While ARL-6 protein was predominantly cytosolic, its membrane association increased following exposure to GTP-gamma S, like many members of the ARF/ARL family. Using the yeast two-hybrid system, six molecules which interact with ARL-6 were identified including SEC61 beta, a subunit of the heterotrimeric protein conducting channel SEC61 beta, Co-immunoprecipitation of ARL-6 confirmed a stable association between ARL-6 and SEC61 beta in COS cells. These results demonstrate that ARL-6, a novel member of the ADP-ribosylation factor-like family, interacts with the SEC61 beta subunit, (C) 1999 Federation of European Biochemical Societies.
Original languageEnglish
Pages (from-to)69-74
JournalFEBS Letters
Volume459
Issue number1
DOIs
Publication statusPublished - 1999

Fingerprint

Dive into the research topics of 'A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61 beta subunit'. Together they form a unique fingerprint.

Cite this