A new template for rhodopsin (vitamin A1 based) visual pigments

J. C. Partridge, W. J. De Grip

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

A new template curve for rhodopsin visual pigments is presented. The template was constructed from absorbance spectra of purified bovine rhodopsin with an abscissa transform of λmax/λ. Absorbance curves generated from the template are compared with absorbance spectra of visual pigments measured in situ in single cells by microspectrophotometry (msp). The template was found to agree well with msp data which included visual pigments with λmax values ranging from 450 to 570 nm. A cubic polynomial is given by which the new template may be fitted to the long wavelength limb of visual pigment data. A Chebyshev polynomial is also given which models the new template with a high degree of accuracy. This polynomial provides a convenient method for the computer generation of visual pigment absorbance curves.

Original languageEnglish
Pages (from-to)619-630
Number of pages12
JournalVision Research
Volume31
Issue number4
DOIs
Publication statusPublished - 1 Jan 1991
Externally publishedYes

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