A new pollination system in non-cleistogamous species of Viola results from nyctinastic (night-closing) petal movements - A mixed outcrossing-selfing strategy

Monika Kwiatkowska, Jerzy Bohdanowicz, Marlena Cubala, Aneta Stomka, Justyna Zabicka, Piotr Zabicki, Grzegorz Migdalek, Thomas Marcussen, Kevin Thiele, Elzbieta Kuta

Research output: Contribution to journalArticle

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Abstract

Viola banksii of the eastern Australian and Tasmanian sect. Erpetion produces exclusively chasmogamous flowers, unlike most other temperate taxa of Viola which additionally produce obligatory self-pollinated cleistogamous flowers. This study explored flower structure and nyctinastic flower movements (temporal flower closure), the correlation of the timing of nyctinastic movements with stigma receptivity, and self-compatibility of flowers. Petal movement in day/night cycles was documented by two cameras. Floral morphology, anther protuberance anatomy, stigma receptivity, and pollen tube growth were examined using scanning electron microscopy, fluorescent microscopy, histochemical and sectioning techniques. Diurnal petal movements that cause flowers to be open during the day and closed during the night were documented in V. banksii. Chasmogamous flower colour, fragrance, anterior petal venation, and the indurated green area at the base of the anterior petal are all likely to play roles for pollinator attraction. Unlike most other Viola species, the anther protuberances do not function as nectaries. The flowers offer pollinators no nectar reward. The short time of stigma receptivity (2-3 days) of individual flowers limits opportunities for insect visitation and cross-pollination. Night-closed flowers of V. banksii appear to facilitate self-pollination. Self-compatibility was confirmed by tracking the pollen tube growth from the stigma to the ovule in spontaneous and hand self-pollinated flowers. Over the floral phenological cycle, nyctinasty coincides in time with stigma receptivity and, hence, the ability of self-pollination. We discovered that the same chasmogamous flower of V. banksii can cross-pollinate while open during the day, but self-pollinate while closed during the night (i.e. acting as a cleistogamous-like flower). Several floral characters indicate that V. banksii exhibits a pollination deceit strategy. Such a pollination system has not been described elsewhere in Viola.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalFlora
Volume253
DOIs
Publication statusPublished - Apr 2019

Cite this

Kwiatkowska, Monika ; Bohdanowicz, Jerzy ; Cubala, Marlena ; Stomka, Aneta ; Zabicka, Justyna ; Zabicki, Piotr ; Migdalek, Grzegorz ; Marcussen, Thomas ; Thiele, Kevin ; Kuta, Elzbieta. / A new pollination system in non-cleistogamous species of Viola results from nyctinastic (night-closing) petal movements - A mixed outcrossing-selfing strategy. In: Flora. 2019 ; Vol. 253. pp. 1-9.
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abstract = "Viola banksii of the eastern Australian and Tasmanian sect. Erpetion produces exclusively chasmogamous flowers, unlike most other temperate taxa of Viola which additionally produce obligatory self-pollinated cleistogamous flowers. This study explored flower structure and nyctinastic flower movements (temporal flower closure), the correlation of the timing of nyctinastic movements with stigma receptivity, and self-compatibility of flowers. Petal movement in day/night cycles was documented by two cameras. Floral morphology, anther protuberance anatomy, stigma receptivity, and pollen tube growth were examined using scanning electron microscopy, fluorescent microscopy, histochemical and sectioning techniques. Diurnal petal movements that cause flowers to be open during the day and closed during the night were documented in V. banksii. Chasmogamous flower colour, fragrance, anterior petal venation, and the indurated green area at the base of the anterior petal are all likely to play roles for pollinator attraction. Unlike most other Viola species, the anther protuberances do not function as nectaries. The flowers offer pollinators no nectar reward. The short time of stigma receptivity (2-3 days) of individual flowers limits opportunities for insect visitation and cross-pollination. Night-closed flowers of V. banksii appear to facilitate self-pollination. Self-compatibility was confirmed by tracking the pollen tube growth from the stigma to the ovule in spontaneous and hand self-pollinated flowers. Over the floral phenological cycle, nyctinasty coincides in time with stigma receptivity and, hence, the ability of self-pollination. We discovered that the same chasmogamous flower of V. banksii can cross-pollinate while open during the day, but self-pollinate while closed during the night (i.e. acting as a cleistogamous-like flower). Several floral characters indicate that V. banksii exhibits a pollination deceit strategy. Such a pollination system has not been described elsewhere in Viola.",
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A new pollination system in non-cleistogamous species of Viola results from nyctinastic (night-closing) petal movements - A mixed outcrossing-selfing strategy. / Kwiatkowska, Monika; Bohdanowicz, Jerzy; Cubala, Marlena; Stomka, Aneta; Zabicka, Justyna; Zabicki, Piotr; Migdalek, Grzegorz; Marcussen, Thomas; Thiele, Kevin; Kuta, Elzbieta.

In: Flora, Vol. 253, 04.2019, p. 1-9.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A new pollination system in non-cleistogamous species of Viola results from nyctinastic (night-closing) petal movements - A mixed outcrossing-selfing strategy

AU - Kwiatkowska, Monika

AU - Bohdanowicz, Jerzy

AU - Cubala, Marlena

AU - Stomka, Aneta

AU - Zabicka, Justyna

AU - Zabicki, Piotr

AU - Migdalek, Grzegorz

AU - Marcussen, Thomas

AU - Thiele, Kevin

AU - Kuta, Elzbieta

PY - 2019/4

Y1 - 2019/4

N2 - Viola banksii of the eastern Australian and Tasmanian sect. Erpetion produces exclusively chasmogamous flowers, unlike most other temperate taxa of Viola which additionally produce obligatory self-pollinated cleistogamous flowers. This study explored flower structure and nyctinastic flower movements (temporal flower closure), the correlation of the timing of nyctinastic movements with stigma receptivity, and self-compatibility of flowers. Petal movement in day/night cycles was documented by two cameras. Floral morphology, anther protuberance anatomy, stigma receptivity, and pollen tube growth were examined using scanning electron microscopy, fluorescent microscopy, histochemical and sectioning techniques. Diurnal petal movements that cause flowers to be open during the day and closed during the night were documented in V. banksii. Chasmogamous flower colour, fragrance, anterior petal venation, and the indurated green area at the base of the anterior petal are all likely to play roles for pollinator attraction. Unlike most other Viola species, the anther protuberances do not function as nectaries. The flowers offer pollinators no nectar reward. The short time of stigma receptivity (2-3 days) of individual flowers limits opportunities for insect visitation and cross-pollination. Night-closed flowers of V. banksii appear to facilitate self-pollination. Self-compatibility was confirmed by tracking the pollen tube growth from the stigma to the ovule in spontaneous and hand self-pollinated flowers. Over the floral phenological cycle, nyctinasty coincides in time with stigma receptivity and, hence, the ability of self-pollination. We discovered that the same chasmogamous flower of V. banksii can cross-pollinate while open during the day, but self-pollinate while closed during the night (i.e. acting as a cleistogamous-like flower). Several floral characters indicate that V. banksii exhibits a pollination deceit strategy. Such a pollination system has not been described elsewhere in Viola.

AB - Viola banksii of the eastern Australian and Tasmanian sect. Erpetion produces exclusively chasmogamous flowers, unlike most other temperate taxa of Viola which additionally produce obligatory self-pollinated cleistogamous flowers. This study explored flower structure and nyctinastic flower movements (temporal flower closure), the correlation of the timing of nyctinastic movements with stigma receptivity, and self-compatibility of flowers. Petal movement in day/night cycles was documented by two cameras. Floral morphology, anther protuberance anatomy, stigma receptivity, and pollen tube growth were examined using scanning electron microscopy, fluorescent microscopy, histochemical and sectioning techniques. Diurnal petal movements that cause flowers to be open during the day and closed during the night were documented in V. banksii. Chasmogamous flower colour, fragrance, anterior petal venation, and the indurated green area at the base of the anterior petal are all likely to play roles for pollinator attraction. Unlike most other Viola species, the anther protuberances do not function as nectaries. The flowers offer pollinators no nectar reward. The short time of stigma receptivity (2-3 days) of individual flowers limits opportunities for insect visitation and cross-pollination. Night-closed flowers of V. banksii appear to facilitate self-pollination. Self-compatibility was confirmed by tracking the pollen tube growth from the stigma to the ovule in spontaneous and hand self-pollinated flowers. Over the floral phenological cycle, nyctinasty coincides in time with stigma receptivity and, hence, the ability of self-pollination. We discovered that the same chasmogamous flower of V. banksii can cross-pollinate while open during the day, but self-pollinate while closed during the night (i.e. acting as a cleistogamous-like flower). Several floral characters indicate that V. banksii exhibits a pollination deceit strategy. Such a pollination system has not been described elsewhere in Viola.

KW - Chasmogamous flower structure

KW - Nyctinasty

KW - Pollination system

KW - Self-compatibility

KW - Stigma receptivity

KW - Viola banksii

KW - SECTION ERPETION

KW - CLOSURE

KW - ECOLOGY

KW - MECHANISMS

KW - MORPHOLOGY

KW - EVOLUTION

KW - INSIGHTS

KW - SSP

U2 - 10.1016/j.flora.2019.01.007

DO - 10.1016/j.flora.2019.01.007

M3 - Article

VL - 253

SP - 1

EP - 9

JO - Flora

JF - Flora

SN - 0367-2530

ER -