@inproceedings{2662697889464dc7bcb04b67c69abc81,
title = "A new macro-imager based on Tpx3Cam optical camera for PLIM applications",
abstract = "The recently designed Tpx3Cam camera based PLIM (Phosphorescence Lifetime IMaging) macro-imager was tested using an array of phosphorescent chemical and biological samples. A series of sensor materials prepared by incorporating the phosphorescent O2-sensitive dye, PtBP, into five polymers with different O2 permeability were imaged along with several commercial and non-commercial sensors based on PtBP and PtOEPK dyes. The PLIM images showed good lifetime contrast between the different materials, and phosphorescence lifetime values obtained were consistent with those measured by alternative methods. A panel of live tissues samples stained with PtBP based nanoparticle probe were also prepared and imaged under resting conditions and upon inhibition of respiration. The macro-imager showed promising results as a tool for PLIM of O2 in chemical and biological samples.",
keywords = "Fluorescence and Phosphorescence Lifetime Imaging (FLIM and PLIM), Oxygen Sensing and Imaging, Time Correlated Single Photon Counting (TCSPC), Tpx3Cam camera",
author = "Rajannya Sen and Alexander Zhdanov and Hirvonen, {Liisa M.} and Peter Svihra and Stefan Andersson-Engels and Andrei Nomerotski and Dmitri Papkovsky",
year = "2020",
doi = "10.1117/12.2555387",
language = "English",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "SPIE - International Society for Optical Engineering",
editor = "Jurgen Popp and Csilla Gergely",
booktitle = "Biomedical Spectroscopy, Microscopy, and Imaging",
note = "Biomedical Spectroscopy, Microscopy, and Imaging 2020 ; Conference date: 06-04-2020 Through 10-04-2020",
}