A new macro-imager based on Tpx3Cam optical camera for PLIM applications

Rajannya Sen, Alexander Zhdanov, Liisa M. Hirvonen, Peter Svihra, Stefan Andersson-Engels, Andrei Nomerotski, Dmitri Papkovsky

Research output: Chapter in Book/Conference paperConference paper

1 Citation (Scopus)

Abstract

The recently designed Tpx3Cam camera based PLIM (Phosphorescence Lifetime IMaging) macro-imager was tested using an array of phosphorescent chemical and biological samples. A series of sensor materials prepared by incorporating the phosphorescent O2-sensitive dye, PtBP, into five polymers with different O2 permeability were imaged along with several commercial and non-commercial sensors based on PtBP and PtOEPK dyes. The PLIM images showed good lifetime contrast between the different materials, and phosphorescence lifetime values obtained were consistent with those measured by alternative methods. A panel of live tissues samples stained with PtBP based nanoparticle probe were also prepared and imaged under resting conditions and upon inhibition of respiration. The macro-imager showed promising results as a tool for PLIM of O2 in chemical and biological samples.

Original languageEnglish
Title of host publicationBiomedical Spectroscopy, Microscopy, and Imaging
EditorsJurgen Popp, Csilla Gergely
PublisherSPIE - International Society for Optical Engineering
ISBN (Electronic)9781510634909
DOIs
Publication statusPublished - 2020
EventBiomedical Spectroscopy, Microscopy, and Imaging 2020 - Virtual, Online, France
Duration: 6 Apr 202010 Apr 2020

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume11359
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceBiomedical Spectroscopy, Microscopy, and Imaging 2020
CountryFrance
CityVirtual, Online
Period6/04/2010/04/20

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