A multi-step peptidolytic cascade for amino acid recovery in chloroplasts

Pedro F. Teixeira; Beata Kmiec; Rui M M Branca; Monika W. Murcha; Anna Byzia; Aneta Ivanova; James Whelan; Marcin Drag; Janne Lehtiö; Elzbieta Glaser

doi:10.1038/nchembio.2227

A multi-step peptidolytic cascade for amino acid recovery in chloroplasts

Pedro F. Teixeira, Beata Kmiec, Rui M M Branca, Monika W. Murcha, Anna Byzia, Aneta Ivanova, James Whelan, Marcin Drag, Janne Lehtiö, Elzbieta Glaser

ARC Centre for Plant Energy Biology

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Plastids (including chloroplasts) are subcellular sites for a plethora of proteolytic reactions, required in functions ranging from protein biogenesis to quality control. Here we show that peptides generated from pre-protein maturation within chloroplasts of Arabidopsis thaliana are degraded to amino acids by a multi-step peptidolytic cascade consisting of oligopeptidases and aminopeptidases, effectively allowing the recovery of single amino acids within these organelles.

Original language	English
Pages (from-to)	15-17
Number of pages	3
Journal	Nature Chemical Biology
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/nchembio.2227
Publication status	Published - 1 Jan 2017

Access to Document

10.1038/nchembio.2227

Cite this

@article{efd1f084ee1b4951a81036c70894e9b9,

title = "A multi-step peptidolytic cascade for amino acid recovery in chloroplasts",

abstract = "Plastids (including chloroplasts) are subcellular sites for a plethora of proteolytic reactions, required in functions ranging from protein biogenesis to quality control. Here we show that peptides generated from pre-protein maturation within chloroplasts of Arabidopsis thaliana are degraded to amino acids by a multi-step peptidolytic cascade consisting of oligopeptidases and aminopeptidases, effectively allowing the recovery of single amino acids within these organelles.",

author = "Teixeira, {Pedro F.} and Beata Kmiec and Branca, {Rui M M} and Murcha, {Monika W.} and Anna Byzia and Aneta Ivanova and James Whelan and Marcin Drag and Janne Lehti{\"o} and Elzbieta Glaser",

year = "2017",

month = jan,

day = "1",

doi = "10.1038/nchembio.2227",

language = "English",

volume = "13",

pages = "15--17",

journal = "Nature Chemical Biology",

issn = "1552-4450",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - A multi-step peptidolytic cascade for amino acid recovery in chloroplasts

AU - Teixeira, Pedro F.

AU - Kmiec, Beata

AU - Branca, Rui M M

AU - Murcha, Monika W.

AU - Byzia, Anna

AU - Ivanova, Aneta

AU - Whelan, James

AU - Drag, Marcin

AU - Lehtiö, Janne

AU - Glaser, Elzbieta

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Plastids (including chloroplasts) are subcellular sites for a plethora of proteolytic reactions, required in functions ranging from protein biogenesis to quality control. Here we show that peptides generated from pre-protein maturation within chloroplasts of Arabidopsis thaliana are degraded to amino acids by a multi-step peptidolytic cascade consisting of oligopeptidases and aminopeptidases, effectively allowing the recovery of single amino acids within these organelles.

AB - Plastids (including chloroplasts) are subcellular sites for a plethora of proteolytic reactions, required in functions ranging from protein biogenesis to quality control. Here we show that peptides generated from pre-protein maturation within chloroplasts of Arabidopsis thaliana are degraded to amino acids by a multi-step peptidolytic cascade consisting of oligopeptidases and aminopeptidases, effectively allowing the recovery of single amino acids within these organelles.

UR - http://www.scopus.com/inward/record.url?scp=84994613718&partnerID=8YFLogxK

U2 - 10.1038/nchembio.2227

DO - 10.1038/nchembio.2227

M3 - Article

C2 - 27820795

AN - SCOPUS:84994613718

SN - 1552-4450

VL - 13

SP - 15

EP - 17

JO - Nature Chemical Biology

JF - Nature Chemical Biology

IS - 1

ER -

A multi-step peptidolytic cascade for amino acid recovery in chloroplasts

Abstract

Access to Document

Other files and links

Fingerprint

Functional Analysis of Novel Mitochondrial Outer Membrane Proteins in Arabidopsis

A mitochondrial prolyl aminopeptidase PAP2 releases N‐terminal proline and regulates proline homeostasis during stress response

Cite this

A multi-step peptidolytic cascade for amino acid recovery in chloroplasts

Abstract

Access to Document

Other files and links

Fingerprint

Projects

Functional Analysis of Novel Mitochondrial Outer Membrane Proteins in Arabidopsis

Research output

A mitochondrial prolyl aminopeptidase PAP2 releases N‐terminal proline and regulates proline homeostasis during stress response

Cite this