A model of sodium channels

T. Vora; Ben Corry; S-H. Chung

doi:10.1016/j.bbamem.2004.11.011

A model of sodium channels

T. Vora, Ben Corry, S-H. Chung

School of Molecular Sciences

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

We have explored the permeation and blockage of ions in sodium channels, relating the channel structure to function using electrostatic profiles and Brownian dynamics simulations. The model used resembles the KcsA potassium channel with an added external vestibule and a shorter selectivity filter. The electrostatic energy landscape seen by permeating ions is determined by solving Poisson's equation. The two charged amino acid rings of Glu-Glu-Asp-Asp (EEDD) and Asp-Glu-Lys-Ala (DEKA) around the selectivity filter region are seen to play a crucial role in making the channel sodium selective, and strongly binding calcium ions such that they block the channel. Our model closely reproduces a range of experimental data including the current-voltage curves, current-concentration curves and blockage of monovalent ions by divalent ions. (C) 2004 Elsevier B.V All rights reserved.

Original language	English
Pages (from-to)	106-116
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1668
Issue number	1
DOIs	https://doi.org/10.1016/j.bbamem.2004.11.011
Publication status	Published - 2005

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10.1016/j.bbamem.2004.11.011

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AU - Corry, Ben

AU - Chung, S-H.

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AB - We have explored the permeation and blockage of ions in sodium channels, relating the channel structure to function using electrostatic profiles and Brownian dynamics simulations. The model used resembles the KcsA potassium channel with an added external vestibule and a shorter selectivity filter. The electrostatic energy landscape seen by permeating ions is determined by solving Poisson's equation. The two charged amino acid rings of Glu-Glu-Asp-Asp (EEDD) and Asp-Glu-Lys-Ala (DEKA) around the selectivity filter region are seen to play a crucial role in making the channel sodium selective, and strongly binding calcium ions such that they block the channel. Our model closely reproduces a range of experimental data including the current-voltage curves, current-concentration curves and blockage of monovalent ions by divalent ions. (C) 2004 Elsevier B.V All rights reserved.

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EP - 116

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

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A model of sodium channels

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