A MECHANISM OF CHLOROTOLURON RESISTANCE IN LOLIUM-RIGIDUM

MWM Burnet, BR Loveys, JAM Holtum, Stephen Powles

    Research output: Contribution to journalArticle

    48 Citations (Scopus)

    Abstract

    Many biotypes of Lolium rigidum Gaud. (annual ryegrass) have developed resistance to herbicides; however, few have developed resistance to phenylurea herbicides. Two biotypes with different histories of herbicide selection pressure were six to eight times less sensitive to the phenylurea herbicide, chlorotoluron, than a susceptible biotype. Resistance was not due to differences in the herbicide target site as oxygen evolution by thylakoids isolated from resistant and susceptible biotypes was similarly inhibited by diuron and chlorotoluron. There was no difference in the uptake and distribution of chlorotoluron into resistant and susceptible plants. There was a twofold greater rate of chlorotoluron detoxification in resistant plants with N-demethylation being a major detoxification reaction. Resistant plants treated with a 3-h pulse of 120 muM chlorotoluron recovered net carbon fixation after 42 h, half the time taken by susceptible plants. The mixed-function oxidase inhibitor 1-aminobenzotriazole (70 muM) intensified the effects of chlorotoluron in resistant plants when applied in combination with the herbicide for 7 d. 1-Aminobenzotriazole also inhibited the metabolism of chlorotoluron in both resistant and susceptible plants. The cytochrome P-450 inhibitor, piperonyl butoxide piperonyl butoxide, interacted with chlorotoluron when applied to plants growing in soil. Chlorotoluron applied with reduced plant dry weight to a greater extent than chlorotoluron alone. It appears, therefore, that enhanced detoxification is the major mechanism of resistance to chlorotoluron in the resistant biotypes studied.
    Original languageEnglish
    Pages (from-to)182-189
    JournalPlanta
    Volume190
    Publication statusPublished - 1993

    Fingerprint

    chlorotoluron
    Lolium rigidum
    Lolium
    Herbicides
    biotypes
    phenylurea herbicides
    Piperonyl Butoxide
    piperonyl butoxide
    Chrysanthemum morifolium
    herbicides
    chlortoluron
    Herbicide Resistance
    Diuron
    mixed function oxidase
    Carbon Cycle
    diuron
    Thylakoids
    herbicide resistance
    Calvin cycle
    Mixed Function Oxygenases

    Cite this

    Burnet, MWM., Loveys, BR., Holtum, JAM., & Powles, S. (1993). A MECHANISM OF CHLOROTOLURON RESISTANCE IN LOLIUM-RIGIDUM. Planta, 190, 182-189.
    Burnet, MWM ; Loveys, BR ; Holtum, JAM ; Powles, Stephen. / A MECHANISM OF CHLOROTOLURON RESISTANCE IN LOLIUM-RIGIDUM. In: Planta. 1993 ; Vol. 190. pp. 182-189.
    @article{bb266855358f4a1797ca85937cd732e7,
    title = "A MECHANISM OF CHLOROTOLURON RESISTANCE IN LOLIUM-RIGIDUM",
    abstract = "Many biotypes of Lolium rigidum Gaud. (annual ryegrass) have developed resistance to herbicides; however, few have developed resistance to phenylurea herbicides. Two biotypes with different histories of herbicide selection pressure were six to eight times less sensitive to the phenylurea herbicide, chlorotoluron, than a susceptible biotype. Resistance was not due to differences in the herbicide target site as oxygen evolution by thylakoids isolated from resistant and susceptible biotypes was similarly inhibited by diuron and chlorotoluron. There was no difference in the uptake and distribution of chlorotoluron into resistant and susceptible plants. There was a twofold greater rate of chlorotoluron detoxification in resistant plants with N-demethylation being a major detoxification reaction. Resistant plants treated with a 3-h pulse of 120 muM chlorotoluron recovered net carbon fixation after 42 h, half the time taken by susceptible plants. The mixed-function oxidase inhibitor 1-aminobenzotriazole (70 muM) intensified the effects of chlorotoluron in resistant plants when applied in combination with the herbicide for 7 d. 1-Aminobenzotriazole also inhibited the metabolism of chlorotoluron in both resistant and susceptible plants. The cytochrome P-450 inhibitor, piperonyl butoxide piperonyl butoxide, interacted with chlorotoluron when applied to plants growing in soil. Chlorotoluron applied with reduced plant dry weight to a greater extent than chlorotoluron alone. It appears, therefore, that enhanced detoxification is the major mechanism of resistance to chlorotoluron in the resistant biotypes studied.",
    author = "MWM Burnet and BR Loveys and JAM Holtum and Stephen Powles",
    year = "1993",
    language = "English",
    volume = "190",
    pages = "182--189",
    journal = "Planta - an international journal of plant biology",
    issn = "0032-0935",
    publisher = "Springer",

    }

    Burnet, MWM, Loveys, BR, Holtum, JAM & Powles, S 1993, 'A MECHANISM OF CHLOROTOLURON RESISTANCE IN LOLIUM-RIGIDUM' Planta, vol. 190, pp. 182-189.

    A MECHANISM OF CHLOROTOLURON RESISTANCE IN LOLIUM-RIGIDUM. / Burnet, MWM; Loveys, BR; Holtum, JAM; Powles, Stephen.

    In: Planta, Vol. 190, 1993, p. 182-189.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - A MECHANISM OF CHLOROTOLURON RESISTANCE IN LOLIUM-RIGIDUM

    AU - Burnet, MWM

    AU - Loveys, BR

    AU - Holtum, JAM

    AU - Powles, Stephen

    PY - 1993

    Y1 - 1993

    N2 - Many biotypes of Lolium rigidum Gaud. (annual ryegrass) have developed resistance to herbicides; however, few have developed resistance to phenylurea herbicides. Two biotypes with different histories of herbicide selection pressure were six to eight times less sensitive to the phenylurea herbicide, chlorotoluron, than a susceptible biotype. Resistance was not due to differences in the herbicide target site as oxygen evolution by thylakoids isolated from resistant and susceptible biotypes was similarly inhibited by diuron and chlorotoluron. There was no difference in the uptake and distribution of chlorotoluron into resistant and susceptible plants. There was a twofold greater rate of chlorotoluron detoxification in resistant plants with N-demethylation being a major detoxification reaction. Resistant plants treated with a 3-h pulse of 120 muM chlorotoluron recovered net carbon fixation after 42 h, half the time taken by susceptible plants. The mixed-function oxidase inhibitor 1-aminobenzotriazole (70 muM) intensified the effects of chlorotoluron in resistant plants when applied in combination with the herbicide for 7 d. 1-Aminobenzotriazole also inhibited the metabolism of chlorotoluron in both resistant and susceptible plants. The cytochrome P-450 inhibitor, piperonyl butoxide piperonyl butoxide, interacted with chlorotoluron when applied to plants growing in soil. Chlorotoluron applied with reduced plant dry weight to a greater extent than chlorotoluron alone. It appears, therefore, that enhanced detoxification is the major mechanism of resistance to chlorotoluron in the resistant biotypes studied.

    AB - Many biotypes of Lolium rigidum Gaud. (annual ryegrass) have developed resistance to herbicides; however, few have developed resistance to phenylurea herbicides. Two biotypes with different histories of herbicide selection pressure were six to eight times less sensitive to the phenylurea herbicide, chlorotoluron, than a susceptible biotype. Resistance was not due to differences in the herbicide target site as oxygen evolution by thylakoids isolated from resistant and susceptible biotypes was similarly inhibited by diuron and chlorotoluron. There was no difference in the uptake and distribution of chlorotoluron into resistant and susceptible plants. There was a twofold greater rate of chlorotoluron detoxification in resistant plants with N-demethylation being a major detoxification reaction. Resistant plants treated with a 3-h pulse of 120 muM chlorotoluron recovered net carbon fixation after 42 h, half the time taken by susceptible plants. The mixed-function oxidase inhibitor 1-aminobenzotriazole (70 muM) intensified the effects of chlorotoluron in resistant plants when applied in combination with the herbicide for 7 d. 1-Aminobenzotriazole also inhibited the metabolism of chlorotoluron in both resistant and susceptible plants. The cytochrome P-450 inhibitor, piperonyl butoxide piperonyl butoxide, interacted with chlorotoluron when applied to plants growing in soil. Chlorotoluron applied with reduced plant dry weight to a greater extent than chlorotoluron alone. It appears, therefore, that enhanced detoxification is the major mechanism of resistance to chlorotoluron in the resistant biotypes studied.

    M3 - Article

    VL - 190

    SP - 182

    EP - 189

    JO - Planta - an international journal of plant biology

    JF - Planta - an international journal of plant biology

    SN - 0032-0935

    ER -

    Burnet MWM, Loveys BR, Holtum JAM, Powles S. A MECHANISM OF CHLOROTOLURON RESISTANCE IN LOLIUM-RIGIDUM. Planta. 1993;190:182-189.