A major locus involved in the formation of the radial oxygen loss barrier in adventitious roots of teosinte Zea nicaraguensis is located on the short-arm of chromosome 3

Kohtaro Watanabe, Hirokazu Takahashi, Saori Sato, Shunsaku Nishiuchi, Fumie Omori, Al Imran Malik, Timothy David Colmer, Yoshiro Mano, Mikio Nakazono

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A radial oxygen loss (ROL) barrier in roots of waterlogging-tolerant plants promotes oxygen movement via aerenchyma to the root tip, and impedes soil phytotoxin entry. The molecular mechanism and genetic regulation of ROL barrier formation are largely unknown. Zea nicaraguensis, a waterlogging-tolerant wild relative of maize (Zea mays ssp. mays), forms a tight ROL barrier in its roots when waterlogged. We used Z. nicaraguensis chromosome segment introgression lines (ILs) in maize (inbred line Mi29) to elucidate the chromosomal region involved in regulating root ROL barrier formation. A segment of the short-arm of chromosome 3 of Z. nicaraguensis conferred ROL barrier formation in the genetic background of maize. This chromosome segment also decreased apoplastic solute permeability across the hypodermis/exodermis. However, the IL and maize were similar for suberin staining in the hypodermis/exodermis at 40 mm and further behind the root tip. Z. nicaraguensis contained suberin in the hypodermis/exodermis at 20 mm and lignin at the epidermis. The IL with ROL barrier, however, did not contain lignin in the epidermis. Discovery of the Z. nicaraguensis chromosomal region responsible for root ROL barrier formation has improved knowledge of this trait and is an important step towards improvement of waterlogging tolerance in maize. 

Original languageEnglish
Pages (from-to)304-316
Number of pages13
JournalPlant Cell and Environment
Volume40
Issue number2
DOIs
Publication statusPublished - 1 Feb 2017

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Zea nicaraguensis
Chromosomes, Human, Pair 3
Zea
adventitious roots
Zea mays
Oxygen
chromosomes
oxygen
loci
Subcutaneous Tissue
flooded conditions
corn
introgression
suberin
Meristem
Lignin
epidermis (plant)
root tips
Epidermis
lignin

Cite this

@article{07c69da1fbae4474bac6ffab4ee1281c,
title = "A major locus involved in the formation of the radial oxygen loss barrier in adventitious roots of teosinte Zea nicaraguensis is located on the short-arm of chromosome 3",
abstract = "A radial oxygen loss (ROL) barrier in roots of waterlogging-tolerant plants promotes oxygen movement via aerenchyma to the root tip, and impedes soil phytotoxin entry. The molecular mechanism and genetic regulation of ROL barrier formation are largely unknown. Zea nicaraguensis, a waterlogging-tolerant wild relative of maize (Zea mays ssp. mays), forms a tight ROL barrier in its roots when waterlogged. We used Z. nicaraguensis chromosome segment introgression lines (ILs) in maize (inbred line Mi29) to elucidate the chromosomal region involved in regulating root ROL barrier formation. A segment of the short-arm of chromosome 3 of Z. nicaraguensis conferred ROL barrier formation in the genetic background of maize. This chromosome segment also decreased apoplastic solute permeability across the hypodermis/exodermis. However, the IL and maize were similar for suberin staining in the hypodermis/exodermis at 40 mm and further behind the root tip. Z. nicaraguensis contained suberin in the hypodermis/exodermis at 20 mm and lignin at the epidermis. The IL with ROL barrier, however, did not contain lignin in the epidermis. Discovery of the Z. nicaraguensis chromosomal region responsible for root ROL barrier formation has improved knowledge of this trait and is an important step towards improvement of waterlogging tolerance in maize. ",
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author = "Kohtaro Watanabe and Hirokazu Takahashi and Saori Sato and Shunsaku Nishiuchi and Fumie Omori and Malik, {Al Imran} and Colmer, {Timothy David} and Yoshiro Mano and Mikio Nakazono",
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month = "2",
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volume = "40",
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issn = "0140-7791",
publisher = "John Wiley & Sons",
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A major locus involved in the formation of the radial oxygen loss barrier in adventitious roots of teosinte Zea nicaraguensis is located on the short-arm of chromosome 3. / Watanabe, Kohtaro; Takahashi, Hirokazu; Sato, Saori; Nishiuchi, Shunsaku; Omori, Fumie; Malik, Al Imran; Colmer, Timothy David; Mano, Yoshiro; Nakazono, Mikio.

In: Plant Cell and Environment, Vol. 40, No. 2, 01.02.2017, p. 304-316.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A major locus involved in the formation of the radial oxygen loss barrier in adventitious roots of teosinte Zea nicaraguensis is located on the short-arm of chromosome 3

AU - Watanabe, Kohtaro

AU - Takahashi, Hirokazu

AU - Sato, Saori

AU - Nishiuchi, Shunsaku

AU - Omori, Fumie

AU - Malik, Al Imran

AU - Colmer, Timothy David

AU - Mano, Yoshiro

AU - Nakazono, Mikio

PY - 2017/2/1

Y1 - 2017/2/1

N2 - A radial oxygen loss (ROL) barrier in roots of waterlogging-tolerant plants promotes oxygen movement via aerenchyma to the root tip, and impedes soil phytotoxin entry. The molecular mechanism and genetic regulation of ROL barrier formation are largely unknown. Zea nicaraguensis, a waterlogging-tolerant wild relative of maize (Zea mays ssp. mays), forms a tight ROL barrier in its roots when waterlogged. We used Z. nicaraguensis chromosome segment introgression lines (ILs) in maize (inbred line Mi29) to elucidate the chromosomal region involved in regulating root ROL barrier formation. A segment of the short-arm of chromosome 3 of Z. nicaraguensis conferred ROL barrier formation in the genetic background of maize. This chromosome segment also decreased apoplastic solute permeability across the hypodermis/exodermis. However, the IL and maize were similar for suberin staining in the hypodermis/exodermis at 40 mm and further behind the root tip. Z. nicaraguensis contained suberin in the hypodermis/exodermis at 20 mm and lignin at the epidermis. The IL with ROL barrier, however, did not contain lignin in the epidermis. Discovery of the Z. nicaraguensis chromosomal region responsible for root ROL barrier formation has improved knowledge of this trait and is an important step towards improvement of waterlogging tolerance in maize. 

AB - A radial oxygen loss (ROL) barrier in roots of waterlogging-tolerant plants promotes oxygen movement via aerenchyma to the root tip, and impedes soil phytotoxin entry. The molecular mechanism and genetic regulation of ROL barrier formation are largely unknown. Zea nicaraguensis, a waterlogging-tolerant wild relative of maize (Zea mays ssp. mays), forms a tight ROL barrier in its roots when waterlogged. We used Z. nicaraguensis chromosome segment introgression lines (ILs) in maize (inbred line Mi29) to elucidate the chromosomal region involved in regulating root ROL barrier formation. A segment of the short-arm of chromosome 3 of Z. nicaraguensis conferred ROL barrier formation in the genetic background of maize. This chromosome segment also decreased apoplastic solute permeability across the hypodermis/exodermis. However, the IL and maize were similar for suberin staining in the hypodermis/exodermis at 40 mm and further behind the root tip. Z. nicaraguensis contained suberin in the hypodermis/exodermis at 20 mm and lignin at the epidermis. The IL with ROL barrier, however, did not contain lignin in the epidermis. Discovery of the Z. nicaraguensis chromosomal region responsible for root ROL barrier formation has improved knowledge of this trait and is an important step towards improvement of waterlogging tolerance in maize. 

KW - aerenchyma

KW - crop flooding tolerance

KW - introgression lines

KW - lignin

KW - maize

KW - suberin

KW - waterlogging tolerance

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U2 - 10.1111/pce.12849

DO - 10.1111/pce.12849

M3 - Article

VL - 40

SP - 304

EP - 316

JO - Plant, Cell and Environment.

JF - Plant, Cell and Environment.

SN - 0140-7791

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