A major and stable QTL for wheat spikelet number per spike validated in different genetic backgrounds

Pu yang DING, Zi qiang MO, Hua ping TANG, Yang MU, Mei DENG, Qian tao JIANG, Ya xi LIU, Guang deng CHEN, Guo yue CHEN, Ji rui WANG, Wei LI, Peng fei QI, Yun feng JIANG, Hou yang KANG, Gui jun YAN, Yu ming WEI, You liang ZHENG, Xiu jin LAN, Jian MA

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The spikelet number per spike (SNS) contributes greatly to grain yield in wheat. Identifying various genes that control wheat SNS is vital for yield improvement. This study used a recombinant inbred line population genotyped by the Wheat55K single-nucleotide polymorphism array to identify two major and stably expressed quantitative trait loci (QTLs) for SNS. One of them (QSns.sau-2SY-2D.1) was reported previously, while the other (QSns.sau-2SY-7A) was newly detected and further analyzed in this study. QSns.sau-2SY-7A had a high LOD value ranging from 4.46 to 16.00 and explained 10.21–40.78% of the phenotypic variances. QSns.sau-2SY-7A was flanked by the markers AX-110518554 and AX-110094527 in a 4.75-cM interval on chromosome arm 7AL. The contributions and interactions of both major QTLs were further analyzed and discussed. The effect of QSns.sau-2SY-7A was successfully validated by developing a tightly linked kompetitive allele specific PCR marker in an F2:3 population and a panel of 101 high-generation breeding wheat lines. Furthermore, several genes including the previously reported WHEAT ORTHOLOG OF APO1 (WAPO1), an ortholog of the rice gene ABERRANT PANICLE ORGANIZATION 1 (APO1) related to SNS, were predicted in the interval of QSns.sau-2SY-7A. In summary, these results revealed the genetic basis of the multi-spikelet genotype of wheat line 20828 and will facilitate subsequent fine mapping and breeding utilization of the major QTLs.

Original languageEnglish
Pages (from-to)1551-1562
Number of pages12
JournalJournal of Integrative Agriculture
Volume21
Issue number6
DOIs
Publication statusPublished - Jun 2022

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