A locus affecting immunoglobulin isotype selection (Igis1) maps to the MHC region in C57BL, BALB/c and NOD mice

V.B. Matthews, Grant Morahan, Patricia Price

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have previously shown that 1121 mice with 1310 or BALB genetic backgrounds have higher basal levels of IgG2a than H2(k) and H2(d) congenic strains and, hence, have low IgG1/IgG2a ratios, which is consistent with a T1 cytokine milieu. The phenotypic marker of the high IgG2a levels, denoted immunoglobulin isotype-1 (Igis1) was provisionally mapped telomeric of IEbeta using MHC recombinant mice. In addition, data from B10.A(2R), B10.A(1R) and B10.A(18R) mice indicated that Igis1 may lie in a 27 kb region between G7b (Sm-X5) and G7c. In the present study we confirm that Igis1 is in the H2 region using BALB and B 10 congenic F2 mice. H2(bb) F2 mice had higher IgG2a levels than the H2(dd) parental strains. H2(bd) F1 and F2 mice on the B 10 background produced IgG2a levels comparable with the H2(bb) parental strain, indicating that the b allele was dominant. In contrast, the H2bd F1 and F2 mice on the BALB background produced IgG2a levels between those of the H2(bb) and H2(dd) parental strains, indicating codominance of the b and d alleles. This suggests that a background gene influences regulation of IgG2a levels by Igis1. Non-obese diabetic (NOD) mice (K(d)IA(g7)IE(nu11)D(b)), which can develop type I diabetes, had higher levels of IgG2a than NOD-H2(d) congenic mice. Thus, Igis1 affects isotype selection in the presence of non-MHC diabetes genes. As type 1 diabetes is associated with T1 responses, Igis1 may affect susceptibility to this condition.
Original languageEnglish
Pages (from-to)576-582
JournalImmunology and Cell Biology
Volume79
Issue numberN/A
DOIs
Publication statusPublished - 2001

Fingerprint

Inbred NOD Mouse
Immunoglobulin Isotypes
Congenic Mice
Type 1 Diabetes Mellitus
Alleles
Genes
Immunoglobulin G
Cytokines

Cite this

@article{fb2e43a6587d48b18e18c24b3399c677,
title = "A locus affecting immunoglobulin isotype selection (Igis1) maps to the MHC region in C57BL, BALB/c and NOD mice",
abstract = "We have previously shown that 1121 mice with 1310 or BALB genetic backgrounds have higher basal levels of IgG2a than H2(k) and H2(d) congenic strains and, hence, have low IgG1/IgG2a ratios, which is consistent with a T1 cytokine milieu. The phenotypic marker of the high IgG2a levels, denoted immunoglobulin isotype-1 (Igis1) was provisionally mapped telomeric of IEbeta using MHC recombinant mice. In addition, data from B10.A(2R), B10.A(1R) and B10.A(18R) mice indicated that Igis1 may lie in a 27 kb region between G7b (Sm-X5) and G7c. In the present study we confirm that Igis1 is in the H2 region using BALB and B 10 congenic F2 mice. H2(bb) F2 mice had higher IgG2a levels than the H2(dd) parental strains. H2(bd) F1 and F2 mice on the B 10 background produced IgG2a levels comparable with the H2(bb) parental strain, indicating that the b allele was dominant. In contrast, the H2bd F1 and F2 mice on the BALB background produced IgG2a levels between those of the H2(bb) and H2(dd) parental strains, indicating codominance of the b and d alleles. This suggests that a background gene influences regulation of IgG2a levels by Igis1. Non-obese diabetic (NOD) mice (K(d)IA(g7)IE(nu11)D(b)), which can develop type I diabetes, had higher levels of IgG2a than NOD-H2(d) congenic mice. Thus, Igis1 affects isotype selection in the presence of non-MHC diabetes genes. As type 1 diabetes is associated with T1 responses, Igis1 may affect susceptibility to this condition.",
author = "V.B. Matthews and Grant Morahan and Patricia Price",
year = "2001",
doi = "10.1046/j.1440-1711.2001.01039.x",
language = "English",
volume = "79",
pages = "576--582",
journal = "Immunology & Cell Biology",
issn = "0818-9641",
publisher = "Nature Publishing Group - Macmillan Publishers",
number = "N/A",

}

A locus affecting immunoglobulin isotype selection (Igis1) maps to the MHC region in C57BL, BALB/c and NOD mice. / Matthews, V.B.; Morahan, Grant; Price, Patricia.

In: Immunology and Cell Biology, Vol. 79, No. N/A, 2001, p. 576-582.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A locus affecting immunoglobulin isotype selection (Igis1) maps to the MHC region in C57BL, BALB/c and NOD mice

AU - Matthews, V.B.

AU - Morahan, Grant

AU - Price, Patricia

PY - 2001

Y1 - 2001

N2 - We have previously shown that 1121 mice with 1310 or BALB genetic backgrounds have higher basal levels of IgG2a than H2(k) and H2(d) congenic strains and, hence, have low IgG1/IgG2a ratios, which is consistent with a T1 cytokine milieu. The phenotypic marker of the high IgG2a levels, denoted immunoglobulin isotype-1 (Igis1) was provisionally mapped telomeric of IEbeta using MHC recombinant mice. In addition, data from B10.A(2R), B10.A(1R) and B10.A(18R) mice indicated that Igis1 may lie in a 27 kb region between G7b (Sm-X5) and G7c. In the present study we confirm that Igis1 is in the H2 region using BALB and B 10 congenic F2 mice. H2(bb) F2 mice had higher IgG2a levels than the H2(dd) parental strains. H2(bd) F1 and F2 mice on the B 10 background produced IgG2a levels comparable with the H2(bb) parental strain, indicating that the b allele was dominant. In contrast, the H2bd F1 and F2 mice on the BALB background produced IgG2a levels between those of the H2(bb) and H2(dd) parental strains, indicating codominance of the b and d alleles. This suggests that a background gene influences regulation of IgG2a levels by Igis1. Non-obese diabetic (NOD) mice (K(d)IA(g7)IE(nu11)D(b)), which can develop type I diabetes, had higher levels of IgG2a than NOD-H2(d) congenic mice. Thus, Igis1 affects isotype selection in the presence of non-MHC diabetes genes. As type 1 diabetes is associated with T1 responses, Igis1 may affect susceptibility to this condition.

AB - We have previously shown that 1121 mice with 1310 or BALB genetic backgrounds have higher basal levels of IgG2a than H2(k) and H2(d) congenic strains and, hence, have low IgG1/IgG2a ratios, which is consistent with a T1 cytokine milieu. The phenotypic marker of the high IgG2a levels, denoted immunoglobulin isotype-1 (Igis1) was provisionally mapped telomeric of IEbeta using MHC recombinant mice. In addition, data from B10.A(2R), B10.A(1R) and B10.A(18R) mice indicated that Igis1 may lie in a 27 kb region between G7b (Sm-X5) and G7c. In the present study we confirm that Igis1 is in the H2 region using BALB and B 10 congenic F2 mice. H2(bb) F2 mice had higher IgG2a levels than the H2(dd) parental strains. H2(bd) F1 and F2 mice on the B 10 background produced IgG2a levels comparable with the H2(bb) parental strain, indicating that the b allele was dominant. In contrast, the H2bd F1 and F2 mice on the BALB background produced IgG2a levels between those of the H2(bb) and H2(dd) parental strains, indicating codominance of the b and d alleles. This suggests that a background gene influences regulation of IgG2a levels by Igis1. Non-obese diabetic (NOD) mice (K(d)IA(g7)IE(nu11)D(b)), which can develop type I diabetes, had higher levels of IgG2a than NOD-H2(d) congenic mice. Thus, Igis1 affects isotype selection in the presence of non-MHC diabetes genes. As type 1 diabetes is associated with T1 responses, Igis1 may affect susceptibility to this condition.

U2 - 10.1046/j.1440-1711.2001.01039.x

DO - 10.1046/j.1440-1711.2001.01039.x

M3 - Article

VL - 79

SP - 576

EP - 582

JO - Immunology & Cell Biology

JF - Immunology & Cell Biology

SN - 0818-9641

IS - N/A

ER -