A Link between RNA Metabolism and Silencing Affecting Arabidopsis Development

B.D. Gregory, R.C. O'Malley, Ryan Lister, M.A. Urich, Julian Tonti-Filippini, H. Chen, Harvey Millar, J.R. Ecker

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Abstract

MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are abundant endogenous small RNAs (smRNAs) that control transcript expression through posttranscriptional gene silencing. Here, we show that concomitant loss of XRN4/EIN5, a 5′-3′ exoribonuclease, and ABH1/CBP80, a subunit of the mRNA cap binding complex, results in Arabidopsis plants manifesting myriad developmental defects. We find that ABH1/CBP80 is necessary to obtain proper mature miRNA levels, which suggests this protein affects the miRNA-mediated RNA silencing pathway. Additionally, we show that XRN4/EIN5 affects the levels of a smRNA class that is processed from both sense and antisense strands of not, vert, similar130 endogenous transcripts that apparently are converted to double-stranded RNA (dsRNA) and subsequently processed. We find that the parent transcripts of these smRNAs accumulate in an uncapped form upon loss of XRN4/EIN5, which suggests that uncapped endogenous transcripts can become smRNA biogenesis substrates. Overall, our results reveal unexpected connections between RNA metabolism and silencing pathways.
Original languageEnglish
Pages (from-to)854-866
JournalDevelopmental Cell
Volume14
Issue number6
DOIs
Publication statusPublished - 2008

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    Gregory, B. D., O'Malley, R. C., Lister, R., Urich, M. A., Tonti-Filippini, J., Chen, H., Millar, H., & Ecker, J. R. (2008). A Link between RNA Metabolism and Silencing Affecting Arabidopsis Development. Developmental Cell, 14(6), 854-866. https://doi.org/10.1016/j.devcel.2008.04.005