A Link between RNA Metabolism and Silencing Affecting Arabidopsis Development

B.D. Gregory, R.C. O'Malley, Ryan Lister, M.A. Urich, Julian Tonti-Filippini, H. Chen, Harvey Millar, J.R. Ecker

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283 Citations (Scopus)
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MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are abundant endogenous small RNAs (smRNAs) that control transcript expression through posttranscriptional gene silencing. Here, we show that concomitant loss of XRN4/EIN5, a 5′-3′ exoribonuclease, and ABH1/CBP80, a subunit of the mRNA cap binding complex, results in Arabidopsis plants manifesting myriad developmental defects. We find that ABH1/CBP80 is necessary to obtain proper mature miRNA levels, which suggests this protein affects the miRNA-mediated RNA silencing pathway. Additionally, we show that XRN4/EIN5 affects the levels of a smRNA class that is processed from both sense and antisense strands of not, vert, similar130 endogenous transcripts that apparently are converted to double-stranded RNA (dsRNA) and subsequently processed. We find that the parent transcripts of these smRNAs accumulate in an uncapped form upon loss of XRN4/EIN5, which suggests that uncapped endogenous transcripts can become smRNA biogenesis substrates. Overall, our results reveal unexpected connections between RNA metabolism and silencing pathways.
Original languageEnglish
Pages (from-to)854-866
JournalDevelopmental Cell
Issue number6
Publication statusPublished - 2008


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