@article{58e73f5272524b528a1d395e9a06e66f,
title = "A library of induced pluripotent stem cells from clinically well-characterized, diverse healthy human individuals",
abstract = "A library of well-characterized human induced pluripotent stem cell (hiPSC) lines from clinically healthy human subjects could serve as a useful resource of normal controls for in vitro human development, disease modeling, genotype-phenotype association studies, and drug response evaluation. We report generation and extensive characterization of a gender-balanced, racially/ethnically diverse library of hiPSC lines from 40 clinically healthy human individuals who range in age from 22 to 61 years. The hiPSCs match the karyotype and short tandem repeat identities of their parental fibroblasts, and have a transcription profile characteristic of pluripotent stem cells. We provide whole-genome sequencing data for one hiPSC clone from each individual, genomic ancestry determination, and analysis of mendelian disease genes and risks. We document similar transcriptomic profiles, single-cell RNA-sequencing-derived cell clusters, and physiology of cardiomyocytes differentiated from multiple independent hiPSC lines. This extensive characterization makes this hiPSC library a valuable resource for many studies on human biology.",
keywords = "age- and sex-independent gene signature, cardiomyocytes, clinically well-characterized healthy subjects, genetic variants of mild common disease phenotypes or more severe disease with incomplete penetrance, induced pluripotent stem cells, racially/ethnically diverse, similar physiology and transcriptomes, whole-genome sequencing",
author = "Christoph Schaniel and Priyanka Dhanan and Bin Hu and Yuguang Xiong and Teeya Raghunandan and Gonzalez, {David M.} and Rafael Dariolli and D'Souza, {Sunita L.} and Yadaw, {Arjun S.} and Jens Hansen and Gomathi Jayaraman and Bino Mathew and Moara Machado and Berger, {Seth I.} and Joseph Tripodi and Vesna Najfeld and Jalaj Garg and Marc Miller and Surlyn, {Colleen S.} and Michelis, {Katherine C.} and Tangirala, {Neelima C.} and Himali Weerahandi and Thomas, {David C.} and Beaumont, {Kristin G.} and Robert Sebra and Milind Mahajan and Eric Schadt and Dusica Vidovic and Sch{\"u}rer, {Stephan C.} and Joseph Goldfarb and Azeloglu, {Evren U.} and Birtwistle, {Marc R.} and Sobie, {Eric A.} and Kovacic, {Jason C.} and Dubois, {Nicole C.} and Ravi Iyengar",
note = "Funding Information: This work was supported by NIH Common Fund LINCS program awards U54HG008098 (R.I.) and U54HL127624 (S.C.S). B.M., M.M., J.T., M.M., and E.S. received salaries from Sema4. We thank the Mount Sinai Clinical Research Unit for providing examination rooms and nurses, and the Mount Sinai Genomics Core for RNA-seq. Funding Information: This work was supported by NIH Common Fund LINCS program awards U54HG008098 (R.I.) and U54HL127624 (S.C.S). B.M. M.M. J.T. M.M. and E.S. received salaries from Sema4. We thank the Mount Sinai Clinical Research Unit for providing examination rooms and nurses, and the Mount Sinai Genomics Core for RNA-seq. Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2021",
month = dec,
day = "14",
doi = "10.1016/j.stemcr.2021.10.005",
language = "English",
volume = "16",
pages = "3036--3049",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "12",
}