TY - JOUR
T1 - A Key Role for Pax7 Transcripts in Determination of Muscle and Nerve Cells
AU - Ziman, Melanie
AU - Thomas, Meghan
AU - Jacobsen, P.
AU - Beazley, Lyn
PY - 2001
Y1 - 2001
N2 - Embryonic teratocarcinoma mouse cells (P19) and embryonic NIH3T3 fibroblasts were induced chemically to differentiate along neurogenic or myogenic lineages. The expression profiles of Pax7 alternate transcripts were then assessed by RNA isolation and RT-PCR. Only two transcripts, Pax7b and Pax7d, were expressed in the neurogenic lineage. By contrast, in adult skeletal muscle, four transcripts, Pax7a-d, were expressed in the myogenic lineage. Moreover, P19 cells were shown to undergo neural cell differentiation when stably transfected with a single Pax7 transcript, PAX7b, generated from human skeletal muscle. Our results suggest a key role for Pax7 transcripts in lineage determination. (C) 2001 Academic Press.
AB - Embryonic teratocarcinoma mouse cells (P19) and embryonic NIH3T3 fibroblasts were induced chemically to differentiate along neurogenic or myogenic lineages. The expression profiles of Pax7 alternate transcripts were then assessed by RNA isolation and RT-PCR. Only two transcripts, Pax7b and Pax7d, were expressed in the neurogenic lineage. By contrast, in adult skeletal muscle, four transcripts, Pax7a-d, were expressed in the myogenic lineage. Moreover, P19 cells were shown to undergo neural cell differentiation when stably transfected with a single Pax7 transcript, PAX7b, generated from human skeletal muscle. Our results suggest a key role for Pax7 transcripts in lineage determination. (C) 2001 Academic Press.
U2 - 10.1006/excr.2001.5282
DO - 10.1006/excr.2001.5282
M3 - Article
C2 - 11478848
SN - 0014-4827
VL - 268
SP - 220
EP - 229
JO - Experimental Cell Research
JF - Experimental Cell Research
ER -