A high-throughput MS-PCR method on MADGE gels for ANG II type-1 receptor A1166C polymorphism

Clive C.J. Hunt; Jodi E. Burley; Caroline M.L. Chapman; John P. Beilby

doi:10.1152/physiolgenomics.1999.1.2.71

A high-throughput MS-PCR method on MADGE gels for ANG II type-1 receptor A1166C polymorphism

Clive C.J. Hunt, Jodi E. Burley, Caroline M.L. Chapman, John P. Beilby

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We have developed a highly accurate, low-cost, single-step, mutagenically separated polymerase chain reaction (MS-PCR) method for the determination of angiotensin II type-1 receptor (AT₁)A1166C gene polymorphism. The genotypes are determined using the microtiter array diagonal gel electrophoresis (MADGE) system. We have compared the MS-PCR method with allele-specific oligonucleotide hybridization and Dde I digestion techniques for determining the AT₁ A1166C genotype. The combination of MS-PCR and MADGE serves as a model for high-throughput single-nucleotide polymorphism genotyping in large population studies.

Original language	English
Pages (from-to)	71-73
Number of pages	3
Journal	Physiological Genomics
Volume	1999
Issue number	1
DOIs	https://doi.org/10.1152/physiolgenomics.1999.1.2.71
Publication status	Published - Dec 1999
Externally published	Yes

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10.1152/physiolgenomics.1999.1.2.71

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title = "A high-throughput MS-PCR method on MADGE gels for ANG II type-1 receptor A1166C polymorphism",

abstract = "We have developed a highly accurate, low-cost, single-step, mutagenically separated polymerase chain reaction (MS-PCR) method for the determination of angiotensin II type-1 receptor (AT1)A1166C gene polymorphism. The genotypes are determined using the microtiter array diagonal gel electrophoresis (MADGE) system. We have compared the MS-PCR method with allele-specific oligonucleotide hybridization and Dde I digestion techniques for determining the AT1 A1166C genotype. The combination of MS-PCR and MADGE serves as a model for high-throughput single-nucleotide polymorphism genotyping in large population studies.",

keywords = "Angiotensin II type-1 receptor, Genetics, Microtiter array diagonal gel electrophoresis, Mutagenically separated polymerase chain reaction, Polymorphism, Receptors",

author = "Hunt, {Clive C.J.} and Burley, {Jodi E.} and Chapman, {Caroline M.L.} and Beilby, {John P.}",

year = "1999",

month = dec,

doi = "10.1152/physiolgenomics.1999.1.2.71",

language = "English",

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T1 - A high-throughput MS-PCR method on MADGE gels for ANG II type-1 receptor A1166C polymorphism

AU - Hunt, Clive C.J.

AU - Burley, Jodi E.

AU - Chapman, Caroline M.L.

AU - Beilby, John P.

PY - 1999/12

Y1 - 1999/12

N2 - We have developed a highly accurate, low-cost, single-step, mutagenically separated polymerase chain reaction (MS-PCR) method for the determination of angiotensin II type-1 receptor (AT1)A1166C gene polymorphism. The genotypes are determined using the microtiter array diagonal gel electrophoresis (MADGE) system. We have compared the MS-PCR method with allele-specific oligonucleotide hybridization and Dde I digestion techniques for determining the AT1 A1166C genotype. The combination of MS-PCR and MADGE serves as a model for high-throughput single-nucleotide polymorphism genotyping in large population studies.

AB - We have developed a highly accurate, low-cost, single-step, mutagenically separated polymerase chain reaction (MS-PCR) method for the determination of angiotensin II type-1 receptor (AT1)A1166C gene polymorphism. The genotypes are determined using the microtiter array diagonal gel electrophoresis (MADGE) system. We have compared the MS-PCR method with allele-specific oligonucleotide hybridization and Dde I digestion techniques for determining the AT1 A1166C genotype. The combination of MS-PCR and MADGE serves as a model for high-throughput single-nucleotide polymorphism genotyping in large population studies.

KW - Angiotensin II type-1 receptor

KW - Genetics

KW - Microtiter array diagonal gel electrophoresis

KW - Mutagenically separated polymerase chain reaction

KW - Polymorphism

KW - Receptors

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DO - 10.1152/physiolgenomics.1999.1.2.71

M3 - Article

C2 - 11015563

AN - SCOPUS:0033620973

SN - 1531-2267

VL - 1999

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EP - 73

JO - Physiological Genomics

JF - Physiological Genomics

IS - 1

ER -

A high-throughput MS-PCR method on MADGE gels for ANG II type-1 receptor A1166C polymorphism

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