TY - JOUR
T1 - A dual role of dLsd1 in oogenesis
T2 - regulating developmental genes and repressing transposons
AU - Lepesant, Julie M.J.
AU - Iampietro, Carole
AU - Galeota, Eugenia
AU - Augé, Benoit
AU - Aguirrenbengoa, Marion
AU - Mercé, Clemèntine
AU - Chaubet, Camille
AU - Rocher, Vincent
AU - Haenlin, Marc
AU - Waltzer, Lucas
AU - Pelizzola, Mattia
AU - Di Stefano, Luisa
PY - 2021
Y1 - 2021
N2 - The histone demethylase LSD1 is a key chromatin regulator that is often deregulated in cancer. Its ortholog, dLsd1 plays a crucial role in Drosophila oogenesis; however, our knowledge of dLsd1 function is insufficient to explain its role in the ovary. Here, we have performed genome-wide analysis of dLsd1 binding in the ovary, and we document that dLsd1 is preferentially associated to the transcription start site of developmental genes. We uncovered an unanticipated interplay between dLsd1 and the GATA transcription factor Serpent and we report an unexpected role for Serpent in oogenesis. Besides, our transcriptomic data show that reducing dLsd1 levels results in ectopic transposable elements (TE) expression correlated with changes in H3K4me2 and H3K9me2 at TE loci. In addition, our results suggest that dLsd1 is required for Piwi dependent TE silencing. Hence, we propose that dLsd1 plays crucial roles in establishing specific gene expression programs and in repressing transposons during oogenesis.
AB - The histone demethylase LSD1 is a key chromatin regulator that is often deregulated in cancer. Its ortholog, dLsd1 plays a crucial role in Drosophila oogenesis; however, our knowledge of dLsd1 function is insufficient to explain its role in the ovary. Here, we have performed genome-wide analysis of dLsd1 binding in the ovary, and we document that dLsd1 is preferentially associated to the transcription start site of developmental genes. We uncovered an unanticipated interplay between dLsd1 and the GATA transcription factor Serpent and we report an unexpected role for Serpent in oogenesis. Besides, our transcriptomic data show that reducing dLsd1 levels results in ectopic transposable elements (TE) expression correlated with changes in H3K4me2 and H3K9me2 at TE loci. In addition, our results suggest that dLsd1 is required for Piwi dependent TE silencing. Hence, we propose that dLsd1 plays crucial roles in establishing specific gene expression programs and in repressing transposons during oogenesis.
UR - http://www.scopus.com/inward/record.url?scp=85082145696&partnerID=8YFLogxK
U2 - 10.1093/nar/gkz1142
DO - 10.1093/nar/gkz1142
M3 - Article
C2 - 31799607
AN - SCOPUS:85082145696
SN - 0305-1048
VL - 48
SP - 1206
EP - 1224
JO - Nucleic acids research
JF - Nucleic acids research
IS - 3
ER -
