A double EPSPS gene mutation endowing glyphosate resistance shows a remarkably high resistance cost

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A novel glyphosate resistance double point mutation (T102I/P106S, TIPS) in the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene has been recently identified for the first time only in the weed species Eleusine indica. Quantification of plant resistance cost associated with the TIPS and the often reported glyphosate resistance single P106S mutation was performed. A significant resistance cost (50% in seed number currency) associated with the homozygous TIPS but not the homozygous P106S EPSPS variant was identified in E. indica plants. The resistance cost associated with the TIPS mutation escalated to 85% in plants under resource competition with rice crops. The resistance cost was not detected in nonhomozygous TIPS plants denoting the recessive nature of the cost associated with the TIPS allele. An excess of 11-fold more shikimate and sixfold more quinate in the shikimate pathway was detected in TIPS plants in the absence of glyphosate treatment compared to wild type, whereas no changes in these compounds were observed in P106S plants when compared to wild type. TIPS plants show altered metabolite levels in several other metabolic pathways that may account for the expression of the observed resistance cost.

Original languageEnglish
Pages (from-to)3031-3042
Number of pages12
JournalPlant Cell and Environment
Volume40
Issue number12
DOIs
Publication statusPublished - 1 Dec 2017

Fingerprint

glyphosate
3-Phosphoshikimate 1-Carboxyvinyltransferase
mutation
Costs and Cost Analysis
Mutation
Genes
genes
Eleusine
Quinic Acid
Eleusine indica
shikimate pathway
point mutation
Metabolic Networks and Pathways
3-phosphoshikimate 1-carboxyvinyltransferase
Point Mutation
biochemical pathways

Cite this

@article{2afce7ec1ce6409cbe68324c3148ee14,
title = "A double EPSPS gene mutation endowing glyphosate resistance shows a remarkably high resistance cost",
abstract = "A novel glyphosate resistance double point mutation (T102I/P106S, TIPS) in the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene has been recently identified for the first time only in the weed species Eleusine indica. Quantification of plant resistance cost associated with the TIPS and the often reported glyphosate resistance single P106S mutation was performed. A significant resistance cost (50{\%} in seed number currency) associated with the homozygous TIPS but not the homozygous P106S EPSPS variant was identified in E. indica plants. The resistance cost associated with the TIPS mutation escalated to 85{\%} in plants under resource competition with rice crops. The resistance cost was not detected in nonhomozygous TIPS plants denoting the recessive nature of the cost associated with the TIPS allele. An excess of 11-fold more shikimate and sixfold more quinate in the shikimate pathway was detected in TIPS plants in the absence of glyphosate treatment compared to wild type, whereas no changes in these compounds were observed in P106S plants when compared to wild type. TIPS plants show altered metabolite levels in several other metabolic pathways that may account for the expression of the observed resistance cost.",
keywords = "aromatic amino acids, glyphosate resistance cost, Pro-106-Ser, TIPS",
author = "Heping Han and Vila-Aiub, {Martin M.} and Adam Jalaludin and Qin Yu and Powles, {Stephen B.}",
year = "2017",
month = "12",
day = "1",
doi = "10.1111/pce.13067",
language = "English",
volume = "40",
pages = "3031--3042",
journal = "Plant, Cell and Environment.",
issn = "0140-7791",
publisher = "John Wiley & Sons",
number = "12",

}

TY - JOUR

T1 - A double EPSPS gene mutation endowing glyphosate resistance shows a remarkably high resistance cost

AU - Han, Heping

AU - Vila-Aiub, Martin M.

AU - Jalaludin, Adam

AU - Yu, Qin

AU - Powles, Stephen B.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - A novel glyphosate resistance double point mutation (T102I/P106S, TIPS) in the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene has been recently identified for the first time only in the weed species Eleusine indica. Quantification of plant resistance cost associated with the TIPS and the often reported glyphosate resistance single P106S mutation was performed. A significant resistance cost (50% in seed number currency) associated with the homozygous TIPS but not the homozygous P106S EPSPS variant was identified in E. indica plants. The resistance cost associated with the TIPS mutation escalated to 85% in plants under resource competition with rice crops. The resistance cost was not detected in nonhomozygous TIPS plants denoting the recessive nature of the cost associated with the TIPS allele. An excess of 11-fold more shikimate and sixfold more quinate in the shikimate pathway was detected in TIPS plants in the absence of glyphosate treatment compared to wild type, whereas no changes in these compounds were observed in P106S plants when compared to wild type. TIPS plants show altered metabolite levels in several other metabolic pathways that may account for the expression of the observed resistance cost.

AB - A novel glyphosate resistance double point mutation (T102I/P106S, TIPS) in the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene has been recently identified for the first time only in the weed species Eleusine indica. Quantification of plant resistance cost associated with the TIPS and the often reported glyphosate resistance single P106S mutation was performed. A significant resistance cost (50% in seed number currency) associated with the homozygous TIPS but not the homozygous P106S EPSPS variant was identified in E. indica plants. The resistance cost associated with the TIPS mutation escalated to 85% in plants under resource competition with rice crops. The resistance cost was not detected in nonhomozygous TIPS plants denoting the recessive nature of the cost associated with the TIPS allele. An excess of 11-fold more shikimate and sixfold more quinate in the shikimate pathway was detected in TIPS plants in the absence of glyphosate treatment compared to wild type, whereas no changes in these compounds were observed in P106S plants when compared to wild type. TIPS plants show altered metabolite levels in several other metabolic pathways that may account for the expression of the observed resistance cost.

KW - aromatic amino acids

KW - glyphosate resistance cost

KW - Pro-106-Ser

KW - TIPS

UR - http://www.scopus.com/inward/record.url?scp=85034262917&partnerID=8YFLogxK

U2 - 10.1111/pce.13067

DO - 10.1111/pce.13067

M3 - Article

VL - 40

SP - 3031

EP - 3042

JO - Plant, Cell and Environment.

JF - Plant, Cell and Environment.

SN - 0140-7791

IS - 12

ER -