The bitterness of racemic praziquantel (Rac-PZQ) constrains its use as an in-feed treatment against monogenean flukes in finfish aquaculture. Evidence exists in mammals that the R-(−) enantiomer of PZQ is less bitter than the S-(+) enantiomer. If fish exhibit this same response, then the recently described techniques for the large-scale resolution of R-(−)-PZQ from Rac-PZQ could facilitate the wide-spread application of this effective anthelmintic compound via feed. The hypothesis that yellowtail kingfish Seriola lalandi would find R-(−)-PZQ more palatable than Rac-PZQ and S-(+)-PZQ was tested in four trials. During the first three trials, the palatability of diets top-coated with 10 g kg−1 of Rac-PZQ or its two enantioseparated isomers were compared in small (85–160 g) and large (1.2 kg) yellowtail kingfish. A fourth trial compared the palatability of R-(−)-PZQ and Rac-PZQ at dietary inclusion levels of 2.5, 5.0 and 10.0 g kg−1 in small yellowtail kingfish (170 g). Ingestion data showed that R-(−)-PZQ to be no more palatable than either Rac-PZQ or S-(+)-PZQ to yellowtail kingfish, regardless of size. Indeed, evidence suggested that the S-(+)-PZQ to be slightly more palatable than both R-(−)-PZQ and Rac-PZQ. From these data, we hypothesize that the strong smell of R-(−)-PZQ (which was not present in S-(+)-PZQ) is an equally important determinant to palatability as taste in yellowtail kingfish. Results demonstrate that dietary inclusion level is a more important determinant to palatability than PZQ chirality; however, administration of R-(−)-PZQ may still be advantageous if it is demonstrated to be the only enantiomer efficacious against monogeneans.