A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A

A. Cruz-Migoni; G.M. M. Hautbergue; P.J. J. Artymiuk; P.J. J. Baker; M. Bokori-Brown; C.T. T. Chang; M.J. J. Dickman; A. Essex-Lopresti; S.V. V. Harding; N.M. M. Mahadi; L.E. E. Marshall; G.W. W. Mobbs; R. Mohamed; S. Nathan; S.A. A. Ngugi; C. Ong; W.F. F. Ooi; L.J. J. Partridge; H.L. L. Phillips; M.F. F. Raih; S. Ruzheinikov; Mitali Sarkar-Tyson; S.E. E. Sedelnikova; S.J. J. Smither; P. Tan; R.W. W. Titball; S.A. A. Wilson; D.W. W. Rice

doi:10.1126/science.1211915

A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A

A. Cruz-Migoni, G.M. M. Hautbergue, P.J. J. Artymiuk, P.J. J. Baker, M. Bokori-Brown, C.T. T. Chang, M.J. J. Dickman, A. Essex-Lopresti, S.V. V. Harding, N.M. M. Mahadi, L.E. E. Marshall, G.W. W. Mobbs, R. Mohamed, S. Nathan, S.A. A. Ngugi, C. Ong, W.F. F. Ooi, L.J. J. Partridge, H.L. L. Phillips, M.F. F. RaihS. Ruzheinikov, Mitali Sarkar-Tyson, S.E. E. Sedelnikova, S.J. J. Smither, P. Tan, R.W. W. Titball, S.A. A. Wilson, D.W. W. Rice

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89 Citations (Scopus)

Abstract

The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.

Original language	English
Pages (from-to)	821-824
Number of pages	4
Journal	Science
Volume	334
Issue number	6057
DOIs	https://doi.org/10.1126/science.1211915
Publication status	Published - 2011
Externally published	Yes

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Cruz-Migoni, A., Hautbergue, G. M. M., Artymiuk, P. J. J., Baker, P. J. J., Bokori-Brown, M., Chang, C. T. T., Dickman, M. J. J., Essex-Lopresti, A., Harding, S. V. V., Mahadi, N. M. M., Marshall, L. E. E., Mobbs, G. W. W., Mohamed, R., Nathan, S., Ngugi, S. A. A., Ong, C., Ooi, W. F. F., Partridge, L. J. J., Phillips, H. L. L., ... Rice, D. W. W. (2011). A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A. Science, 334(6057), 821-824. https://doi.org/10.1126/science.1211915

@article{006d089ff48841599e4650d8bc53355e,

title = "A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A",

abstract = "The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.",

author = "A. Cruz-Migoni and Hautbergue, {G.M. M.} and Artymiuk, {P.J. J.} and Baker, {P.J. J.} and M. Bokori-Brown and Chang, {C.T. T.} and Dickman, {M.J. J.} and A. Essex-Lopresti and Harding, {S.V. V.} and Mahadi, {N.M. M.} and Marshall, {L.E. E.} and Mobbs, {G.W. W.} and R. Mohamed and S. Nathan and Ngugi, {S.A. A.} and C. Ong and Ooi, {W.F. F.} and Partridge, {L.J. J.} and Phillips, {H.L. L.} and Raih, {M.F. F.} and S. Ruzheinikov and Mitali Sarkar-Tyson and Sedelnikova, {S.E. E.} and Smither, {S.J. J.} and P. Tan and Titball, {R.W. W.} and Wilson, {S.A. A.} and Rice, {D.W. W.}",

year = "2011",

doi = "10.1126/science.1211915",

language = "English",

volume = "334",

pages = "821--824",

journal = "Science",

issn = "0036-8075",

publisher = "AMER ASSOC ADVANCEMENT SCIENCE",

number = "6057",

}

Cruz-Migoni, A, Hautbergue, GMM, Artymiuk, PJJ, Baker, PJJ, Bokori-Brown, M, Chang, CTT, Dickman, MJJ, Essex-Lopresti, A, Harding, SVV, Mahadi, NMM, Marshall, LEE, Mobbs, GWW, Mohamed, R, Nathan, S, Ngugi, SAA, Ong, C, Ooi, WFF, Partridge, LJJ, Phillips, HLL, Raih, MFF, Ruzheinikov, S, Sarkar-Tyson, M, Sedelnikova, SEE, Smither, SJJ, Tan, P, Titball, RWW, Wilson, SAA & Rice, DWW 2011, 'A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A', Science, vol. 334, no. 6057, pp. 821-824. https://doi.org/10.1126/science.1211915

TY - JOUR

T1 - A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A

AU - Cruz-Migoni, A.

AU - Hautbergue, G.M. M.

AU - Artymiuk, P.J. J.

AU - Baker, P.J. J.

AU - Bokori-Brown, M.

AU - Chang, C.T. T.

AU - Dickman, M.J. J.

AU - Essex-Lopresti, A.

AU - Harding, S.V. V.

AU - Mahadi, N.M. M.

AU - Marshall, L.E. E.

AU - Mobbs, G.W. W.

AU - Mohamed, R.

AU - Nathan, S.

AU - Ngugi, S.A. A.

AU - Ong, C.

AU - Ooi, W.F. F.

AU - Partridge, L.J. J.

AU - Phillips, H.L. L.

AU - Raih, M.F. F.

AU - Ruzheinikov, S.

AU - Sarkar-Tyson, Mitali

AU - Sedelnikova, S.E. E.

AU - Smither, S.J. J.

AU - Tan, P.

AU - Titball, R.W. W.

AU - Wilson, S.A. A.

AU - Rice, D.W. W.

PY - 2011

Y1 - 2011

N2 - The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.

AB - The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.

U2 - 10.1126/science.1211915

DO - 10.1126/science.1211915

M3 - Article

SN - 0036-8075

VL - 334

SP - 821

EP - 824

JO - Science

JF - Science

IS - 6057

ER -

A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A

Abstract

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