A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A

A. Cruz-Migoni, G.M. M. Hautbergue, P.J. J. Artymiuk, P.J. J. Baker, M. Bokori-Brown, C.T. T. Chang, M.J. J. Dickman, A. Essex-Lopresti, S.V. V. Harding, N.M. M. Mahadi, L.E. E. Marshall, G.W. W. Mobbs, R. Mohamed, S. Nathan, S.A. A. Ngugi, C. Ong, W.F. F. Ooi, L.J. J. Partridge, H.L. L. Phillips, M.F. F. RaihS. Ruzheinikov, Mitali Sarkar-Tyson, S.E. E. Sedelnikova, S.J. J. Smither, P. Tan, R.W. W. Titball, S.A. A. Wilson, D.W. W. Rice

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.
Original languageEnglish
Pages (from-to)821-824
Number of pages4
JournalScience
Volume334
Issue number6057
DOIs
Publication statusPublished - 2011
Externally publishedYes

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