A bioassay for prosulfocarb, pyroxasulfone and trifluralin detection and quantification in soil and crop residues

Yaseen Khalil, Kadambot H.M. Siddique, Phil Ward, Colin Piggin, Sze How Bong, Shabarinath Nambiar, Robert Trengove, Ken Flower

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Three experiments were conducted to develop a bioassay method for assessing the bioavailability of prosulfocarb, pyroxasulfone and trifluralin in both crop residue and soil. In preliminary experiments, Italian ryegrass (Lolium multiflorum Lam.), cucumber (Cucumis sativus L.) and beetroot (Beta vulgaris L.) were tested as bioassay plant species for the three pre-emergent herbicides. Four growth parameters (shoot length, root length, fresh weight and dry weight) were measured for all plant species. Shoot-length inhibition was identified as the most responsive to the herbicide application rates. Italian ryegrass was the most sensitive species to all tested herbicides, whereas beetroot and cucumber had lower and similar sensitivity to shoot inhibition for the three herbicides. The bioassay species performed similarly in wheat and canola residues collected a few days after harvest. In bioassay calibration experiments, dose-response curves were developed for prosulfocarb, pyroxasulfone and trifluralin in a sandy loam soil typical of the grain belt of Western Australia and with wheat residue. The developed bioassay uses ryegrass shoot inhibition for relatively low suspected concentrations of herbicide, and cucumber shoot inhibition for higher rates. The bioassay was validated by spraying the three herbicides separately onto wheat residue and soil and comparing the concentrations derived from chemical analysis with those from the bioassay. All of the linear correlations between concentrations derived from chemical analyses and the bioassays were highly significant. These results indicate that the bioassay calibration curves are suitable for estimating herbicide concentrations in crop residue collected soon after harvest and a sandy-loam soil, low in organic matter.

Original languageEnglish
Pages (from-to)606-616
Number of pages11
JournalCrop & Pasture Science
Volume69
Issue number6
DOIs
Publication statusPublished - 2018

Fingerprint

pyroxasulfone
trifluralin
crop residues
bioassays
herbicides
soil
Lolium multiflorum
shoots
cucumbers
beets
sandy loam soils
wheat
calibration
Beta vulgaris
Cucumis sativus
canola
Lolium
chemical analysis
Western Australia
spraying

Cite this

Khalil, Yaseen ; Siddique, Kadambot H.M. ; Ward, Phil ; Piggin, Colin ; Bong, Sze How ; Nambiar, Shabarinath ; Trengove, Robert ; Flower, Ken. / A bioassay for prosulfocarb, pyroxasulfone and trifluralin detection and quantification in soil and crop residues. In: Crop & Pasture Science. 2018 ; Vol. 69, No. 6. pp. 606-616.
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A bioassay for prosulfocarb, pyroxasulfone and trifluralin detection and quantification in soil and crop residues. / Khalil, Yaseen; Siddique, Kadambot H.M.; Ward, Phil; Piggin, Colin; Bong, Sze How; Nambiar, Shabarinath; Trengove, Robert; Flower, Ken.

In: Crop & Pasture Science, Vol. 69, No. 6, 2018, p. 606-616.

Research output: Contribution to journalArticle

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T1 - A bioassay for prosulfocarb, pyroxasulfone and trifluralin detection and quantification in soil and crop residues

AU - Khalil, Yaseen

AU - Siddique, Kadambot H.M.

AU - Ward, Phil

AU - Piggin, Colin

AU - Bong, Sze How

AU - Nambiar, Shabarinath

AU - Trengove, Robert

AU - Flower, Ken

PY - 2018

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N2 - Three experiments were conducted to develop a bioassay method for assessing the bioavailability of prosulfocarb, pyroxasulfone and trifluralin in both crop residue and soil. In preliminary experiments, Italian ryegrass (Lolium multiflorum Lam.), cucumber (Cucumis sativus L.) and beetroot (Beta vulgaris L.) were tested as bioassay plant species for the three pre-emergent herbicides. Four growth parameters (shoot length, root length, fresh weight and dry weight) were measured for all plant species. Shoot-length inhibition was identified as the most responsive to the herbicide application rates. Italian ryegrass was the most sensitive species to all tested herbicides, whereas beetroot and cucumber had lower and similar sensitivity to shoot inhibition for the three herbicides. The bioassay species performed similarly in wheat and canola residues collected a few days after harvest. In bioassay calibration experiments, dose-response curves were developed for prosulfocarb, pyroxasulfone and trifluralin in a sandy loam soil typical of the grain belt of Western Australia and with wheat residue. The developed bioassay uses ryegrass shoot inhibition for relatively low suspected concentrations of herbicide, and cucumber shoot inhibition for higher rates. The bioassay was validated by spraying the three herbicides separately onto wheat residue and soil and comparing the concentrations derived from chemical analysis with those from the bioassay. All of the linear correlations between concentrations derived from chemical analyses and the bioassays were highly significant. These results indicate that the bioassay calibration curves are suitable for estimating herbicide concentrations in crop residue collected soon after harvest and a sandy-loam soil, low in organic matter.

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KW - root length inhibition

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SN - 1836-0947

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