TY - JOUR
T1 - A 3D map of the human genome at kilobase resolution reveals principles of chromatin looping
AU - Rao, Suhas S.P.
AU - Huntley, Miriam H.
AU - Durand, Neva C.
AU - Stamenova, Elena K.
AU - Bochkov, Ivan D.
AU - Robinson, James T.
AU - Sanborn, Adrian L.
AU - Machol, Ido
AU - Omer, Arina D.
AU - Lander, Eric S.
AU - Aiden, Erez Lieberman
PY - 2014/12/18
Y1 - 2014/12/18
N2 - We use in situ Hi-C to probe the 3D architecture of genomes, constructing haploid and diploid maps of nine cell types. The densest, in human lymphoblastoid cells, contains 4.9 billion contacts, achieving 1 kb resolution. We find that genomes are partitioned into contact domains (median length, 185 kb), which are associated with distinct patterns of histone marks and segregate into six subcompartments. We identify ∼10,000 loops. These loops frequently link promoters and enhancers, correlate with gene activation, and show conservation across cell types and species. Loop anchors typically occur at domain boundaries and bind CTCF. CTCF sites at loop anchors occur predominantly (>90%) in a convergent orientation, with the asymmetric motifs "facing" one another. The inactive X chromosome splits into two massive domains and contains large loops anchored at CTCF-binding repeats. PaperFlick
AB - We use in situ Hi-C to probe the 3D architecture of genomes, constructing haploid and diploid maps of nine cell types. The densest, in human lymphoblastoid cells, contains 4.9 billion contacts, achieving 1 kb resolution. We find that genomes are partitioned into contact domains (median length, 185 kb), which are associated with distinct patterns of histone marks and segregate into six subcompartments. We identify ∼10,000 loops. These loops frequently link promoters and enhancers, correlate with gene activation, and show conservation across cell types and species. Loop anchors typically occur at domain boundaries and bind CTCF. CTCF sites at loop anchors occur predominantly (>90%) in a convergent orientation, with the asymmetric motifs "facing" one another. The inactive X chromosome splits into two massive domains and contains large loops anchored at CTCF-binding repeats. PaperFlick
UR - https://www.scopus.com/pages/publications/84919949716
U2 - 10.1016/j.cell.2014.11.021
DO - 10.1016/j.cell.2014.11.021
M3 - Article
C2 - 25497547
AN - SCOPUS:84919949716
SN - 0092-8674
VL - 159
SP - 1665
EP - 1680
JO - Cell
JF - Cell
IS - 7
ER -
