5-aminolevolinic acid enhances sunflower resistance to Orobanche cumana (Broomrape)

Juanjuan Li, Chong Yang, Hui Liu, Mengting Cao, Guijun Yan, Ping Si, Weijun Zhou, Ling Xu

Research output: Contribution to journalArticle

Abstract

The root holoparasitic weed sunflower broomrape (Orobanche cumana Wallr.) specifically infects sunflowers (Helianthus annuus L.) and causes significant loss of their quality and production. This experiment was designed to evaluate the ameliorative effects of 5-aminolevolinic acid (ALA) on susceptible sunflower (cv. TK0409) under O. cumana stress. Sunflower seeds were pretreated at five concentrations (0, 5, 10, 15, or 20 mg L−1) of ALA, and then the germinated seeds were grown in O. cumana infested and non-infested soil. Results revealed that malondialdehyde (MDA) and reactive oxygen species (ROS) were significantly increased in both roots and leaves along with O. cumana penetration, suggesting oxidative bursts in sunflower, whereas exogenously applied ALA significantly reduced the levels of MDA and ROS by inducing anti- and non-antioxidant enzyme activities under O. cumana stress. Besides, ALA obviously recovered the ultrastructural damages of broomrape-invaded leaf mesophyll and root tip cells in sunflower. Moreover, application of ALA significantly decreased the expressions of xyloglucan endotransglucosylase/hydrolase related genes (XTH6 and XTH9) in sunflower under O. cumana stress compared with the O. cumana infected plants alone, indicating that the increased resistance of sunflower might be related to hypersensitive reactions which were activated by ALA. All the applied dosages of ALA had the effects of enhancing the resistance of sunflower against O. cumana, and the most effective mitigation was observed at 10 mg L−1 ALA pretreatment. In conclusion, ALA enhanced sunflower resistance to broomrape by promoting antioxidant defence system, reducing ROS levels, decreasing cellular damage and regulating the expressions of stress related genes.

Original languageEnglish
Article number111467
JournalIndustrial Crops and Products
Volume140
DOIs
Publication statusPublished - 15 Nov 2019

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Orobanche cernua
Helianthus annuus
acids
reactive oxygen species
malondialdehyde
xyloglucan:xyloglucosyl transferase
sunflower seed
hypersensitive response
hydrolases
root tips
mesophyll
leaves
genes
pretreatment
antioxidant activity
weeds
enzyme activity

Cite this

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title = "5-aminolevolinic acid enhances sunflower resistance to Orobanche cumana (Broomrape)",
abstract = "The root holoparasitic weed sunflower broomrape (Orobanche cumana Wallr.) specifically infects sunflowers (Helianthus annuus L.) and causes significant loss of their quality and production. This experiment was designed to evaluate the ameliorative effects of 5-aminolevolinic acid (ALA) on susceptible sunflower (cv. TK0409) under O. cumana stress. Sunflower seeds were pretreated at five concentrations (0, 5, 10, 15, or 20 mg L−1) of ALA, and then the germinated seeds were grown in O. cumana infested and non-infested soil. Results revealed that malondialdehyde (MDA) and reactive oxygen species (ROS) were significantly increased in both roots and leaves along with O. cumana penetration, suggesting oxidative bursts in sunflower, whereas exogenously applied ALA significantly reduced the levels of MDA and ROS by inducing anti- and non-antioxidant enzyme activities under O. cumana stress. Besides, ALA obviously recovered the ultrastructural damages of broomrape-invaded leaf mesophyll and root tip cells in sunflower. Moreover, application of ALA significantly decreased the expressions of xyloglucan endotransglucosylase/hydrolase related genes (XTH6 and XTH9) in sunflower under O. cumana stress compared with the O. cumana infected plants alone, indicating that the increased resistance of sunflower might be related to hypersensitive reactions which were activated by ALA. All the applied dosages of ALA had the effects of enhancing the resistance of sunflower against O. cumana, and the most effective mitigation was observed at 10 mg L−1 ALA pretreatment. In conclusion, ALA enhanced sunflower resistance to broomrape by promoting antioxidant defence system, reducing ROS levels, decreasing cellular damage and regulating the expressions of stress related genes.",
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5-aminolevolinic acid enhances sunflower resistance to Orobanche cumana (Broomrape). / Li, Juanjuan; Yang, Chong; Liu, Hui; Cao, Mengting; Yan, Guijun; Si, Ping; Zhou, Weijun; Xu, Ling.

In: Industrial Crops and Products, Vol. 140, 111467, 15.11.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - 5-aminolevolinic acid enhances sunflower resistance to Orobanche cumana (Broomrape)

AU - Li, Juanjuan

AU - Yang, Chong

AU - Liu, Hui

AU - Cao, Mengting

AU - Yan, Guijun

AU - Si, Ping

AU - Zhou, Weijun

AU - Xu, Ling

PY - 2019/11/15

Y1 - 2019/11/15

N2 - The root holoparasitic weed sunflower broomrape (Orobanche cumana Wallr.) specifically infects sunflowers (Helianthus annuus L.) and causes significant loss of their quality and production. This experiment was designed to evaluate the ameliorative effects of 5-aminolevolinic acid (ALA) on susceptible sunflower (cv. TK0409) under O. cumana stress. Sunflower seeds were pretreated at five concentrations (0, 5, 10, 15, or 20 mg L−1) of ALA, and then the germinated seeds were grown in O. cumana infested and non-infested soil. Results revealed that malondialdehyde (MDA) and reactive oxygen species (ROS) were significantly increased in both roots and leaves along with O. cumana penetration, suggesting oxidative bursts in sunflower, whereas exogenously applied ALA significantly reduced the levels of MDA and ROS by inducing anti- and non-antioxidant enzyme activities under O. cumana stress. Besides, ALA obviously recovered the ultrastructural damages of broomrape-invaded leaf mesophyll and root tip cells in sunflower. Moreover, application of ALA significantly decreased the expressions of xyloglucan endotransglucosylase/hydrolase related genes (XTH6 and XTH9) in sunflower under O. cumana stress compared with the O. cumana infected plants alone, indicating that the increased resistance of sunflower might be related to hypersensitive reactions which were activated by ALA. All the applied dosages of ALA had the effects of enhancing the resistance of sunflower against O. cumana, and the most effective mitigation was observed at 10 mg L−1 ALA pretreatment. In conclusion, ALA enhanced sunflower resistance to broomrape by promoting antioxidant defence system, reducing ROS levels, decreasing cellular damage and regulating the expressions of stress related genes.

AB - The root holoparasitic weed sunflower broomrape (Orobanche cumana Wallr.) specifically infects sunflowers (Helianthus annuus L.) and causes significant loss of their quality and production. This experiment was designed to evaluate the ameliorative effects of 5-aminolevolinic acid (ALA) on susceptible sunflower (cv. TK0409) under O. cumana stress. Sunflower seeds were pretreated at five concentrations (0, 5, 10, 15, or 20 mg L−1) of ALA, and then the germinated seeds were grown in O. cumana infested and non-infested soil. Results revealed that malondialdehyde (MDA) and reactive oxygen species (ROS) were significantly increased in both roots and leaves along with O. cumana penetration, suggesting oxidative bursts in sunflower, whereas exogenously applied ALA significantly reduced the levels of MDA and ROS by inducing anti- and non-antioxidant enzyme activities under O. cumana stress. Besides, ALA obviously recovered the ultrastructural damages of broomrape-invaded leaf mesophyll and root tip cells in sunflower. Moreover, application of ALA significantly decreased the expressions of xyloglucan endotransglucosylase/hydrolase related genes (XTH6 and XTH9) in sunflower under O. cumana stress compared with the O. cumana infected plants alone, indicating that the increased resistance of sunflower might be related to hypersensitive reactions which were activated by ALA. All the applied dosages of ALA had the effects of enhancing the resistance of sunflower against O. cumana, and the most effective mitigation was observed at 10 mg L−1 ALA pretreatment. In conclusion, ALA enhanced sunflower resistance to broomrape by promoting antioxidant defence system, reducing ROS levels, decreasing cellular damage and regulating the expressions of stress related genes.

KW - 5-aminolevolinic acid

KW - Orobanche cumana

KW - Oxidative burst

KW - Ultrastructural damages

KW - Xyloglucan endotransglucosylase/hydrolase

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DO - 10.1016/j.indcrop.2019.111467

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JO - Industrial Crops and Products

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SN - 0926-6690

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