28-homobrassinolide regulates antioxidant enzyme activities and gene expression in response to salt- and temperature-induced oxidative stress in Brassica juncea

Harpreet Kaur, Geetika Sirhindi, Renu Bhardwaj, M. N. Alyemeni, Kadambot H. M. Siddique, Parvaiz Ahmad

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Brassinosteroids (BRs) are a group of naturally occurring plant steroid hormones that can induce plant tolerance to various plant stresses by regulating ROS production in cells, but the underlying mechanisms of this scavenging activity by BRs are not well understood. This study investigated the effects of 28-homobrassinolide (28-HBL) seed priming on Brassica juncea seedlings subjected to the combined stress of extreme temperatures (low, 4 degrees C or high, 44 degrees C) and salinity (180 mM), either alone or supplemented with 28-HBL treatments (0, 10(-6), 10(-9), 10(-12) M). The combined temperature and salt stress treatments significantly reduced shoot and root lengths, but these improved when supplemented with 28-HBL although the response was dose-dependent. The combined stress alone significantly increased H2O2 content, but was inhibited when supplemented with 28-HBL. The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APOX), glutathione reductase (GR), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) increased in response to 28-HBL. Overall, the 28-HBL seed priming treatment improved the plant's potential to combat the toxic effects imposed by the combined temperature and salt stress by tightly regulating the accumulation of ROS, which was reflected in the improved redox state of antioxidants.

Original languageEnglish
Article number8735
Number of pages13
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 2018

Cite this

@article{0a91b85e7dff4b2bafb64efbb77af812,
title = "28-homobrassinolide regulates antioxidant enzyme activities and gene expression in response to salt- and temperature-induced oxidative stress in Brassica juncea",
abstract = "Brassinosteroids (BRs) are a group of naturally occurring plant steroid hormones that can induce plant tolerance to various plant stresses by regulating ROS production in cells, but the underlying mechanisms of this scavenging activity by BRs are not well understood. This study investigated the effects of 28-homobrassinolide (28-HBL) seed priming on Brassica juncea seedlings subjected to the combined stress of extreme temperatures (low, 4 degrees C or high, 44 degrees C) and salinity (180 mM), either alone or supplemented with 28-HBL treatments (0, 10(-6), 10(-9), 10(-12) M). The combined temperature and salt stress treatments significantly reduced shoot and root lengths, but these improved when supplemented with 28-HBL although the response was dose-dependent. The combined stress alone significantly increased H2O2 content, but was inhibited when supplemented with 28-HBL. The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APOX), glutathione reductase (GR), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) increased in response to 28-HBL. Overall, the 28-HBL seed priming treatment improved the plant's potential to combat the toxic effects imposed by the combined temperature and salt stress by tightly regulating the accumulation of ROS, which was reflected in the improved redox state of antioxidants.",
keywords = "BEAN PHASEOLUS-VULGARIS, GLYCINE-MAX L., REAL-TIME PCR, HYDROGEN-PEROXIDE, MONODEHYDROASCORBATE REDUCTASE, GLUTATHIONE-REDUCTASE, SPINACH-CHLOROPLASTS, LIPID-PEROXIDATION, SALINITY STRESS, HEAT-STRESS",
author = "Harpreet Kaur and Geetika Sirhindi and Renu Bhardwaj and Alyemeni, {M. N.} and Siddique, {Kadambot H. M.} and Parvaiz Ahmad",
year = "2018",
month = "12",
doi = "10.1038/s41598-018-27032-w",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group - Macmillan Publishers",
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28-homobrassinolide regulates antioxidant enzyme activities and gene expression in response to salt- and temperature-induced oxidative stress in Brassica juncea. / Kaur, Harpreet; Sirhindi, Geetika; Bhardwaj, Renu; Alyemeni, M. N.; Siddique, Kadambot H. M.; Ahmad, Parvaiz.

In: Scientific Reports, Vol. 8, No. 1, 8735, 12.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - 28-homobrassinolide regulates antioxidant enzyme activities and gene expression in response to salt- and temperature-induced oxidative stress in Brassica juncea

AU - Kaur, Harpreet

AU - Sirhindi, Geetika

AU - Bhardwaj, Renu

AU - Alyemeni, M. N.

AU - Siddique, Kadambot H. M.

AU - Ahmad, Parvaiz

PY - 2018/12

Y1 - 2018/12

N2 - Brassinosteroids (BRs) are a group of naturally occurring plant steroid hormones that can induce plant tolerance to various plant stresses by regulating ROS production in cells, but the underlying mechanisms of this scavenging activity by BRs are not well understood. This study investigated the effects of 28-homobrassinolide (28-HBL) seed priming on Brassica juncea seedlings subjected to the combined stress of extreme temperatures (low, 4 degrees C or high, 44 degrees C) and salinity (180 mM), either alone or supplemented with 28-HBL treatments (0, 10(-6), 10(-9), 10(-12) M). The combined temperature and salt stress treatments significantly reduced shoot and root lengths, but these improved when supplemented with 28-HBL although the response was dose-dependent. The combined stress alone significantly increased H2O2 content, but was inhibited when supplemented with 28-HBL. The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APOX), glutathione reductase (GR), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) increased in response to 28-HBL. Overall, the 28-HBL seed priming treatment improved the plant's potential to combat the toxic effects imposed by the combined temperature and salt stress by tightly regulating the accumulation of ROS, which was reflected in the improved redox state of antioxidants.

AB - Brassinosteroids (BRs) are a group of naturally occurring plant steroid hormones that can induce plant tolerance to various plant stresses by regulating ROS production in cells, but the underlying mechanisms of this scavenging activity by BRs are not well understood. This study investigated the effects of 28-homobrassinolide (28-HBL) seed priming on Brassica juncea seedlings subjected to the combined stress of extreme temperatures (low, 4 degrees C or high, 44 degrees C) and salinity (180 mM), either alone or supplemented with 28-HBL treatments (0, 10(-6), 10(-9), 10(-12) M). The combined temperature and salt stress treatments significantly reduced shoot and root lengths, but these improved when supplemented with 28-HBL although the response was dose-dependent. The combined stress alone significantly increased H2O2 content, but was inhibited when supplemented with 28-HBL. The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APOX), glutathione reductase (GR), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR) increased in response to 28-HBL. Overall, the 28-HBL seed priming treatment improved the plant's potential to combat the toxic effects imposed by the combined temperature and salt stress by tightly regulating the accumulation of ROS, which was reflected in the improved redox state of antioxidants.

KW - BEAN PHASEOLUS-VULGARIS

KW - GLYCINE-MAX L.

KW - REAL-TIME PCR

KW - HYDROGEN-PEROXIDE

KW - MONODEHYDROASCORBATE REDUCTASE

KW - GLUTATHIONE-REDUCTASE

KW - SPINACH-CHLOROPLASTS

KW - LIPID-PEROXIDATION

KW - SALINITY STRESS

KW - HEAT-STRESS

U2 - 10.1038/s41598-018-27032-w

DO - 10.1038/s41598-018-27032-w

M3 - Article

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 8735

ER -