In order to examine the merits of buckwheat, four main flavonoids (rutin, kaempferol-3-rutinoside, quercetin and kaempferol) were analyzed by using reversed-phase HPLC in 21 kinds of buckwheat, with chromatography column of Damonsil C18 (4.6 mm × 250 mm, 5 μm), mobile phase of acetonitrile-0.2% acetic acid solution, flow rate of 1.0 mL/min, detection wavelength of 354 nm, and column temperature of 30°C. The results showed an average moisture content of 4.907 3-6.993 6% in 21 kinds of different species of buckwheat seeds at room temperature; under constant weight state, the bran mass fraction was 21.84-30.03%, accounting for 1/4 of buckwheat seeds quality. The linear ranges of rutin, kaempferol-3-rutinoside, quercetin and kaempferol were 20-400 μg/mL, 2-40 μg/mL, 1-20 μg/mL, 0.5-10 μg/mL, respectively. In the powder of 21 kinds of buckwheat seeds, the content ranges of rutin, kaempferol-3-rutinoside, quercetin and kaempferol were 12.137 0-20.318 6 mg/g, 1.478 7-2.871 9 mg/g, 0.014 6-0.901 5 mg/g, 0-0.015 9 mg/g, respectively. The total content of the four main flavonoids was 14.015 1-22.871 7 mg/g. In seeds bran, the content ranges of rutin, kaempferol-3-rutinoside, quercetin and kaempferol were 4.269 0-12.118 9 mg/g, 0.258 5-1.014 9 mg/g, 0.061 8-0.735 4 mg/g, 0-0.026 3 mg/g, respectively. The total content of the four main flavonoids was 4.651 1-13.148 6 mg/g. The results indicated that whether in seed powder or bran, rutin is the flavonoid of the highest contents, followed by kaempferol-3-rutinoside and quercetin, with kaempferol levels the lowest, less than one in ten thousandth. This study provides some important evidences for the use of certain kinds of buckwheat.
|Translated title of the contribution||Contents of four active flavonoids in different species of buckwheat|
|Number of pages||7|
|Journal||Chinese Journal of Applied and Environmental Biology|
|Publication status||Published - 25 Jun 2015|