LPS down-regulated α-syn mRNA but protein levels remained unchanged; TNFα caused mRNA down-regulation but up-regulation of protein, and des-Arg9-Bradykinin increased both, α-syn mRNA and protein. Given these results, the regulation of α-syn expression was addressed. In different cell types, three coding region splice variants and two α-syn 5’UTR splice variants are described. In this thesis I have identified a new splice variant in hMoDC (ex1”) and performed 5'RACE to determine the transcriptional start site (TSS). It is now clear that the TSS is positioned further upstream than previously published. Furthermore, the expression level of specific α-syn 5'UTR splice variants in hMoDC depends on the pro-inflammatory stimuli applied. For example, TNFα (despite downregulating α-syn mRNA expression generically) up-regulated the expression of the new novel ex1” splice variant which correlated with increased expression of α-syn protein.
|Qualification||Doctor of Philosophy|
|Publication status||Unpublished - 2008|