TY - JOUR
T1 - α-Glucosidase Inhibitors from the Bark Extract of Ethno-Antidiabetic Ceriops tagal (Perr.) C.B. Rob
AU - Canusa, Bernard S.
AU - Lawag, Ivan
AU - Villaflores, Oliver B.
AU - Wagh, Vitthal D.
AU - Korinek, Michal
AU - Tsai, Yi-Hong
AU - Chang, Fang-Rong
AU - Aguinaldo, Alicia M.
PY - 2022
Y1 - 2022
N2 - The bark of Ceriops tagal has been used in the Philippines for folk treatment of diabetes mellitus (DM). Through an α-glucosidase inhibition assay guided isolation, constituents from the detannified methanolic bark extract were isolated and identified to be methyl 2,4-dihydroxy-3,6-
dimethyl benzoate (1), isopimar-7-ene-15S,16-diol (2), betulinic acid (3), lupeol (4), and methyl
2,4-dihydroxy-6-methyl benzoate (5). Compounds 3 (IC50 = 5.31 μM) and 4 (IC50 = 55.84 μM)showed a more potent activity against α-glucosidase than the positive control, 1-deoxynojirimycin (IC50 = 79.16 μM). This is the first report on the presence of the weakly active compounds (1,
2, and 5) (IC50 > 75 μM) in the genus Ceriops and the first report on the biological activity of Compound 2. Enzyme kinetic studies suggested that betulinic acid is an uncompetitive type of inhibitor, with lupeol as a non-competitive type. This study provides evidence on the possible mechanism of the antidiabetic property of C. tagal associated with its traditional use. It supports the possible utilization of the bark for development to prevent or treat diabetes
AB - The bark of Ceriops tagal has been used in the Philippines for folk treatment of diabetes mellitus (DM). Through an α-glucosidase inhibition assay guided isolation, constituents from the detannified methanolic bark extract were isolated and identified to be methyl 2,4-dihydroxy-3,6-
dimethyl benzoate (1), isopimar-7-ene-15S,16-diol (2), betulinic acid (3), lupeol (4), and methyl
2,4-dihydroxy-6-methyl benzoate (5). Compounds 3 (IC50 = 5.31 μM) and 4 (IC50 = 55.84 μM)showed a more potent activity against α-glucosidase than the positive control, 1-deoxynojirimycin (IC50 = 79.16 μM). This is the first report on the presence of the weakly active compounds (1,
2, and 5) (IC50 > 75 μM) in the genus Ceriops and the first report on the biological activity of Compound 2. Enzyme kinetic studies suggested that betulinic acid is an uncompetitive type of inhibitor, with lupeol as a non-competitive type. This study provides evidence on the possible mechanism of the antidiabetic property of C. tagal associated with its traditional use. It supports the possible utilization of the bark for development to prevent or treat diabetes
UR - http://www.scopus.com/inward/record.url?scp=85140728161&partnerID=8YFLogxK
UR - https://philjournalsci.dost.gov.ph/publication/special-issues/marine-botany/107-vol-151-no-s1-marine-botany/1491-glucosidase-inhibitors-from-the-bark-extract-of-ethno-antidiabetic-ceriops-tagal-perr-c-b-rob
U2 - 10.56899/151.S1.03
DO - 10.56899/151.S1.03
M3 - Article
SN - 0031-7683
VL - 151
SP - 25
EP - 60
JO - Philippine Journal of Science
JF - Philippine Journal of Science
IS - S1
ER -
