Robert Tuckey

Associate Professor, BSc PhD W.Aust.

  • The University of Western Australia (M310), 35 Stirling Highway,

    6009 Perth

    Australia

  • 4006 Citations
  • 39 h-Index
1992 …2022
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Research Output 1992 2019

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Article
6 Citations (Scopus)

1 alpha,20S-Dihydroxyvitamin D-3 Interacts with Vitamin D Receptor: Crystal Structure and Route of Chemical Synthesis

Lin, Z., Chen, H., Belorusova, A. Y., Bollinger, J. C., Tang, E. K. Y., Janjetovic, Z., Kim, T-K., Wu, Z., Miller, D. D., Slominski, A. T., Postlethwaite, A. E., Tuckey, R. C., Rochel, N. & Li, W., 31 Aug 2017, In : Scientific Reports. 7, 10 p., 10193.

Research output: Contribution to journalArticle

Open Access
60 Citations (Scopus)

20,23-dihydroxyvitamin D3, novel P450scc product, stimulates differentiation and inhibits proliferation and NF-κB activity in human keratinocytes

Janjetovic, Z., Tuckey, R., Nguyen, M., Thorpe, E. M. & Slominski, A. T., 2010, In : Journal of Cellular Physiology. 223, p. 36-48

Research output: Contribution to journalArticle

81 Citations (Scopus)

20-Hydroxycholecalciferol, Product of Vitamin D3 Hydroxylation by P450scc, Decreases NF-kB Activity by Increasing IkBa Levels in Human Keratinocytes

Janjetovic, Z., Zmijewski, M. A., Tuckey, R., Deleon, D. A., Nguyen, M., Pfeffer, L. M. & Slominski, A. T., 2009, In : P L o S One. 4, 6, p. Article number e5988, 12pp

Research output: Contribution to journalArticle

Open Access
hydroxycholecalciferols
Hydroxylation
Hydroxycholecalciferols
cholecalciferol
Cholecalciferol
70 Citations (Scopus)

20-Hydroxyvitamin D2 is a noncalcemic analog of vitamin D with potent antiproliferative and prodifferentiation activities in normal and malignant cells

Slominski, A. T., Kim, T-K., Janjetovic, Z., Tuckey, R., Bieniek, R., Yue, J., Li, W., Chen, J., Nguyen, M., Tang, E., Miller, D., Chen, T. C. & Holick, M., 2011, In : American Journal of Physiology-Cell Physiology. 300, p. C526 - C541

Research output: Contribution to journalArticle

79 Citations (Scopus)

20-Hydroxyvitamin D3, a Product of Vitamin D3 Hydroxylation by Cytochrome P450scc, Stimulates Keratinocyte Differentiation

Zbytek, B., Janjetovic, Z., Tuckey, R., Zmijewski, M. A., Sweatman, T. W., Jones, E., Nguyen, M. & Slominski, A. T., 2008, In : Journal of Investigative Dermatology. 128, p. 2271-2280

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Hydroxylation
Cholecalciferol
Keratinocytes
Metabolism
43 Citations (Scopus)

20-Hydroxyvitamin D3 inhibits proliferation of cancer cells with high efficacy while being non-toxic

Wang, J., Slominski, A., Tuckey, R., Janjetovic, Z., Kulkarni, A., Chen, J., Postlethwaite, A. E., Miller, D. & Li, W., 2012, In : Anticancer Research. 32, p. 739-746

Research output: Contribution to journalArticle

44 Citations (Scopus)

20S-hydroxyvitamin D3, noncalcemic product of CYP11A1 action on vitamin D3, exhibits potent antifibrogenic activity in vivo

Słomiński, A., Janjetovic, Z., Tuckey, R., Nguyen, M., Bhattacharya, K. G., Wang, J., Li, W., Jiao, Y., Gu, W., Brown, M. & Postlethwaite, A. E., 2013, In : Journal of Clinical Endocrinology and Metabolism. 98, 2, p. E298-E303

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Cholecalciferol
Fibroblasts
Hydroxycholecalciferols
Collagen
3 Citations (Scopus)

Active Site Structures of CYP11A1 in the Presence of Its Physiological Substrates and Alterations upon Binding of Adrenodoxin

Zhu, Q., Mak, P. J., Tuckey, R. C. & Kincaid, J. R., 31 Oct 2017, In : Biochemistry. 56, 43, p. 5786-5797 12 p.

Research output: Contribution to journalArticle

Adrenodoxin
Cholesterol Side-Chain Cleavage Enzyme
Carbon Monoxide
Catalytic Domain
Substrates
39 Citations (Scopus)

Acute Erythemal Ultraviolet Radiation Causes Systemic Immunosuppression in the Absence of Increased 25-Hydroxyvitamin D3 Levels in Male Mice

Gorman, S., Scott, N. M., Tan, D. H. W., Weeden, C. E., Tuckey, R., Bisley, J. L., Grimbaldeston, M. A. & Hart, P., 26 Sep 2012, In : PLoS One. 7, 9, p. e46006.1-12

Research output: Contribution to journalArticle

Open Access
25-hydroxycholecalciferol
Calcifediol
cholecalciferol
Cholecalciferol
immunosuppression
67 Citations (Scopus)

An alternative pathway of vitamin D2 metabolismCytochrome P450scc (CYP11A1)-mediated conversion to 20-hydroxyvitamin D2 and 17,20-dihydroxyvitamin D2

Slominski, A., Semak, I., Wortsman, J., Zjawiony, J., Li, W., Zbytek, B. & Tuckey, R., 2006, In : The FEBS Journal. 273, 13, p. 2891-2901

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Ergocalciferols
Hydroxycholecalciferols
Dihydroxycholecalciferols
Metabolites
18 Citations (Scopus)

A new steroidal 5,7-diene derivative, 3β-hydroxyandrosta-5,7-diene-17β-carboxylic acid, shows potent anti-proliferative activity

Kim, T. K., Chen, J. J., Li, W., Zjawiony, J., Miller, D., Janjetovic, Z., Tuckey, R. & Slominski, A., 2010, In : Steroids. 75, p. 230-239

Research output: Contribution to journalArticle

167 Citations (Scopus)

A novel pathway for sequential transformation of 7-dehydrocholesterol and expression of the P450scc system in mammalian skin

Slominski, A., Zjawiony, J., Wortsman, J., Semak, I., Stewart, J., Pisarchik, A., Sweatman, T., Marcos, J., Dunbar, C. & Tuckey, R., 2004, In : European Journal of Biochemistry. 271, 21, p. 4178-4188

Research output: Contribution to journalArticle

Skin
Pregnenolone
Mitochondria
Cholecalciferol
Derivatives
23 Citations (Scopus)

Antitumor effects of vitamin D analogs on hamster and mouse melanoma cell lines in relation to melanin pigmentation

Wasiewicz, T., Szyszka, P., Cichorek, M., Janjetovic, Z., Tuckey, R., Słomiński, A. T. & Zmijewski, M. A., 2015, In : International Journal of Molecular Sciences. 16, 4, p. 6645-6667

Research output: Contribution to journalArticle

Open Access
calciferol
Melanin
melanin
hamsters
Vitamins
9 Citations (Scopus)

A proposed molecular mechanism of high-dose vitamin D3 supplementation in prevention and treatment of preeclampsia

Zabul, P., Wozniak, M., Slominski, A. T., Preis, K., Gorska, M., Korozan, M., Wieruszewski, J., Zmijewski, M. A., Zabul, E., Tuckey, R., Kuban-Jankowska, A., Mickiewicz, W. & Knap, N., 2015, In : International Journal of Molecular Sciences. 16, 6, p. 13043-13064

Research output: Contribution to journalArticle

Open Access
Cholesterol Side-Chain Cleavage Enzyme
vitamins
Cholecalciferol
Vitamins
cytochromes
7 Citations (Scopus)

A simplification of the deuterium oxide dilution technique using FT-IR analysis of plasma, for estimating piglet milk intake

Glencross, B. D., Mullan, B. P., Tuckey, R. & Hartmann, P., 1997, In : Crop and Pasture Science. 48, p. 1099-1104

Research output: Contribution to journalArticle

deuterium oxide
milk consumption
piglets
sublimation
litters (young animals)
6 Citations (Scopus)

Bioactive forms of vitamin D selectively stimulate the skin analog of the hypothalamus-pituitary-adrenal axis in human epidermal keratinocytes

Wierzbicka, J. M., Żmijewski, M. A., Piotrowska, A., Nedoszytko, B., Lange, M., Tuckey, R. & Slominski, A. T., 2016, In : Molecular and Cellular Endocrinology. 437, p. 312-322

Research output: Contribution to journalArticle

Cholecalciferol
Keratinocytes
Vitamin D
Hypothalamus
Skin

Biochemistry in Western Australia

Newsholme, P., Hartmann, P., Tuckey, R. & Bond, C., 2015, In : Biochemist. 37, 6, p. 32-34

Research output: Contribution to journalArticle

1 Citation (Scopus)

Catalytic properties of 25-hydroxyvitamin D3 3-epimerase in rat and human liver microsomes

Tuckey, R. C., Tang, E. K. Y., Maresse, S. R. & Delaney, D. S., 15 May 2019, In : Archives of Biochemistry and Biophysics. 666, p. 16-21 6 p.

Research output: Contribution to journalArticle

Racemases and Epimerases
Calcifediol
Liver Microsomes
Liver
NAD
39 Citations (Scopus)
19 Citations (Scopus)

Characterization of a new pathway that activates lumisterol in vivo to biologically active hydroxylumisterols

Slominski, A. T., Kim, T-K., Hobrath, J. V., Janjetovic, Z., Oak, A. S. W., Postlethwaite, A., Lin, Z., Li, W., Takeda, Y., Jetten, A. M. & Tuckey, R. C., 12 Sep 2017, In : Scientific Reports. 7, 1, 17 p., 11434.

Research output: Contribution to journalArticle

Open Access
12 Citations (Scopus)

Chemical Synthesis and Biological Activities of 20S,24S/R-Dihydroxyvitamin D3 Epimers and Their 1α-Hydroxyl Derivatives

Lin, Z., Marepally, S. R., Ma, D., Myers, L. K., Postlethwaite, A. E., Tuckey, R., Cheng, C., Kim, T. K., Yue, J., Slominski, A. T., Miller, D. D. & Li, W., 2015, In : Journal of Medicinal Chemistry. 58, 19, p. 7881-7887

Research output: Contribution to journalArticle

Hydroxyl Radical
25-Hydroxyvitamin D3 1-alpha-Hydroxylase
Calcitriol Receptors
Cholecalciferol
Hydroxylation
36 Citations (Scopus)

Chemical synthesis of 20S-hydroxyvitamin D3, which shows antiproliferative activity

Li, W., Chen, J., Janjetovic, Z., Kim, T. K., Sweatman, T., Lu, Y., Zjawiony, J., Tuckey, R., Miller, D. & Slominski, A., 2010, In : Steroids. 75, p. 926-935

Research output: Contribution to journalArticle

27 Citations (Scopus)

Classical and non-classical metabolic transformation of vitamin D in dermal fibroblasts

Slominski, A. T., Kim, T. K., Li, W. & Tuckey, R., 2016, In : Experimental Dermatology. 25, 3, p. 231-232

Research output: Contribution to journalArticle

39 Citations (Scopus)

Correlation between secosteroid-induced vitamin D receptor activity in melanoma cells and computer-modeled receptor binding strength

Kim, T-K., Wang, J., Janjetovic, Z., Chen, J., Tuckey, R., Nguyen, M., Tang, E. K. Y., Miller, D., Li, W. & Slominski, A. T., 2012, In : Molecular and Cellular Endocrinology. 361, p. 143-152

Research output: Contribution to journalArticle

43 Citations (Scopus)

Cutaneous glucocorticosteroidogenesis: Securing local homeostasis and the skin integrity

Słomiński, A. T., Manna, P. R. & Tuckey, R., 2014, In : Experimental Dermatology. 23, 6, p. 369-374

Research output: Contribution to journalArticle

Skin
Homeostasis
Steroids
Ultraviolet Rays
Cyclic AMP-Dependent Protein Kinases
107 Citations (Scopus)

Cutaneous hypothalamic-pituitary-adrenal axis homolog: regulation by ultraviolet radiation

Skobowiat, C., Dowdy, J. C., Sayre, R. M., Tuckey, R. & Slominski, A., 2011, In : AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM. 301, p. E484-E493

Research output: Contribution to journalArticle

Corticotropin-Releasing Hormone
Cholesterol Side-Chain Cleavage Enzyme
Radiation
Pro-Opiomelanocortin
Skin
23 Citations (Scopus)

CYP11A1 in skin: An alternative route to photoprotection by vitamin D compounds

Tongkao-On, W., Carter, S. E., Reeve, V. E., Dixon, K. M., Gordon-Thomson, C., Halliday, G. M., Tuckey, R. & Mason, R. S., Apr 2015, In : Journal of Steroid Biochemistry and Molecular Biology. 148, p. 72-78

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Vitamin D
Ultraviolet radiation
Skin
Radiation
23 Citations (Scopus)

CYP24A1 Expression Inversely Correlates with Melanoma Progression: Clinic-Pathological Studies

Brozyna, A. A., Jochymski, C., Janjetovic, Z., Józwicki, W., Tuckey, R. & Slominski, A. T., 2014, In : International Journal of Molecular Sciences (Online). 15, p. 19000-19017

Research output: Contribution to journalArticle

Open Access
4 Citations (Scopus)

CYP27A1 acts on the pre-vitamin D3 photoproduct, lumisterol, producing biologically active hydroxy-metabolites

Tuckey, R. C., Li, W., Ma, D., Cheng, C. Y. S., Wang, K. M., Kim, T-K., Jeayeng, S. & Slominski, A. T., Jul 2018, In : The Journal of Steroid Biochemistry and Molecular Biology. 181, p. 1-10 10 p.

Research output: Contribution to journalArticle

Ergosterol
Hydroxylation
Mitochondria
Cholecalciferol
Cell proliferation
1 Citation (Scopus)

Cytochrome P-450SCC activity and substrate supply in human placental trophoblasts

Tuckey, R., Kostadinovic, Z. & Cameron, K. J., 1994, In : Molecular and Cellular Endocrinology. 105, p. 103-109

Research output: Contribution to journalArticle

47 Citations (Scopus)

Cytochrome P450scc-dependent metabolism of 7-dehydrocholesterol in placenta and epidermal keratinocytes

Slominski, A. T., Kim, T-K., Chen, J., Nguyen, M., Li, W., Yates, C. R., Sweatman, T., Janjetovic, Z. & Tuckey, R., 2012, In : International Journal of Biochemistry & Cell Biology. 44, p. 2003– 2018

Research output: Contribution to journalArticle

57 Citations (Scopus)

Cytochromes P450 and skin cancer: Role of local endocrine pathways

Słomiński, A. T., Zmijewski, M. A., Semak, I. V., Zbytek, B., Pisarchik, A. V., Li, W., Zjawiony, J. K. & Tuckey, R., 2014, In : Anti-Cancer Agents in Medicinal Chemistry. 14, 1, p. 77-96

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Skin Neoplasms
Cytochrome P-450 Enzyme System
Skin
Hydroxylation
49 Citations (Scopus)

Cytosolic NADP+-dependent Isocitrate Dehydrogenase

Jennings, G. J., Sechi, S., Stevenson, P. M., Tuckey, R., Parmelee, D. & Mcalister-Henn, L., 1994, In : The Journal of Biological Chemistry. 269, 37, p. 23128-23134

Research output: Contribution to journalArticle

22 Citations (Scopus)

Design, synthesis, and biological action of 20 R -hydroxyvitamin D3

Lu, Y., Chen, J., Janjetovic, Z., Michaels, P., Tang, E. K. Y., Wang, J., Tuckey, R., Slominski, A. T., Li, W. & Miller, D. D., 2012, In : Journal of Medicinal Chemistry. 55, p. 3573−3577

Research output: Contribution to journalArticle

4 Citations (Scopus)

Design, Synthesis and Biological Activities of Novel Gemini 20S-Hydroxyvitamin D-3 Analogs

Lin, Z., Marepally, S. R., Kim, T., Janjetovic, Z., Oak, A. S. W., Postlethwaite, A. E., Myers, L. K., Tuckey, R. C., Slominski, A. T., Miller, D. D. & Li, W., 2016, In : Anticancer Research. 36, 3, p. 877-886 10 p.

Research output: Contribution to journalArticle

Calcitriol Receptors
Hydroxycholecalciferols
Cholesterol Side-Chain Cleavage Enzyme
Anti-Inflammatory Agents
Transient Receptor Potential Channels
85 Citations (Scopus)

Detection of novel CYP11A1-derived secosteroids in the human epidermis and serum and pig adrenal gland

Slominski, A. T., Kim, T. K., Li, W., Postlethwaite, A., Tieu, E., Tang, E. & Tuckey, R., 2015, In : Scientific Reports. 5, p. 14875

Research output: Contribution to journalArticle

Open Access
Secosteroids
Cholesterol Side-Chain Cleavage Enzyme
Bioactivity
Cholecalciferol
Metabolism
17 Citations (Scopus)

Differential and Overlapping Effects of 20,23(OH)₂D3 and 1,25(OH)₂D3 on Gene Expression in Human Epidermal Keratinocytes: Identification of AhR as an Alternative Receptor for 20,23(OH)₂D3

Slominski, A. T., Kim, T. K., Janjetovic, Z., Brożyna, A. A., Żmijewski, M. A., Xu, H., Sutter, T. R., Tuckey, R. C., Jetten, A. M. & Crossman, D. K., 8 Oct 2018, In : International Journal of Molecular Sciences. 19, 10

Research output: Contribution to journalArticle

Open Access
gene expression
Vitamins
Keratinocytes
Gene expression
vitamins
22 Citations (Scopus)

Differential antitumor effects of vitamin D analogues on colorectal carcinoma in culture

Wierzbicka, J. M., Binek, A., Ahrends, T., Nowac Ka, J. D., Szydłowska, A., Wąsiewic Z, T., Wierzbicki, P. M., Sądej, R., Tuckey, R., Slominski, A. T., Chybicki, J., Adrych, K., Kmieć, Z. & Zmijewski, M. A., 2015, In : International Journal of Oncology. 47, 3, p. 1084-1096

Research output: Contribution to journalArticle

Cholecalciferol
Vitamin D
Colorectal Neoplasms
Cell Line
Avitaminosis
166 Citations (Scopus)

Differential expression of HPA axis homolog in the skin

Slominski, A., Wortsman, J., Tuckey, R. & Paus, R., 2007, In : Molecular and Cellular Endocrinology. 265-266, p. 143-149

Research output: Contribution to journalArticle

Corticotropin-Releasing Hormone
Skin
Pro-Opiomelanocortin
Hydrocortisone
Melanocytes
16 Citations (Scopus)

Electrochemical behaviour of human adrenodoxin on a pyrolytic graphite electrode

Johnson, D., Norman, S., Tuckey, R. & Martin, L. L., 2003, In : Bioelectrochemistry. 59, 1-2, p. 41-47

Research output: Contribution to journalArticle

Adrenodoxin
Graphite electrodes
Graphite
pyrolytic graphite
Electrodes

Electron transfer to cytochrome P-450scc limits cholesterold-side-chain-cleavage activity in the human placenta

Tuckey, R., Woods, S. T. & Tajbakhsh, M., 1997, In : European Journal of Biochemistry:FEBS. 244, p. 835-839

Research output: Contribution to journalArticle

41 Citations (Scopus)

Endogenously produced nonclassical vitamin D hydroxy-metabolites act as “biased” agonists on VDR and inverse agonists on RORα and RORγ

Slominski, A. T., Kim, T. K., Hobrath, J. V., Oak, A. S. W., Tang, E. K. Y., Tieu, E. W., Li, W., Tuckey, R. C. & Jetten, A. M., 1 Oct 2017, In : Journal of Steroid Biochemistry and Molecular Biology. 173, p. 42-56 15 p.

Research output: Contribution to journalArticle

Metabolites
Vitamin D
Secosteroids
Molecular modeling
Ligands
45 Citations (Scopus)

Enzymatic Metabolism of Ergosterol by Cytochrome P450scc to Biologically Active 17α,24-Dihydroxyergosterol

Slominski, A., Semak, I., Zjawiony, J., Wortsman, J., Gandy, M., Li, J., Zbytek, B., Li, W. & Tuckey, R., 2005, In : Chemistry & Biology. 12, 8, p. 931-939

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Ergosterol
Metabolism
Hydroxyl Radical
Cholesterol
14 Citations (Scopus)

Enzymatic properties of vesicle-reconstituted human cytochrome P450SCC (CY11A1)

Kisselev, P., Tuckey, R., Woods, S. T., Triantopoulos, T. & Schwarz, D., 1999, In : European Journal of Biochemistry. 260, p. 768-773

Research output: Contribution to journalArticle

50 Citations (Scopus)

Expression of Catalytically Active Human Cytochrome P450scc in Escherichia coli and Mutagenesis of Isoleucine-462

Woods, S. T., Sadleir, J., Downs, T., Triantopoulos, T., Headlam, M. J. & Tuckey, R., 1998, In : Archives of Biochemistry and Biophysics. 353, 1, p. 109-115

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Mutagenesis
Isoleucine
Escherichia coli
Adrenodoxin
10 Citations (Scopus)

Expression of human CYP27B1 in Escherichia coli and characterization in phospholipid vesicles

Tang, E. K. Y., Tieu, E. & Tuckey, R., 2012, In : FEBS Journal. 279, p. 3749

Research output: Contribution to journalArticle

58 Citations (Scopus)

High basal NF-ĸB activity in nonpigmented melanoma cells is associated with an enhanced sensitivity to vitamin D3 derivatives

Janjetovic, Z., Brozyna, A. A., Tuckey, R., Kim, T. K., Nguyen, M., Jozwicki, W., Pfeffer, S. R., Pfeffer, L. M. & Slominski, A. T., 2011, In : British Journal of Cancer. 105, p. 1874 – 1884

Research output: Contribution to journalArticle

Open Access
17 Citations (Scopus)

Human cytochrome P450scc (CYP11A1) Catalyzes Epoxide Formation with Ergosterol

Tuckey, R., Nguyen, M., Chen, J., Slominski, A. T., Baldisseri, D. M., Tieu, E., Zjawiony, J. K. & Li, W., 2012, In : Drug Metabolism and Disposition. 40, 3, p. 436–444

Research output: Contribution to journalArticle

Human Placental Cholesterol Side-Chain Cleavage : Enzymatic Synthesis of (22R)-20a,22-Dihydroxycholesterol

Tuckey, R. & Cameron, K. J., 1993, In : Steroids. 58, p. 230-233

Research output: Contribution to journalArticle