Robert Tuckey

Associate Professor, BSc PhD W.Aust.

  • The University of Western Australia (M310), 35 Stirling Highway,

    6009 Perth

    Australia

  • 4008 Citations
  • 39 h-Index
1992 …2022
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Research Output 1992 2019

2013
22 Citations (Scopus)

Hydroxylation of CYP11A1-derived products of vitamin D3 metabolism by human and Mouse CYP27B1

Tang, E. K. Y., Chen, J., Janjetovic, Z., Tieu, E., Słomiński, A. T., Li, W. & Tuckey, R., 2013, In : Drug Metabolism and Disposition. 41, 5, p. 1112-1124

Research output: Contribution to journalArticle

25-Hydroxyvitamin D3 1-alpha-Hydroxylase
Cholesterol Side-Chain Cleavage Enzyme
Cholecalciferol
Hydroxylation
Secosteroids

Novel metabolism of vitamin D in human epidermal keratinocytes and epithelial colon cells

Kim, T., Lin, Z., Tuckey, R., Tang, E., Li, W. & Slominski, A. T., 2013, In : Journal of Investigative Dermatology. 133, p. S116-S116

Research output: Contribution to journalReview article

35 Citations (Scopus)

Novel vitamin D photoproducts and their precursors in the skin

Słomiński, A. T., Kim, T., Zmijewski, M. A., Janjetovic, Z., Li, W., Chen, J., Kusniatsova, E. I., Semak, I. V., Postlethwaite, A. E., Miller, D. D., Zjawiony, J. K. & Tuckey, R., 2013, In : Dermato-Endocrinology. 5, 1, p. 7-19

Research output: Contribution to journalReview article

Cholesterol Side-Chain Cleavage Enzyme
Secosteroids
Vitamin D
Ergocalciferols
Cholecalciferol
176 Citations (Scopus)

Steroidogenesis in the skin: Implications for local immune functions

Słomiński, A. T., Zbytek, B., Nikolakis, G., Manna, P. R., Skobowiat, C., Zmijewski, M. A., Li, W., Janjetovic, Z., Postlethwaite, A. E., Zouboulis, C. C. Z. & Tuckey, R., 2013, In : Journal of Steroid Biochemistry and Molecular Biology. 137, p. 107-123

Research output: Contribution to journalArticle

Skin
Glucocorticoids
Androgens
Estrogens
Steroids
2012
43 Citations (Scopus)

20-Hydroxyvitamin D3 inhibits proliferation of cancer cells with high efficacy while being non-toxic

Wang, J., Slominski, A., Tuckey, R., Janjetovic, Z., Kulkarni, A., Chen, J., Postlethwaite, A. E., Miller, D. & Li, W., 2012, In : Anticancer Research. 32, p. 739-746

Research output: Contribution to journalArticle

39 Citations (Scopus)

Acute Erythemal Ultraviolet Radiation Causes Systemic Immunosuppression in the Absence of Increased 25-Hydroxyvitamin D3 Levels in Male Mice

Gorman, S., Scott, N. M., Tan, D. H. W., Weeden, C. E., Tuckey, R., Bisley, J. L., Grimbaldeston, M. A. & Hart, P., 26 Sep 2012, In : PLoS One. 7, 9, p. e46006.1-12

Research output: Contribution to journalArticle

Open Access
25-hydroxycholecalciferol
Calcifediol
cholecalciferol
Cholecalciferol
immunosuppression
39 Citations (Scopus)

Correlation between secosteroid-induced vitamin D receptor activity in melanoma cells and computer-modeled receptor binding strength

Kim, T-K., Wang, J., Janjetovic, Z., Chen, J., Tuckey, R., Nguyen, M., Tang, E. K. Y., Miller, D., Li, W. & Slominski, A. T., 2012, In : Molecular and Cellular Endocrinology. 361, p. 143-152

Research output: Contribution to journalArticle

47 Citations (Scopus)

Cytochrome P450scc-dependent metabolism of 7-dehydrocholesterol in placenta and epidermal keratinocytes

Slominski, A. T., Kim, T-K., Chen, J., Nguyen, M., Li, W., Yates, C. R., Sweatman, T., Janjetovic, Z. & Tuckey, R., 2012, In : International Journal of Biochemistry & Cell Biology. 44, p. 2003– 2018

Research output: Contribution to journalArticle

22 Citations (Scopus)

Design, synthesis, and biological action of 20 R -hydroxyvitamin D3

Lu, Y., Chen, J., Janjetovic, Z., Michaels, P., Tang, E. K. Y., Wang, J., Tuckey, R., Slominski, A. T., Li, W. & Miller, D. D., 2012, In : Journal of Medicinal Chemistry. 55, p. 3573−3577

Research output: Contribution to journalArticle

10 Citations (Scopus)

Expression of human CYP27B1 in Escherichia coli and characterization in phospholipid vesicles

Tang, E. K. Y., Tieu, E. & Tuckey, R., 2012, In : FEBS Journal. 279, p. 3749

Research output: Contribution to journalArticle

17 Citations (Scopus)

Human cytochrome P450scc (CYP11A1) Catalyzes Epoxide Formation with Ergosterol

Tuckey, R., Nguyen, M., Chen, J., Slominski, A. T., Baldisseri, D. M., Tieu, E., Zjawiony, J. K. & Li, W., 2012, In : Drug Metabolism and Disposition. 40, 3, p. 436–444

Research output: Contribution to journalArticle

133 Citations (Scopus)

In vivo evidence for a novel pathway of vitamin D3 metabolism initiated by P450scc and modified by CYP27B1

Slominski, A. T., Kim, T-K., Shehabi, H. Z., Semak, I., Tang, E. K. Y., Nguyen, M., Benson, H. A. E., Korik, E., Janjetovic, Z., Chen, J., Yates, C. R., Postlethwaite, A., Li, W. & Tuckey, R., 2012, In : FASEB Journal. 26, p. 3901

Research output: Contribution to journalArticle

28 Citations (Scopus)

Metabolism of cholesterol, vitamin D3 and 20-hydroxyvitamin D3 incorporated into phospholipid vesicles by human CYP27A1

Tieu, E., Li, W., Chen, J., Baldisseri, D. M., Slominski, A. T. & Tuckey, R., 2012, In : Journal of Steroid Biochemistry and Molecular Biology. 129, p. 163– 171

Research output: Contribution to journalArticle

48 Citations (Scopus)

Novel vitamin D hydroxyderivatives inhibit melanoma growth and show differential effects on normal melanocytes

Slominski, A. T., Janjetovic, Z., Kim, T-K., Wright, A. C., Grese, L. N., Riney, S. J., Nguyen, M. & Tuckey, R., 2012, In : Anticancer Research. 32, p. 3733-3742

Research output: Contribution to journalArticle

32 Citations (Scopus)

Rat CYP24A1 acts on 20-hydroxyvitamin D3 producing hydroxylated products with increased biological activity

Tieu, E., Tang, E. K. Y., Chen, J., Li, W., Nguyen, M., Janjetovic, Z., Slominski, A. & Tuckey, R., 2012, In : Biochemical Pharmacology. 84, p. 1696–1704

Research output: Contribution to journalArticle

2011
70 Citations (Scopus)

20-Hydroxyvitamin D2 is a noncalcemic analog of vitamin D with potent antiproliferative and prodifferentiation activities in normal and malignant cells

Slominski, A. T., Kim, T-K., Janjetovic, Z., Tuckey, R., Bieniek, R., Yue, J., Li, W., Chen, J., Nguyen, M., Tang, E., Miller, D., Chen, T. C. & Holick, M., 2011, In : American Journal of Physiology-Cell Physiology. 300, p. C526 - C541

Research output: Contribution to journalArticle

107 Citations (Scopus)

Cutaneous hypothalamic-pituitary-adrenal axis homolog: regulation by ultraviolet radiation

Skobowiat, C., Dowdy, J. C., Sayre, R. M., Tuckey, R. & Slominski, A., 2011, In : AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM. 301, p. E484-E493

Research output: Contribution to journalArticle

Corticotropin-Releasing Hormone
Cholesterol Side-Chain Cleavage Enzyme
Radiation
Pro-Opiomelanocortin
Skin
58 Citations (Scopus)

High basal NF-ĸB activity in nonpigmented melanoma cells is associated with an enhanced sensitivity to vitamin D3 derivatives

Janjetovic, Z., Brozyna, A. A., Tuckey, R., Kim, T. K., Nguyen, M., Jozwicki, W., Pfeffer, S. R., Pfeffer, L. M. & Slominski, A. T., 2011, In : British Journal of Cancer. 105, p. 1874 – 1884

Research output: Contribution to journalArticle

Open Access
53 Citations (Scopus)

Production of 22-hydroxy metabolites of vitamin D3 by cytochrome P450scc (CYP11A1) and analysis of their biological activities on skin cells

Tuckey, R., Li, W., Shehabi, H. Z., Janjetovic, Z., Nguyen, M., Kim, T-K., Chen, J., Howell, D. E., Benson, H. A. E., Sweatman, T., Baldisseri, D. M. & Slominski, A., 2011, In : Drug Metabolism and Disposition. 39, 9, p. 1577–1588

Research output: Contribution to journalArticle

30 Citations (Scopus)

Vitamin D Analogs 17,20S(OH)2pD and 17,20R(OH)2pD Are Noncalcemic and Exhibit Antifibrotic Activity

Slominski, A. T., Li, W., Bhattacharya, S. K., Smith, R. A., Johnson, P. L., Chen, J., Nelson, K. E., Tuckey, R., Miller, D., Jiao, Y., Gu, W. & Postlethwaite, A. E., 2011, In : Journal of Investigative Dermatology. 131, p. 1167–1169

Research output: Contribution to journalLetter

Open Access
2010
60 Citations (Scopus)

20,23-dihydroxyvitamin D3, novel P450scc product, stimulates differentiation and inhibits proliferation and NF-κB activity in human keratinocytes

Janjetovic, Z., Tuckey, R., Nguyen, M., Thorpe, E. M. & Slominski, A. T., 2010, In : Journal of Cellular Physiology. 223, p. 36-48

Research output: Contribution to journalArticle

18 Citations (Scopus)

A new steroidal 5,7-diene derivative, 3β-hydroxyandrosta-5,7-diene-17β-carboxylic acid, shows potent anti-proliferative activity

Kim, T. K., Chen, J. J., Li, W., Zjawiony, J., Miller, D., Janjetovic, Z., Tuckey, R. & Slominski, A., 2010, In : Steroids. 75, p. 230-239

Research output: Contribution to journalArticle

36 Citations (Scopus)

Chemical synthesis of 20S-hydroxyvitamin D3, which shows antiproliferative activity

Li, W., Chen, J., Janjetovic, Z., Kim, T. K., Sweatman, T., Lu, Y., Zjawiony, J., Tuckey, R., Miller, D. & Slominski, A., 2010, In : Steroids. 75, p. 926-935

Research output: Contribution to journalArticle

30 Citations (Scopus)
88 Citations (Scopus)

Products of Vitamin D3 or 7-Dehydrocholesterol Metabolism by Cytochrome P450scc Show Anti-Leukemia Effects, Having Low or Absent Calcemic Activity

Slominski, A. T., Janjetovic, Z., Fuller, B. E., Zmijewski, M. A., Tuckey, R., Nguyen, M., Sweatman, T., Li, W., Zjawiony, J., Miller, D., Chen, T. C., Lozanski, G. & Holick, M. F., 2010, In : PLoS One. 5, p. Article number e9907, 13pp

Research output: Contribution to journalArticle

Open Access
26 Citations (Scopus)
2009
81 Citations (Scopus)

20-Hydroxycholecalciferol, Product of Vitamin D3 Hydroxylation by P450scc, Decreases NF-kB Activity by Increasing IkBa Levels in Human Keratinocytes

Janjetovic, Z., Zmijewski, M. A., Tuckey, R., Deleon, D. A., Nguyen, M., Pfeffer, L. M. & Slominski, A. T., 2009, In : P L o S One. 4, 6, p. Article number e5988, 12pp

Research output: Contribution to journalArticle

Open Access
hydroxycholecalciferols
Hydroxylation
Hydroxycholecalciferols
cholecalciferol
Cholecalciferol
37 Citations (Scopus)

Metabolism of Vitamin D2 to 17,20,24-Trihydroxyvitamin D2 by Cytochrome P450scc (CYP11A1)

Nguyen, M., Slominski, A., Li, W., Ng, Y. R. & Tuckey, R., 2009, In : Drug Metabolism and Disposition: the biological fate of chemicals. 37, 4, p. 761-767

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Ergocalciferols
Cyclodextrins
Cholesterol
Hydroxylation
3 Citations (Scopus)

Role of CYP27A1 in progesterone metabolism in vitro and in vivo

Escher, G., Vogeli, I., Escher, R., Tuckey, R., Erickson, S., Krozowski, Z. & Frey, F. J., 2009, In : American journal of physiology : endocrinology and metabolism. 297, 4, p. E949-E955

Research output: Contribution to journalArticle

Progesterone
In Vitro Techniques
20-Hydroxysteroid Dehydrogenases
Knockout Mice
Adrenodoxin
59 Citations (Scopus)

Sequential Metabolism of 7-Dehydrocholesterol to Steroidal 5,7-Dienes in Adrenal Glands and Its Biological Implication in the Skin

Slominski, A. T., Zmijewski, M. A., Semak, I., Sweatman, T., Janjetovic, Z., Li, W., Zjawiony, J. K. & Tuckey, R., 2009, In : P L o S One. 4, 2, p. Article number e4309, 18pp

Research output: Contribution to journalArticle

Open Access
7-dehydrocholesterol
adrenal glands
Adrenal Glands
Metabolism
skin (animal)
2008

20-Hydroxycholecalciferol and 20,23-dihydroxycholecalciferol, products of vitamin D3 hydroxylation by P450-scc, decrease NF kappa B activity by increasing I kappa B alpha levels in normal human- and HaCaT-keratinocytes

Lanietovic, Z., Zmijewski, MA., Tuckey, R., Nguyen, M. & Slominski, AT., 2008, JOURNAL OF INVESTIGATIVE DERMATOLOGY. Vol. 128. p. S20-S20

Research output: Chapter in Book/Conference paperConference paper

79 Citations (Scopus)

20-Hydroxyvitamin D3, a Product of Vitamin D3 Hydroxylation by Cytochrome P450scc, Stimulates Keratinocyte Differentiation

Zbytek, B., Janjetovic, Z., Tuckey, R., Zmijewski, M. A., Sweatman, T. W., Jones, E., Nguyen, M. & Slominski, A. T., 2008, In : Journal of Investigative Dermatology. 128, p. 2271-2280

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Hydroxylation
Cholecalciferol
Keratinocytes
Metabolism
33 Citations (Scopus)

Kinetics of vitamin D3 metabolism by cytochrome P450scc (CYP11A1) in phospholipid vesicles and cylcodextrin

Tuckey, R., Nguyen, M. & Slominski, A., 2008, In : International Journal of Biochemistry & Cell Biology. 40, 11, p. 2619-2626

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Enzyme kinetics
Cholecalciferol
Metabolism
Phospholipids
34 Citations (Scopus)

Metabolism of 1α-hydroxyvitamin D3 by cytochrome P450scc to biologically active 1α,20-dihydroxyvitamin D3

Tuckey, R., Janjetovic, Z., Li, W., Nguyen, M., Zjawiony, J., Zmijewski, M. A. & Slominski, A., 2008, In : Journal of Steroid Biochemistry and Molecular Biology. 112, 4-5, p. 213-219

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Metabolism
Cholecalciferol
Keratinocytes
Calcitriol
77 Citations (Scopus)

Pathways and products for the metabolism of vitamin D3 by cytochrome P450scc

Tuckey, R., Li, W., Zjawiony, J. K., Zmijewski, M. A., Nguyen, M., Sweatman, T., Miller, D. & Slominski, A., 2008, In : The FEBS Journal. 275, 10, p. 2585-2596

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Cholecalciferol
Metabolism
Hydroxyl Radical
Nuclear magnetic resonance
2007
166 Citations (Scopus)

Differential expression of HPA axis homolog in the skin

Slominski, A., Wortsman, J., Tuckey, R. & Paus, R., 2007, In : Molecular and Cellular Endocrinology. 265-266, p. 143-149

Research output: Contribution to journalArticle

Corticotropin-Releasing Hormone
Skin
Pro-Opiomelanocortin
Hydrocortisone
Melanocytes
2006
67 Citations (Scopus)

An alternative pathway of vitamin D2 metabolismCytochrome P450scc (CYP11A1)-mediated conversion to 20-hydroxyvitamin D2 and 17,20-dihydroxyvitamin D2

Slominski, A., Semak, I., Wortsman, J., Zjawiony, J., Li, W., Zbytek, B. & Tuckey, R., 2006, In : The FEBS Journal. 273, 13, p. 2891-2901

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Ergocalciferols
Hydroxycholecalciferols
Dihydroxycholecalciferols
Metabolites
2005
45 Citations (Scopus)

Enzymatic Metabolism of Ergosterol by Cytochrome P450scc to Biologically Active 17α,24-Dihydroxyergosterol

Slominski, A., Semak, I., Zjawiony, J., Wortsman, J., Gandy, M., Li, J., Zbytek, B., Li, W. & Tuckey, R., 2005, In : Chemistry & Biology. 12, 8, p. 931-939

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Ergosterol
Metabolism
Hydroxyl Radical
Cholesterol
153 Citations (Scopus)

Progesterone Synthesis by the Human Placenta

Tuckey, R., 2005, In : Placenta. 26, 4, p. 273-281

Research output: Contribution to journalArticle

Placenta
Progesterone
Ferredoxin-NADP Reductase
Pregnenolone
Adrenodoxin
96 Citations (Scopus)

The cytochrome P450scc system opens an alternate pathway of vitamin D3 metabolism

Slominski, A., Semak, I., Zjawiony, J., Wortsman, J., Wei, L., Szczesniewski, A. & Tuckey, R., 2005, In : The FEBS Journal. 272, 16, p. 4080-4090

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Cholecalciferol
Open systems
Metabolism
Mitochondria
2004
167 Citations (Scopus)

A novel pathway for sequential transformation of 7-dehydrocholesterol and expression of the P450scc system in mammalian skin

Slominski, A., Zjawiony, J., Wortsman, J., Semak, I., Stewart, J., Pisarchik, A., Sweatman, T., Marcos, J., Dunbar, C. & Tuckey, R., 2004, In : European Journal of Biochemistry. 271, 21, p. 4178-4188

Research output: Contribution to journalArticle

Skin
Pregnenolone
Mitochondria
Cholecalciferol
Derivatives
43 Citations (Scopus)

Molten Globule Structure and Steroidogenic Activity of N-218 MLN64 in Human Placental Mitochondria

Tuckey, R., Bose, H., Czerwionka, I. & Miller, W. L., 2004, In : Endocrinology. 145, 4, p. 1700-1707

Research output: Contribution to journalArticle

Mitochondria
Cholesterol
Placenta
Pregnenolone
Mitochondrial Membranes
2003
16 Citations (Scopus)

Electrochemical behaviour of human adrenodoxin on a pyrolytic graphite electrode

Johnson, D., Norman, S., Tuckey, R. & Martin, L. L., 2003, In : Bioelectrochemistry. 59, 1-2, p. 41-47

Research output: Contribution to journalArticle

Adrenodoxin
Graphite electrodes
Graphite
pyrolytic graphite
Electrodes
53 Citations (Scopus)

The F-G loop region of cytochrome P450scc (CYP11A1) interacts with the phospholipid membrane

Headlam, M. J., Wilce, M. C. & Tuckey, R., 2003, In : Biochimica et Biophysica Acta - Biomembranes. 1617, 1-2, p. 96-108

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Phospholipids
Membranes
ethylenediamine
Association reactions
4 Citations (Scopus)

The effect of glycerol on cytochrome P450scc (CYP11A1) spin state, activity, and hydration

Headlam, M. J. & Tuckey, R., 2002, In : Archives of Biochemistry and Biophysics. 407, p. 95-102

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Hydration
Glycerol
Osmotic Pressure
Adrenodoxin
49 Citations (Scopus)

Transfer of Cholesterol between Phospholipid Vesicles Mediated by the Steroidogenic Acute Regulatory Protein (StAR)

Tuckey, R., Headlam, M. J., Bose, H. S. & Miller, W. L., 2002, In : Journal of Biological Chemistry. 277, 49, December 06, p. 47123-47128

Research output: Contribution to journalArticle

Phospholipids
Cholesterol
Pregnenolone
Mitochondrial Membranes
Cytochromes
2001
15 Citations (Scopus)
Adrenodoxin
Cholesterol Side-Chain Cleavage Enzyme
Mitochondria
Placenta
Ferredoxin-NADP Reductase
1999

Adrenodoxin reductase limits cytochrome P450scc activity in the human placenta

Tuckey, R., Mckinley, A. & Sadleir, J., 1999, 52nd Yamada Conference : International Symposium on Molecular Steroidogenesis. Okamoto, M. (ed.). Nara, Japan ed. Tokyo, Japan: Universal Academy Press, Inc., Vol. Frontiers Science Series No. 2. p. 119-120

Research output: Chapter in Book/Conference paperConference paper