Robert Tuckey

Associate Professor

  • The University of Western Australia (M310), 35 Stirling Highway,

    6009 Crawley

    Australia

  • 3214 Citations
  • 35 h-Index
1992 …2022

Research Output 1992 2018

2018
1 Citations

Investigation of 20S-hydroxyVitamin D3 analogs and their 1α-OH derivatives as potent Vitamin D receptor agonists with anti-inflammatory activities

Lin, Z., Marepally, S. R., Goh, E. S. Y., Cheng, C. Y. S., Janjetovic, Z., Kim, T. K., Miller, D. D., Postlethwaite, A. E., Slominski, A. T., Tuckey, R. C., Peluso-Iltis, C., Rochel, N. & Li, W. 1 Dec 2018 In : Scientific Reports. 8, 1, 1478

Research output: Contribution to journalArticle

Open Access
Calcitriol Receptors
Hydroxycholecalciferols
25-Hydroxyvitamin D3 1-alpha-Hydroxylase
Anti-Inflammatory Agents
Hydroxylation

Properties of purified CYP2R1 in a reconstituted membrane environment and its 25-hydroxylation of 20-hydroxyvitamin D3

Cheng, C. Y. S., Kim, T. K., Jeayeng, S., Slominski, A. T. & Tuckey, R. C. 1 Mar 2018 In : Journal of Steroid Biochemistry and Molecular Biology. 177, p. 59-69 11 p.

Research output: Contribution to journalArticle

Hydroxylation
Cholecalciferol
Vitamin D
Membranes
Mixed Function Oxygenases
2017
1 Citations

1 alpha,20S-Dihydroxyvitamin D-3 Interacts with Vitamin D Receptor: Crystal Structure and Route of Chemical Synthesis

Lin, Z., Chen, H., Belorusova, A. Y., Bollinger, J. C., Tang, E. K. Y., Janjetovic, Z., Kim, T-K., Wu, Z., Miller, D. D., Slominski, A. T., Postlethwaite, A. E., Tuckey, R. C., Rochel, N. & Li, W. 31 Aug 2017 In : Scientific Reports. 7, 10 p., 10193

Research output: Contribution to journalArticle

Open Access
1 Citations

Active Site Structures of CYP11A1 in the Presence of Its Physiological Substrates and Alterations upon Binding of Adrenodoxin

Zhu, Q., Mak, P. J., Tuckey, R. C. & Kincaid, J. R. 31 Oct 2017 In : Biochemistry. 56, 43, p. 5786-5797 12 p.

Research output: Contribution to journalArticle

Adrenodoxin
Cholesterol Side-Chain Cleavage Enzyme
Carbon Monoxide
Catalytic Domain
Substrates
2 Citations

Characterization of a new pathway that activates lumisterol in vivo to biologically active hydroxylumisterols

Slominski, A. T., Kim, T-K., Hobrath, J. V., Janjetovic, Z., Oak, A. S. W., Postlethwaite, A., Lin, Z., Li, W., Takeda, Y., Jetten, A. M. & Tuckey, R. C. 12 Sep 2017 In : Scientific Reports. 7, 1, 17 p., 11434

Research output: Contribution to journalArticle

Open Access
3 Citations

Cutaneous Glucocorticoidogenesis and Cortisol Signaling Are Defective in Psoriasis

Slominski, A. T., Brożyna, A. A. & Tuckey, R. C. 1 Aug 2017 In : Journal of Investigative Dermatology. 137, 8, p. 1609-1611 3 p.

Research output: Contribution to journalComment/debate

Psoriasis
Glucocorticoids
Hydrocortisone
Pro-Opiomelanocortin
Skin
13 Citations

Endogenously produced nonclassical vitamin D hydroxy-metabolites act as “biased” agonists on VDR and inverse agonists on RORα and RORγ

Slominski, A. T., Kim, T. K., Hobrath, J. V., Oak, A. S. W., Tang, E. K. Y., Tieu, E. W., Li, W., Tuckey, R. C. & Jetten, A. M. 1 Oct 2017 In : Journal of Steroid Biochemistry and Molecular Biology. 173, p. 42-56 15 p.

Research output: Contribution to journalArticle

Metabolites
Vitamin D
Secosteroids
Molecular modeling
Ligands
4 Citations

Noncalcemic 20-hydroxyvitamin D3 inhibits human melanoma growth in in vitro and in vivo models

Skobowiat, C., Oak, A. S. W., Kim, T. K., Yang, C. H., Pfeffer, L. M., Tuckey, R. C. & Slominski, A. T. 2017 In : Oncotarget. 8, 6, p. 9823-9834 12 p.

Research output: Contribution to journalArticle

Open Access
Hydroxycholecalciferols
Melanoma
Growth
Agar
Secosteroids
11 Citations

Vitamin D signaling and melanoma: Role of Vitamin D and its receptors in melanoma progression and management

Slominski, A. T., Brozyna, A. A., Zmijewski, M. A., Józwicki, W., Jetten, A. M., Mason, R. S., Tuckey, R. C. & Elmets, C. A. 1 Jun 2017 In : Laboratory Investigation. 97, 6, p. 706-724 19 p.

Research output: Contribution to journalReview article

Calcitriol Receptors
Vitamin D
Melanoma
25-Hydroxyvitamin D3 1-alpha-Hydroxylase
Mixed Function Oxygenases
2016
3 Citations

Bioactive forms of vitamin D selectively stimulate the skin analog of the hypothalamus-pituitary-adrenal axis in human epidermal keratinocytes

Wierzbicka, J. M., Żmijewski, M. A., Piotrowska, A., Nedoszytko, B., Lange, M., Tuckey, R. & Slominski, A. T. 2016 In : Molecular and Cellular Endocrinology. 437, p. 312-322

Research output: Contribution to journalArticle

Cholecalciferol
Keratinocytes
Vitamin D
Hypothalamus
Skin
19 Citations

Classical and non-classical metabolic transformation of vitamin D in dermal fibroblasts

Slominski, A. T., Kim, T. K., Li, W. & Tuckey, R. 2016 In : Experimental Dermatology. 25, 3, p. 231-232

Research output: Contribution to journalArticle

Design, Synthesis and Biological Activities of Novel Gemini 20S-Hydroxyvitamin D-3 Analogs

Lin, Z., Marepally, S. R., Kim, T., Janjetovic, Z., Oak, A. S. W., Postlethwaite, A. E., Myers, L. K., Tuckey, R. C., Slominski, A. T., Miller, D. D. & Li, W. 2016 In : Anticancer Research. 36, 3, p. 877-886 10 p.

Research output: Contribution to journalArticle

Calcitriol Receptors
Hydroxycholecalciferols
Cholesterol Side-Chain Cleavage Enzyme
Anti-Inflammatory Agents
Transient Receptor Potential Channels
7 Citations

Hydroxylation of 20-hydroxyvitamin D3 by human CYP3A4

Cheng, C., Slominski, A. T. & Tuckey, R. 2016 In : Journal of Steroid Biochemistry and Molecular Biology. 159, p. 131-141

Research output: Contribution to journalArticle

Cytochrome P-450 CYP3A
Hydroxylation
Liver
Cell proliferation
Liver Microsomes
2 Citations

Membrane-Mediated Protein–Protein Interactions of Cholesterol Side-Chain Cleavage Cytochrome P450 with its Associated Electron Transport Proteins

Kubeil, C., Yeung, J. C. I., Tuckey, R. C., Rodgers, R. J. & Martin, L. L. Sep 2016 In : ChemPlusChem. 81, 9, p. 995-1002

Research output: Contribution to journalArticle

Cytochrome P-450 Enzyme System
Adrenodoxin
Carrier Proteins
Cholesterol
Membranes
6 Citations

Synthesis and Biological Evaluation of Vitamin D3 Metabolite 20S,23S-Dihydroxyvitamin D3 and Its 23R Epimer

Lin, Z., Marepally, S. R., Ma, D., Kim, T. K., Oak, A. S., Myers, L. K., Tuckey, R., Slominski, A. T., Miller, D. D. & Li, W. 2016 In : Journal of Medicinal Chemistry. 59, 10, p. 5102-5108

Research output: Contribution to journalArticle

Cholecalciferol
25-Hydroxyvitamin D3 1-alpha-Hydroxylase
Calcitriol Receptors
Esters
Anti-Inflammatory Agents
3 Citations

Vitamin D derivatives enhance cytotoxic effects of H2O2 or cisplatin on human keratinocytes

Piotrowska, A., Wierzbicka, J., Ślebioda, T., Woźniak, M., Tuckey, R., Slominski, A. T. & Zmijewski, M. A. 2016 In : Steroids. 110, p. 49-61

Research output: Contribution to journalArticle

Keratinocytes
Vitamin D
Cisplatin
Derivatives
Membrane Potentials
2015
13 Citations

Antitumor effects of vitamin D analogs on hamster and mouse melanoma cell lines in relation to melanin pigmentation

Wasiewicz, T., Szyszka, P., Cichorek, M., Janjetovic, Z., Tuckey, R., Słomiński, A. T. & Zmijewski, M. A. 2015 In : International Journal of Molecular Sciences. 16, 4, p. 6645-6667

Research output: Contribution to journalArticle

Open Access
calciferol
Melanin
melanin
hamsters
Vitamins
5 Citations

A proposed molecular mechanism of high-dose vitamin D3 supplementation in prevention and treatment of preeclampsia

Zabul, P., Wozniak, M., Slominski, A. T., Preis, K., Gorska, M., Korozan, M., Wieruszewski, J., Zmijewski, M. A., Zabul, E., Tuckey, R., Kuban-Jankowska, A., Mickiewicz, W. & Knap, N. 2015 In : International Journal of Molecular Sciences. 16, 6, p. 13043-13064

Research output: Contribution to journalArticle

Open Access
Cholesterol Side-Chain Cleavage Enzyme
vitamins
Cholecalciferol
Vitamins
cytochromes

Biochemistry in Western Australia

Newsholme, P., Hartmann, P., Tuckey, R. & Bond, C. 2015 In : Biochemist. 37, 6, p. 32-34

Research output: Contribution to journalArticle

11 Citations

Chemical Synthesis and Biological Activities of 20S,24S/R-Dihydroxyvitamin D3 Epimers and Their 1α-Hydroxyl Derivatives

Lin, Z., Marepally, S. R., Ma, D., Myers, L. K., Postlethwaite, A. E., Tuckey, R., Cheng, C., Kim, T. K., Yue, J., Slominski, A. T., Miller, D. D. & Li, W. 2015 In : Journal of Medicinal Chemistry. 58, 19, p. 7881-7887

Research output: Contribution to journalArticle

Hydroxyl Radical
25-Hydroxyvitamin D3 1-alpha-Hydroxylase
Calcitriol Receptors
Cholecalciferol
Hydroxylation
14 Citations

CYP11A1 in skin: An alternative route to photoprotection by vitamin D compounds

Tongkao-On, W., Carter, S. E., Reeve, V. E., Dixon, K. M., Gordon-Thomson, C., Halliday, G. M., Tuckey, R. & Mason, R. S. Apr 2015 In : Journal of Steroid Biochemistry and Molecular Biology. 148, p. 72-78

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Vitamin D
Ultraviolet radiation
Skin
Radiation
50 Citations

Detection of novel CYP11A1-derived secosteroids in the human epidermis and serum and pig adrenal gland

Slominski, A. T., Kim, T. K., Li, W., Postlethwaite, A., Tieu, E., Tang, E. & Tuckey, R. 2015 In : Scientific Reports. 5, p. 14875

Research output: Contribution to journalArticle

Open Access
Secosteroids
Cholesterol Side-Chain Cleavage Enzyme
Bioactivity
Cholecalciferol
Metabolism
11 Citations

Differential antitumor effects of vitamin D analogues on colorectal carcinoma in culture

Wierzbicka, J. M., Binek, A., Ahrends, T., Nowac Ka, J. D., Szydłowska, A., Wąsiewic Z, T., Wierzbicki, P. M., Sądej, R., Tuckey, R., Slominski, A. T., Chybicki, J., Adrych, K., Kmieć, Z. & Zmijewski, M. A. 2015 In : International Journal of Oncology. 47, 3, p. 1084-1096

Research output: Contribution to journalArticle

Cholecalciferol
Vitamin D
Colorectal Neoplasms
Cell Line
Avitaminosis
10 Citations

Metabolism of 20-hydroxyvitamin D3 and 20,23-dihydroxyvitamin D3 by rat and human CYP24A1

Tieu, E., Li, W., Chen, J., Kim, T., Ma, D., Słomiński, A. T. & Tuckey, R. 2015 In : Journal of Steroid Biochemistry and Molecular Biology. 149, p. 153-165

Research output: Contribution to journalArticle

Metabolism
Rats
Hydroxylation
Metabolites
Protein Isoforms
65 Citations

Novel activities of CYP11A1 and their potential physiological significance

Slominski, A. T., Li, W., Kim, T-K., Semak, I., Wang, J., Zjawiony, J. K. & Tuckey, R. 2015 In : Journal of Steroid Biochemistry and Molecular Biology. 151, p. 25-37

Research output: Contribution to journalArticle

25 Citations

Novel non-calcemic secosteroids that are produced by human epidermal keratinocytes protect against solar radiation

Slominski, A. T., Janjetovic, Z., Kim, T. K., Wasilewski, P., Rosas, S., Hanna, S., Sayre, R. M., Dowdy, J. C., Li, W. & Tuckey, R. 2015 In : Journal of Steroid Biochemistry and Molecular Biology. 148, p. 52-63

Research output: Contribution to journalArticle

Secosteroids
Solar radiation
Keratinocytes
Cholesterol Side-Chain Cleavage Enzyme
Radiation
47 Citations

On the role of skin in the regulation of local and systemic steroidogenic activities

Slominski, A. T., Manna, P. R. & Tuckey, R. 2015 In : Steroids. 103, p. 72-88

Research output: Contribution to journalArticle

Skin
Cholesterol Side-Chain Cleavage Enzyme
Vitamin D
Pregnenolone
Tissue
2014
34 Citations

Cutaneous glucocorticosteroidogenesis: Securing local homeostasis and the skin integrity

Słomiński, A. T., Manna, P. R. & Tuckey, R. 2014 In : Experimental Dermatology. 23, 6, p. 369-374

Research output: Contribution to journalArticle

Skin
Homeostasis
Steroids
Ultraviolet Rays
Cyclic AMP-Dependent Protein Kinases
14 Citations

CYP24A1 Expression Inversely Correlates with Melanoma Progression: Clinic-Pathological Studies

Brozyna, A. A., Jochymski, C., Janjetovic, Z., Józwicki, W., Tuckey, R. & Slominski, A. T. 2014 In : International Journal of Molecular Sciences (Online). 15, p. 19000-19017

Research output: Contribution to journalArticle

Open Access
44 Citations

Cytochromes P450 and skin cancer: Role of local endocrine pathways

Słomiński, A. T., Zmijewski, M. A., Semak, I. V., Zbytek, B., Pisarchik, A. V., Li, W., Zjawiony, J. K. & Tuckey, R. 2014 In : Anti-Cancer Agents in Medicinal Chemistry. 14, 1, p. 77-96

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Skin Neoplasms
Cytochrome P-450 Enzyme System
Skin
Hydroxylation
34 Citations

In vivo production of novel vitamin D2 hydroxy-derivatives by human placentas, epidermal keratinocytes, Caco-2 colon cells and the adrenal gland

Słomiński, A. T., Kim, T., Shehabi, H. Z., Tang, E. K. Y., Benson, H. A. E., Semak, I. V., Lin, Z., Yates, C. R., Wang, J., Li., W. & Tuckey, R. 2014 In : Molecular and Cellular Endocrinology. 383, 1-2, p. 181-192

Research output: Contribution to journalArticle

Ergocalciferols
25-Hydroxyvitamin D3 1-alpha-Hydroxylase
Caco-2 Cells
Cholesterol Side-Chain Cleavage Enzyme
Adrenal Glands
16 Citations

Kinetic analysis of human CYP24A1 metabolism of vitamin D via the C24-oxidation pathway

Tieu, E. W., Tang, E. K. Y. & Tuckey, R. 2014 In : FEBS Journal. 281, 14, p. 3280-3296

Research output: Contribution to journalArticle

Enzyme kinetics
Metabolism
Vitamin D
Phospholipids
Oxidation
8 Citations

Lumisterol is metabolized by CYP11A1: Discovery of a new pathway

Tuckey, R., Słomiński, A. T., Cheng, C., Chen, J., Kim, T., Xiao, M. & Li, W. 2014 In : International Journal of Biochemistry and Cell Biology. 55, p. 24-34

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Ergosterol
Bioactivity
Metabolism
Cholecalciferol
8 Citations

Metabolism of 20-hydroxyvitamin D3 by mouse liver microsomes

Cheng, C., Słomiński, A. T. & Tuckey, R. 2014 In : Journal of Steroid Biochemistry and Molecular Biology. 144, PART B, p. 286-293

Research output: Contribution to journalArticle

Liver Microsomes
Metabolism
Liver
Metabolites
Cholesterol Side-Chain Cleavage Enzyme
23 Citations

Novel vitamin D analogs as potential therapeutics: Metabolism, toxicity profiling, and antiproliferative activity

Chen, J., Wang, J., Kim, T., Tieu, E., Tang, E., Lin, Z., Kovacic, D., Miller, D. D., Postlethwaite, A. E., Tuckey, R., Słomiński, A. T. & Li., W. 2014 In : Anticancer Research. 34, 5, p. 2153-2164

Research output: Contribution to journalArticle

Vitamin D
Calcitriol Receptors
Liver Microsomes
Enzymes
Keratinocytes
63 Citations

RORo: And ROR 7 are expressed in human skin and serve as receptors for endogenously produced noncalcemic 20-hydroxy- and 20,23-dihydroxyvitamin D

Słomiński, A. T., Kim, T., Takeda, Y., Janjetovic, Z., Brozyna, A. A., Skobowiat, C., Wang, J., Postlethwaite, A. E., Li, W., Tuckey, R. & Jetten, A. M. 2014 In : FASEB Journal. 28, 7, p. 2775-2789

Research output: Contribution to journalArticle

Dihydroxycholecalciferols
Skin
Ligands
Chemical activation
Jurkat Cells
62 Citations

The role of CYP11A1 in the production of vitamin D metabolites and their role in the regulation of epidermal functions

Slominski, A. T., Kim, T-K., Li, W., Yi, A-K., Postlethwaite, A. & Tuckey, R. 2014 In : Journal of Steroid Biochemistry and Molecular Biology. N/A, p. 12pp

Research output: Contribution to journalArticle

2013
40 Citations

20S-hydroxyvitamin D3, noncalcemic product of CYP11A1 action on vitamin D3, exhibits potent antifibrogenic activity in vivo

Słomiński, A., Janjetovic, Z., Tuckey, R., Nguyen, M., Bhattacharya, K. G., Wang, J., Li, W., Jiao, Y., Gu, W., Brown, M. & Postlethwaite, A. E. 2013 In : Journal of Clinical Endocrinology and Metabolism. 98, 2, p. E298-E303

Research output: Contribution to journalArticle

Cholesterol Side-Chain Cleavage Enzyme
Cholecalciferol
Fibroblasts
Hydroxycholecalciferols
Collagen
19 Citations

Hydroxylation of CYP11A1-derived products of vitamin D3 metabolism by human and Mouse CYP27B1

Tang, E. K. Y., Chen, J., Janjetovic, Z., Tieu, E., Słomiński, A. T., Li, W. & Tuckey, R. 2013 In : Drug Metabolism and Disposition. 41, 5, p. 1112-1124

Research output: Contribution to journalArticle

25-Hydroxyvitamin D3 1-alpha-Hydroxylase
Cholesterol Side-Chain Cleavage Enzyme
Cholecalciferol
Hydroxylation
Secosteroids

Novel metabolism of vitamin D in human epidermal keratinocytes and epithelial colon cells

Kim, T., Lin, Z., Tuckey, R., Tang, E., Li, W. & Slominski, A. T. 2013 In : Journal of Investigative Dermatology. 133, p. S116-S116

Research output: Contribution to journalReview article

31 Citations

Novel vitamin D photoproducts and their precursors in the skin

Słomiński, A. T., Kim, T., Zmijewski, M. A., Janjetovic, Z., Li, W., Chen, J., Kusniatsova, E. I., Semak, I. V., Postlethwaite, A. E., Miller, D. D., Zjawiony, J. K. & Tuckey, R. 2013 In : Dermato-Endocrinology. 5, 1, p. 7-19

Research output: Contribution to journalReview article

Cholesterol Side-Chain Cleavage Enzyme
Secosteroids
Vitamin D
Ergocalciferols
Cholecalciferol
29 Citations
141 Citations

Steroidogenesis in the skin: Implications for local immune functions

Słomiński, A. T., Zbytek, B., Nikolakis, G., Manna, P. R., Skobowiat, C., Zmijewski, M. A., Li, W., Janjetovic, Z., Postlethwaite, A. E., Zouboulis, C. C. Z. & Tuckey, R. 2013 In : Journal of Steroid Biochemistry and Molecular Biology. 137, p. 107-123

Research output: Contribution to journalArticle

Skin
Glucocorticoids
Androgens
Estrogens
Steroids
2012
36 Citations

20-Hydroxyvitamin D3 inhibits proliferation of cancer cells with high efficacy while being non-toxic

Wang, J., Slominski, A., Tuckey, R., Janjetovic, Z., Kulkarni, A., Chen, J., Postlethwaite, A. E., Miller, D. & Li, W. 2012 In : Anticancer Research. 32, p. 739-746

Research output: Contribution to journalArticle

30 Citations

Acute Erythemal Ultraviolet Radiation Causes Systemic Immunosuppression in the Absence of Increased 25-Hydroxyvitamin D3 Levels in Male Mice

Gorman, S., Scott, N. M., Tan, D. H. W., Weeden, C. E., Tuckey, R., Bisley, J. L., Grimbaldeston, M. A. & Hart, P. 26 Sep 2012 In : PLoS One. 7, 9, p. e46006.1-12

Research output: Contribution to journalArticle

Open Access
25-hydroxycholecalciferol
Calcifediol
cholecalciferol
Cholecalciferol
immunosuppression
32 Citations

Correlation between secosteroid-induced vitamin D receptor activity in melanoma cells and computer-modeled receptor binding strength

Kim, T-K., Wang, J., Janjetovic, Z., Chen, J., Tuckey, R., Nguyen, M., Tang, E. K. Y., Miller, D., Li, W. & Slominski, A. T. 2012 In : Molecular and Cellular Endocrinology. 361, p. 143-152

Research output: Contribution to journalArticle

39 Citations

Cytochrome P450scc-dependent metabolism of 7-dehydrocholesterol in placenta and epidermal keratinocytes

Slominski, A. T., Kim, T-K., Chen, J., Nguyen, M., Li, W., Yates, C. R., Sweatman, T., Janjetovic, Z. & Tuckey, R. 2012 In : International Journal of Biochemistry & Cell Biology. 44, p. 2003– 2018

Research output: Contribution to journalArticle

20 Citations

Design, synthesis, and biological action of 20 R -hydroxyvitamin D3

Lu, Y., Chen, J., Janjetovic, Z., Michaels, P., Tang, E. K. Y., Wang, J., Tuckey, R., Slominski, A. T., Li, W. & Miller, D. D. 2012 In : Journal of Medicinal Chemistry. 55, p. 3573−3577

Research output: Contribution to journalArticle

7 Citations

Expression of human CYP27B1 in Escherichia coli and characterization in phospholipid vesicles

Tang, E. K. Y., Tieu, E. & Tuckey, R. 2012 In : FEBS Journal. 279, p. 3749

Research output: Contribution to journalArticle

16 Citations

Human cytochrome P450scc (CYP11A1) Catalyzes Epoxide Formation with Ergosterol

Tuckey, R., Nguyen, M., Chen, J., Slominski, A. T., Baldisseri, D. M., Tieu, E., Zjawiony, J. K. & Li, W. 2012 In : Drug Metabolism and Disposition. 40, 3, p. 436–444

Research output: Contribution to journalArticle