Research Output per year
My work at UWA has required me to apply my extended knowledge in the fields of metabolomics and fetal programming research. My areas of expertise are GC/MS, LC/MS, histology, microscopy and stereology, as well as animal handling, gene expression analysis and tissue culture. This includes preparation of specialized equipment, method development, training and supervision of users, optimizing outcomes of experiments and ensuring precise analysis of results. I have gained a broad level of experience from a number of facilities, both in Australia and in Germany. This has seen me develop strong skills in all aspects of laboratory and research operations.
Roles and responsibilities
Senior Technical Officer
UWA, Centre for Microscopy,Characterisationand Analysis (CMCA), Metabolomics Australia
At Metabolomics Australia I have gained and applied a broad range of new skills and expertise in the field of metabolomics.
Independently, I have provided a high level of technical support to laboratories, performed demonstrations and I have maintained and delivered metabolomics services to the biosciences community including a range of different analytical areas.
My current position gives me the opportunity to show expertise in identifying and solving problems in the fields of method development, sample preparation, troubleshooting and general operation and maintenance of gas chromatography (GC) and mass spectrometry (MS) analysing polar metabolites (TMS and TBS), fatty acid methyl esters, short chain fatty acids, terpenoids and others in various samples as well as SPME analysis for GC/ MS analysis in various sample types.
In addition, I acquired and applied my skills in liquid chromatography including Vitamin D, Steroid and Curcumin metabolite analysis (all original new methods).
I am very confident in handling a range of different software and databases required for GC MS and LC MS analysis such as ChemStation, MassHunter, AMDIS, NIST and GOLM. One of my many tasks is the development of new SOPs according to CMCA guidelines. This includes risk assessment analysis according to Occupational Health and Safety guidelines as well as the operation of the software ChemAlert. In addition, I am responsible for training, certification and supervision of clients and faculty for specific sample preparations, method development and machine operations on various machines. Currently, I am undergoing extended training in the operation and maintenance of GC TOF, LC TOF and the Thermo Orbitrap machines with expected completion of mid 2017.
Also, I have performed and delivered highly precise sample preparation including extensive quality control consistent with NATA guidelines ISO17025.
Graduate Reseach Assistant
University of Western Australia, School of Anatomy and Human Biology, Prof B.J. Waddell
Under the supervision of Prof B.J. Waddell I worked on various research projects in the field of Fetal Programming. In this position I lead a major animal study to identify the effects of Dexamethasone during pregnancy on the offspring and assisted in other research projects in collaboration with my supervisors.
The animal study included the dealing and handling with animals such as mating and smearing of rats and mice, tail cuff blood pressure measurements on rats, bone mineral density and body fat composition measurements, administration of substances via multiple routes as well as surgical procedures and dissections of various tissues in rodents.
My extended laboratory skills reached from managing the Stereological Investigator and microscopy equipment to support quantitative analysis of animal and human tissues including the training of students and staff. My histology and Immunohistochemistry skills were an excellent core foundation to support the development of new the techniques for morphometric analysis.
Furthermore, procedures such as genotyping (PCR), protein and RNA extractions, Western blots and tissue culture were part of my day to day tasks.
Prior to commencing with Professor Waddell and Dr Mark, I was actively engaged in a number of research fields and laboratories, both in Australia and in Germany. This has enabled me to develop a strong skillset in research techniques.
Developmental programming of adult adrenal structure and steroidogenesis: effects of fetal glucocorticoid excess and postnatal dietary omega-3 fatty acidsWaddell, B., Bollen, M., Wyrwoll, C. S., Mori, T. & Mark, P., 2010, In : Journal of Endocrinology. 205, p. 171-178
Research output: Contribution to journal › Article