• The University of Western Australia (M519), 35 Stirling Highway,

    6009 Perth

    Australia

  • 604 Citations
  • 13 h-Index
20082020

Research output per year

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Research Output

2020

Characterization of planar phosphorescence based oxygen sensors on a TCSPC-PLIM macro-imager

Sen, R., Zhdanov, A. V., Hirvonen, L. M., Svihra, P., Andersson-Engels, S., Nomerotski, A. & Papkovsky, D. B., 15 Oct 2020, In : Sensors and Actuators, B: Chemical. 321, 128459.

Research output: Contribution to journalArticle

Open Access

Combined AFM and super-resolution localisation microscopy: Investigating the structure and dynamics of podosomes

Hirvonen, L. M., Marsh, R. J., Jones, G. E. & Cox, S., Sep 2020, In : European Journal of Cell Biology. 99, 7, 151106.

Research output: Contribution to journalArticle

Open Access

Fast Timing Techniques in FLIM Applications

Hirvonen, L. M. & Suhling, K., 13 May 2020, In : Frontiers in Physics. 8, 161.

Research output: Contribution to journalReview article

Open Access

New luminescence lifetime macro-imager based on a Tpx3Cam optical camera

Sen, R., Hirvonen, L. M., Zhdanov, A., Svihra, P., Andersson-Engels, S., Nomerotski, A. & Papkovsky, D., 1 Jan 2020, In : Biomedical Optics Express. 11, 1, p. 77-88 12 p.

Research output: Contribution to journalArticle

5 Citations (Scopus)
2019

Fluorescence lifetime imaging for viscosity and diffusion measurements

Suhling, K., Teijeiro-Gonzalez, Y., Steinmark, I. E., James, A. L., Economou, A. M., Yahioglu, G., Le Marois, A., Hirvonen, L. M., Nedbal, J., Levitt, J. A., Chung, P. H., Dreiss, C. A., Beavil, A. J., Beavil, R. L., Ortiz-Zapater, E., Lorenz, C. D., Parsons, M., Crnjar, A., Cornell, B. & Molteni, C., 1 Jan 2019, Multiphoton Microscopy in the Biomedical Sciences XIX. Periasamy, A., So, P. T. C. & Konig, K. (eds.). USA: SPIE-INT SOC OPTICAL ENGINEERING, Vol. 10882. 1088210. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; vol. 10882).

Research output: Chapter in Book/Conference paperConference paper

Fluorescence Recovery After Photobleaching (FRAP) with simultaneous Fluorescence Lifetime and time-resolved Fluorescence Anisotropy Imaging (FLIM & tr-FAIM)

Teijeiro-Gonzalez, Y., Le Marois, A., Economou, A. M., Hirvonen, L. M., Levitt, J. A., Beavil, A. J., Beavil, R. L., Crnjar, A., Molteni, C., Ortiz-Zapater, E., Parsons, M. & Suhling, K., 21 Feb 2019, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI. Wilson, T. & Brown, T. G. (eds.). USA: SPIE-INT SOC OPTICAL ENGINEERING, Vol. 10883. 108830A. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; vol. 10883).

Research output: Chapter in Book/Conference paperConference paper

1 Citation (Scopus)

Lightsheet fluorescence lifetime imaging microscopy (FLIM) with wide-field time-correlated single photon counting (TCSPC)

Hirvonen, L. M., Nedbal, J., Almutairi, N., Phillips, T. A., Becker, W., Conneely, T., Milnes, J., Cox, S., Stürzenbaum, S. & Suhling, K., 29 Oct 2019, In : Journal of Biophotonics. e201960099.

Research output: Contribution to journalArticle

Open Access
2 Citations (Scopus)

PAK4 Kinase Activity Plays a Crucial Role in the Podosome Ring of Myeloid Cells

Foxall, E., Staszowska, A., Hirvonen, L. M., Georgouli, M., Ciccioli, M., Rimmer, A., Williams, L., Calle, Y., Moreno, V. S., Cox, S., Jones, G. E. & Wells, C. M., 10 Dec 2019, In : Cell Reports. 29, 11, p. 3385-3393.e6

Research output: Contribution to journalArticle

Open Access
1 Citation (Scopus)

Singlet–Triplet Transition Rate Enhancement inside Hyperbolic Metamaterials

Roth, D. J., Ginzburg, P., Hirvonen, L. M., Levitt, J. A., Nasir, M. E., Suhling, K., Richards, D., Podolskiy, V. A. & Zayats, A. V., 1 Jan 2019, In : Laser and Photonics Reviews. 1900101.

Research output: Contribution to journalArticle

4 Citations (Scopus)

Wide-field time-correlated single photon counting-based fluorescence lifetime imaging microscopy

Suhling, K., Hirvonen, L. M., Becker, W., Smietana, S., Netz, H., Milnes, J., Conneely, T., Marois, A. L., Jagutzki, O., Festy, F., Petrášek, Z. & Beeby, A., 21 Oct 2019, In : Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. 942, 162365.

Research output: Contribution to journalReview article

Open Access
2 Citations (Scopus)
2018

Artifact-free high-density localization microscopy analysis

Marsh, R. J., Pfisterer, K., Bennett, P., Hirvonen, L. M., Gautel, M., Jones, G. E. & Cox, S., 1 Sep 2018, In : Nature Methods. 15, 9, p. 689-692 4 p.

Research output: Contribution to journalArticle

27 Citations (Scopus)

Cardiomyocytes Sense Matrix Rigidity through a Combination of Muscle and Non-muscle Myosin Contractions

Pandey, P., Hawkes, W., Hu, J., Megone, W. V., Gautrot, J., Anilkumar, N., Zhang, M., Hirvonen, L., Cox, S., Ehler, E., Hone, J., Sheetz, M. & Iskratsch, T., 5 Feb 2018, In : Developmental Cell. 44, 3, p. 326-336.e3

Research output: Contribution to journalArticle

Open Access
30 Citations (Scopus)
Open Access
2 Citations (Scopus)

The Rényi divergence enables accurate and precise cluster analysis for localization microscopy

Staszowska, A. D., Fox-Roberts, P., Hirvonen, L. M., Peddie, C. J., Collinson, L. M., Jones, G. E. & Cox, S., 1 Jan 2018, In : Bioinformatics. 34, 23, p. 4102-4111 10 p.

Research output: Contribution to journalArticle

Open Access
1 Citation (Scopus)
2017

Fluorescence lifetime imaging

Suhling, K., Hirvonen, L. M., Levitt, J. A., Chung, P. H., Tregidgo, C., Rusakov, D. A., Zheng, K., Ameer-Beg, S., Poland, S. P., Coelho, S., Henderson, R. & Krstajic, N., 29 Mar 2017, Handbook of Photonics for Biomedical Engineering. Springer, Dordrecht, Netherlands, p. 353-405 53 p.

Research output: Chapter in Book/Conference paperChapter

3 Citations (Scopus)

Photon counting phosphorescence lifetime imaging with TimepixCam

Hirvonen, L. M., Fisher-Levine, M., Suhling, K. & Nomerotski, A., 1 Jan 2017, In : Review of Scientific Instruments. 88, 1, 013104.

Research output: Contribution to journalArticle

Open Access
13 Citations (Scopus)

Spontaneous emission in non-local materials

Ginzburg, P., Roth, D. J., Nasir, M. E., Segovia, P., Krasavin, A. V., Levitt, J., Hirvonen, L. M., Wells, B., Suhling, K., Richards, D., Podolskiy, V. A. & Zayats, A. V., 2 Jun 2017, In : Light: Science and Applications. 6, 6, e16273.

Research output: Contribution to journalArticle

Open Access
45 Citations (Scopus)

Wide-field TCSPC: Methods and applications

Hirvonen, L. M. & Suhling, K., 1 Jan 2017, In : Measurement Science and Technology. 28, 1, 012003.

Research output: Contribution to journalReview article

Open Access
28 Citations (Scopus)
2016

A wide-field TCSPC FLIM system based on an MCP PMT with a delay-line anode

Becker, W., Hirvonen, L. M., Milnes, J., Conneely, T., Jagutzki, O., Netz, H., Smietana, S. & Suhling, K., 1 Sep 2016, In : Review of Scientific Instruments. 87, 9, 093710.

Research output: Contribution to journalArticle

Open Access
14 Citations (Scopus)

Hydrodynamic Radii of Ranibizumab, Aflibercept and Bevacizumab Measured by Time-Resolved Phosphorescence Anisotropy

Hirvonen, L. M., Fruhwirth, G. O., Srikantha, N., Barber, M. J., Neffendorf, J. E., Suhling, K. & Jackson, T. L., 1 Aug 2016, In : Pharmaceutical Research. 33, 8, p. 2025-2032 8 p.

Research output: Contribution to journalArticle

Open Access
19 Citations (Scopus)
Open Access
7 Citations (Scopus)

Photon counting imaging with an electron-bombarded pixel image sensor

Hirvonen, L. M. & Suhling, K., 1 May 2016, In : Sensors (Switzerland). 16, 5, 617.

Research output: Contribution to journalReview article

Open Access
6 Citations (Scopus)

Picosecond wide-field time-correlated single photon counting fluorescence microscopy with a delay line anode detector

Hirvonen, L. M., Becker, W., Milnes, J., Conneely, T., Smietana, S., Le Marois, A., Jagutzki, O. & Suhling, K., 15 Aug 2016, In : Applied Physics Letters. 109, 7, 071101.

Research output: Contribution to journalArticle

Open Access
14 Citations (Scopus)

Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy

Suhling, K., Hirvonen, L. M., Becker, W., Smietana, S., Netz, H., Milnes, J., Conneely, T., Le Marois, A. & Jagutzki, O., 5 May 2016, Advanced Photon Counting Techniques X. Campbell, J. C. & Itzler, M. A. (eds.). USA: SPIE-INT SOC OPTICAL ENGINEERING, 98580J. (Proceedings of SPIE - The International Society for Optical Engineering; vol. 9858).

Research output: Chapter in Book/Conference paperConference paper

4 Citations (Scopus)
2015

Erratum to: Fluorescence lifetime imaging (FLIM): Basic concepts and recent applications (Springer series in chemical physics, 111, 10.1007/978-3-319-14929-5_3)

Suhling, K., Hirvonen, L. M., Levitt, J. A., Chung, P. H., Tregido, C., Marois, A. L., Rusakov, D. A., Zheng, K., Ameer-Beg, S., Poland, S. & Coelho, S., 1 Jan 2015, In : Springer Series in Chemical Physics. 111

Research output: Contribution to journalComment/debate

Fluorescence lifetime imaging (Flim): Basic concepts and recent applications

Suhling, K., Hirvonen, L. M., Levitt, J. A., Chung, P. H., Tregido, C., Marois, A. L., Rusakov, D. A., Zheng, K., Ameer-Beg, S., Poland, S., Coelho, S. & Dimble, R., 1 Jan 2015, In : Springer Series in Chemical Physics. 111, p. 119-188 70 p.

Research output: Contribution to journalArticle

7 Citations (Scopus)

Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments

Suhling, K., Hirvonen, L. M., Levitt, J. A., Chung, P. H., Tregidgo, C., Le Marois, A., Rusakov, D. A., Zheng, K., Ameer-Beg, S., Poland, S., Coelho, S., Henderson, R. & Krstajic, N., 1 May 2015, In : Medical Photonics. 27, p. 3-40 38 p.

Research output: Contribution to journalReview article

95 Citations (Scopus)

Microsecond wide-field TCSPC microscopy based on an ultra-fast CMOS camera

Hirvonen, L. M., Petrášek, Z., Beeby, A. & Suhling, K., 1 Jan 2015, Multiphoton Microscopy in the Biomedical Sciences XV. Konig, K., So, P. T. C. & Periasamy, A. (eds.). USA: SPIE-INT SOC OPTICAL ENGINEERING, Vol. 9329. 932939. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; vol. 9329).

Research output: Chapter in Book/Conference paperConference paper

4 Citations (Scopus)

Multiphoton luminescence imaging of chemically functionalized multi-walled carbon nanotubes in cells and solid tumors

Rubio, N., Hirvonen, L. M., Chong, E. Z., Wang, J. T. W., Bourgognon, M., Kafa, H., Hassan, H. A. F. M., Al-Jamal, W. T., Mccarthy, D., Hogstrand, C., Festy, F. & Al-Jamal, K. T., 7 Jun 2015, In : Chemical Communications. 51, 45, p. 9366-9369 4 p.

Research output: Contribution to journalArticle

11 Citations (Scopus)
10 Citations (Scopus)

Single-molecule localization software applied to photon counting imaging

Hirvonen, L. M., Kilfeather, T. & Suhling, K., 1 Jun 2015, In : Applied Optics. 54, 16, p. 5074-5082 9 p.

Research output: Contribution to journalArticle

3 Citations (Scopus)

Sub-μs time resolution in wide-field time-correlated single photon counting microscopy obtained from the photon event phosphor decay

Hirvonen, L. M., Petrášek, Z., Beeby, A. & Suhling, K., 10 Feb 2015, In : New Journal of Physics. 17, 023032.

Research output: Contribution to journalArticle

Open Access
17 Citations (Scopus)
8 Citations (Scopus)
2014

Multimodal optical characterisation of collagen photodegradation by femtosecond infrared laser ablation

Manickavasagam, A., Hirvonen, L. M., Melita, L. N., Chong, E. Z., Cook, R. J., Bozec, L. & Festy, F., 27 Oct 2014, In : Analyst. 139, 23, p. 6135-6143 9 p.

Research output: Contribution to journalArticle

Open Access
10 Citations (Scopus)

Photon counting imaging with an electron-bombarded CCD: Towards a parallel-processing photoelectronic time-to-amplitude converter

Hirvonen, L. M., Jiggins, S., Sergent, N., Zanda, G. & Suhling, K., 1 Dec 2014, In : Review of Scientific Instruments. 85, 12, 123102.

Research output: Contribution to journalArticle

13 Citations (Scopus)

Wide-field time-correlated single-photon counting (TCSPC) lifetime microscopy with microsecond time resolution

Hirvonen, L. M., Festy, F. & Suhling, K., 1 Oct 2014, In : Optics Letters. 39, 19, p. 5602-5605 4 p.

Research output: Contribution to journalArticle

42 Citations (Scopus)
2013
10 Citations (Scopus)
2012

Deep-UV confocal fluorescence imaging and super-resolution optical microscopy of biological samples

Smith, T. A., Hirvonen, L. M., Lincoln, C. N. & Hao, X., 1 Oct 2012, In : Journal of Innovative Optical Health Sciences. 5, 4, 1250025.

Research output: Contribution to journalArticle

7 Citations (Scopus)
2011

Imaging on the nanoscale: Super-resolution fluorescence microscopy

Hirvonen, L. M. & Smith, T. A., 24 Jan 2011, In : Australian Journal of Chemistry. 64, 1, p. 41-45 5 p.

Research output: Contribution to journalArticle

10 Citations (Scopus)

Structured illumination microscopy of living cells

Hirvonen, L. M. & Smith, T. A., 1 Dec 2011, Conference on Lasers and Electro-Optics/Pacific Rim, CLEOPR 2011. p. 766-768 3 p. (Optics InfoBase Conference Papers).

Research output: Chapter in Book/Conference paperConference paper

2010

Structured Illumination and Image Inversion Interferometry

Wicker, K., Walde, M., Oldewurtel, E. R., Hirvonen, L., Mandula, O., Sindbert, S. & Heintzmann, R., 1 Jan 2010, In : Biophysical Journal. 98, 3

Research output: Contribution to journalArticle

2009

Structured illumination microscopy of a living cell

Hirvonen, L. M., Wicker, K., Mandula, O. & Heintzmann, R., 1 Jul 2009, In : European Biophysics Journal. 38, 6, p. 807-812 6 p.

Research output: Contribution to journalArticle

112 Citations (Scopus)
2008

Structured illumination microscopy using photoswitchable fluorescent proteins

Hirvonen, L., Mandula, O., Wicker, K. & Heintzmann, R., 21 Apr 2008, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV. 68610L. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; vol. 6861).

Research output: Chapter in Book/Conference paperConference paper

17 Citations (Scopus)