Personal profile
Biography
I am an experimental physicist / biophysicist interested in all aspects of optical microscopy. I obtained my PhD in Physics in 2008 from King’s College London, UK, in developing the (at the time novel) Structured Illumination Microscopy (SIM) super-resolution technique. I then held several postdoctoral positions at the University of Melbourne, Australia, and King’s College London, UK, where I continued to develop novel super-resolution and time-resolved fluorescence microscopy methods, and gained extensive experience in a broad range of optical microscopy techniques (including multiphoton, TIRF, lightsheet and atomic force microscopy, various super-resolution methods and fluorescence lifetime imaging (FLIM)).
I joined the Centre for Microscopy, Characterisation and Analysis (CMCA) at UWA in 2019 as a lecturer and leader of the Optical Microscopy platform. I oversee all CMCA Optical Microscopy equipment and provide training and ongoing support to the users of the Optical Microscopy platform.
Research
I am interested in a large range of optical microscopy techniques, and their applications to many fields of science. While my background is in instrument development, I now work in a core facility where my main focus is to support the users of our (mainly commercially available) microscopes, and therefore my focus has shifted from technique development more towards applications.
I am specifically interested in specialised fluorescence microscopy methods, including super-resolution and lightsheet microscopy, and fluorescence lifetime imaging (FLIM). I am always happy to try to find novel imaging solutions to problems that require something beyond conventional imaging techniques.
CMCA incorporates many different microscopy and analysis platforms, which enables correlative and multimodal imaging within the same facility. Together with other platforms at CMCA, I am developing correlative and multimodal workflows that combine optical microscopy techniques with other imaging methods, including Electron Microscopy, μCT, and nanoSIMs.
Teaching overview
My primary teaching role is to provide training and support for the Optical Microscopy platform users, many of whom are HDR students. I have developed and maintain an online training program for new users of our microscopy equipment, as well as providing practical assistance and support with projects. I also run a microscopy basics and image processing courses that are open to all WA researchers.
Additionally, I contribute to undergraduate teaching units in which light microscopy plays a central role. I am currently teaching part of the SCIE5516 unit (Materials Characterisation for Biomedical Engineering and Synthetic Biology), and have developed confocal & image processing practicals for BMEG5001 unit (Advanced Topics in Biomedical Engineering).
Roles and responsibilities
Platform Leader - CMCA Optical Microscopy
WA State Rep - Light Microscopy Australia
WA State Rep - Australia and New Zealand Society for Cell and Developmental Biology (ANZSCDB)
Education/Academic qualification
Physics, PhD, King's College London
Jul 2005 → Dec 2008
Award Date: 1 Dec 2008
Physics with Computer Science, BSc, King's College London
Sept 2002 → Jun 2005
Award Date: 30 Jun 2005
Industry keywords
- Biotechnology
- Bioengineering
- Medicine and Pharma
- Agriculture and Food
- Oceans
- Mining and Resources
Research expertise keywords
- Optical Microscopy
- Fluorescence microscopy
- FLIM
- Super-resolution
- Photon counting
- Cell imaging
- Correlative microscopy
- Cell biology
- CLEM
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
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SDG 9 Industry, Innovation, and Infrastructure
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Collaborations and top research areas from the last five years
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Lightsheet fluorescence lifetime imaging microscopy (FLIM) with wide-field time-correlated single photon counting (TCSPC)
Hirvonen, L. M., Nedbal, J., Almutairi, N., Phillips, T. A., Becker, W., Conneely, T., Milnes, J., Cox, S., Stürzenbaum, S. & Suhling, K., 1 Feb 2020, In: Journal of Biophotonics. 13, 2, e201960099.Research output: Contribution to journal › Article › peer-review
Open Access41 Link opens in a new tab Citations (Scopus) -
Fast Timing Techniques in FLIM Applications
Hirvonen, L. M. & Suhling, K., 13 May 2020, In: Frontiers in Physics. 8, 161.Research output: Contribution to journal › Review article › peer-review
Open Access52 Link opens in a new tab Citations (Scopus) -
Artifact-free high-density localization microscopy analysis
Marsh, R. J., Pfisterer, K., Bennett, P., Hirvonen, L. M., Gautel, M., Jones, G. E. & Cox, S., 1 Sept 2018, In: Nature Methods. 15, 9, p. 689-692 4 p.Research output: Contribution to journal › Article › peer-review
81 Link opens in a new tab Citations (Scopus) -
Wide-field TCSPC: Methods and applications
Hirvonen, L. M. & Suhling, K., 1 Jan 2017, In: Measurement Science and Technology. 28, 1, 012003.Research output: Contribution to journal › Review article › peer-review
Open Access85 Link opens in a new tab Citations (Scopus) -
How did correlative atomic force microscopy and super-resolution microscopy evolve in the quest for unravelling enigmas in biology?
Miranda, A., Gómez-Varela, A. I., Stylianou, A., Hirvonen, L. M., Sánchez, H. & De Beule, P. A. A., 2021, In: Nanoscale. 13, 4, p. 2082-2099 18 p.Research output: Contribution to journal › Review article › peer-review
Open Access35 Link opens in a new tab Citations (Scopus)
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Developing a Model of Human Microvasculature: Development and Optimization of Confocal and Live Imaging
Bosio, E. (Investigator 01), Macdonald, S. (Investigator 02), Kenny, J. (Investigator 03), Hirvonen, L. (Investigator 04) & Juan Pardo, E. (Investigator 05)
Royal Perth Hospital Medical Research Foundation
13/03/25 → 13/03/26
Project: Research
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WA lightsheet microscopy facility for fast and gentle volumetric imaging
Hirvonen, L. (Investigator 01), Abbott, L. (Investigator 02), Lister, R. (Investigator 03), Rodger, J. (Investigator 04) & Juan Pardo, E. (Investigator 05)
ARC Australian Research Council
1/01/25 → 31/12/25
Project: Research
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Lionheart FX Automated High-Content Microscope for the QEII Biomedical Research Precinct
Pavlos, N. (Chief Investigator), Barnett, T. (CoPI), Blancafort, P. (CoPI), Chen, K. (CoPI), Chooi, H. (CoPI), Hamzah, J. (CoPI), Hirvonen, L. (CoPI), Ingley, E. (CoPI), Johnstone, L. (CoPI), Kahler, C. (CoPI), Keelan, J. (CoPI), Kular, J. (CoPI), Mullin, B. (CoPI), Nicol, M. (CoPI), Pixley, F. (CoPI), Swaminatha Iyer, I. (CoPI), Waithman, J. (CoPI), Wilson, S. (CoPI) & Woodward, E. (CoPI)
The University of Western Australia
1/01/25 → 31/12/25
Project: Research
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High throughput histopathology slide scanner
Hirvonen, L. (Investigator 01)
1/06/23 → 31/05/24
Project: Research
Equipment
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Leica TCS SP2 multiphoton confocal microscope
Centre for Microscopy, Characterisation & AnalysisFacility/equipment: Equipment
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Lionheart FX High Content Imager
Centre for Microscopy, Characterisation & AnalysisFacility/equipment: Equipment
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Courses
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SCIE5516 Materials Characterisation for Bioengineering Applications
Hirvonen, L. 1/03/21 → 31/07/22
Course