Liisa Hirvonen

Dr, BSc PhD King's College London

  • The University of Western Australia (M519), 35 Stirling Highway,

    6009 Perth


Calculated based on number of publications stored in Pure and citations from PlumX

Personal profile


I am an experimental physicist / biophysicist interested in all aspects of optical microscopy. I obtained my PhD in Physics in 2008 from King’s College London, UK, in developing the (at the time novel) Structured Illumination Microscopy (SIM) super-resolution technique. I then held several postdoctoral positions at the University of Melbourne, Australia, and King’s College London, UK, where I continued to develop novel super-resolution and time-resolved fluorescence microscopy methods, and gained extensive experience in a broad range of optical microscopy techniques (including multiphoton, TIRF, lightsheet and atomic force microscopy, various super-resolution methods and fluorescence lifetime imaging (FLIM)).

I joined the Centre for Microscopy, Characterisation and Analysis (CMCA) at UWA in 2019 as a lecturer and leader of the Optical Microscopy platform. I oversee all CMCA Optical Microscopy equipment and provide training and ongoing support to the users of the Optical Microscopy platform.


My main research interests are: 

(1) Development of novel super-resolution methods with our STORM & SIM microscopes. New fast image collection methods will enable high-speed nanoscale 3D and live cell imaging, and have applications especially in cell biology to visualise the nanoscale structure and dynamics of intracellular structures.

(2) Development and application of time-resolved fluorescence imaging (e.g. FLIM) and analysis techniques for both biological and materials sciences.

(3) Development of high resolution Correlative Light and Electron Microscopy (CLEM) workflows in collaboration with CMCA’s Electron Microscopy platform.

Teaching overview

CMCA's Optical Microscopy Training Program

SCIE5516 (Materials Characterisation for Bioengineering Applications) - Optical microscopy lectures & practicals

BMEG5001 (Advanced Topics in Biomedical Engineering) - Confocal microscopy & image processing practicals

Roles and responsibilities

Platform Leader - CMCA Optical Microscopy

WA State Rep - Light Microscopy Australia

WA State Rep - Australia and New Zealand Society for Cell and Developmental Biology (ANZSCDB)

Expertise related to UN Sustainable Development Goals

In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):

  • SDG 3 - Good Health and Well-being
  • SDG 7 - Affordable and Clean Energy
  • SDG 9 - Industry, Innovation, and Infrastructure

Education/Academic qualification

Physics, PhD, King's College London

Jul 2005Dec 2008

Award Date: 1 Dec 2008

Physics with Computer Science, BSc, King's College London

Sept 2002Jun 2005

Award Date: 30 Jun 2005

Industry keywords

  • Biotechnology
  • Bioengineering
  • Medicine and Pharma
  • Agriculture and Food
  • Oceans
  • Mining and Resources

Research expertise keywords

  • Optical Microscopy
  • Fluorescence microscopy
  • FLIM
  • Super-resolution
  • Photon counting
  • Cell imaging
  • Correlative microscopy
  • Cell biology
  • CLEM


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