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Kevin Pfleger

Professor

  • The University of Western Australia (M519), 35 Stirling Highway,

    6009 Perth

    Australia

  • 2207 Citations
  • 23 h-Index
20022022
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Research Output 2002 2019

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Article
2019
1 Citation (Scopus)

Complex Formation between VEGFR2 and the β2-Adrenoceptor

Kilpatrick, L. E., Alcobia, D. C., White, C. W., Peach, C. J., Glenn, J. R., Zimmerman, K., Kondrashov, A., Pfleger, K. D. G., Ohana, R. F., Robers, M. B., Wood, K. V., Sloan, E. K., Woolard, J. & Hill, S. J., 20 Jun 2019, In : Cell Chemical Biology. 26, 6, p. 830-841.e9

Research output: Contribution to journal › Article

Open Access
Adrenergic Receptors
Intracellular Signaling Peptides and Proteins
Bioluminescence
Endosomes
Energy Transfer

Disease-associated missense variants in ZBTB18 disrupt DNA binding and impair the development of neurons within the embryonic cerebral cortex

Hemming, I. A., Clément, O., Gladwyn-Ng, I. E., Cullen, H. D., Ng, H. L., See, H. B., Ngo, L., Ulgiati, D., Pfleger, K. D. G., Agostino, M. & Heng, J. I. T., 21 May 2019, In : Human Mutation. 15 p.

Research output: Contribution to journal › Article

Cerebral Cortex
Neurons
DNA
Zinc Fingers
Brain Diseases
3 Citations (Scopus)

NanoBRET ligand binding at a GPCR under endogenous promotion facilitated by CRISPR/Cas9 genome editing

White, C. W., Johnstone, E. K. M., See, H. B. & Pfleger, K. D. G., Feb 2019, In : Cellular Signalling. 54, p. 27-34 8 p.

Research output: Contribution to journal › Article

Transactivation of RAGE mediates angiotensin-induced inflammation and atherogenesis

Pickering, R., Tikellis, C., Rosado, C., Tsorotes, D., Dimitrropoulos, A., Smith, M., Huet, O., Seeber, R., Abhayawardana, R., Johnstone, E., Golledge, J., Wang, Y., Jandeleit-Dahm, K., Cooper, M., Pfleger, K. & Thomas, M. C., 2 Jan 2019, In : The Journal of clinical investigation. 129, 1, p. 406-421 CI99987.

Research output: Contribution to journal › Article

Angiotensins
Transcriptional Activation
Atherosclerosis
Inflammation
Angiotensin II
2018
1 Citation (Scopus)

BRET-based assay to monitor EGFR transactivation by the AT1R reveals Gq/11 protein-independent activation and AT1R-EGFR complexes

O'Brien, S. L., Johnstone, E. K. M., Devost, D., Conroy, J., Reichelt, M. E., Purdue, B. W., Ayoub, M. A., Kawai, T., Inoue, A., Eguchi, S., Hébert, T. E., Pfleger, K. D. G. & Thomas, W. G., 1 Dec 2018, In : Biochemical Pharmacology. 158, p. 232-242 11 p.

Research output: Contribution to journal › Article

Gq-G11 GTP-Binding Protein alpha Subunits
Bioluminescence
Energy Transfer
Epidermal Growth Factor Receptor
Energy transfer
1 Citation (Scopus)

Investigation of interactions between TLR2, MyD88 and TIRAP by bioluminescence resonance energy transfer is hampered by artefacts of protein overexpression

Sampaio, N. G., Kocan, M., Schofield, L., Pfleger, K. D. G. & Eriksson, E. M., 1 Aug 2018, In : PLoS One. 13, 8, e0202408.

Research output: Contribution to journal › Article

Open Access
Bioluminescence
bioluminescence
Energy Transfer
energy transfer
Artifacts
2017
13 Citations (Scopus)

Key determinants of selective binding and activation by the monocyte chemoattractant proteins at the chemokine receptor CCR2

Huma, Z. E., Sanchez, J., Lim, H. D., Bridgford, J. L., Huang, C., Parker, B. J., Pazhamalil, J. G., Porebski, B. T., Pfleger, K. D. G., Lane, J. R., Canals, M. & Stone, M. J., 23 May 2017, In : Science Signaling. 10, 480, eaai8529.

Research output: Contribution to journal › Article

Monocyte Chemoattractant Proteins
Chemokine Receptors
Chemokines
Chemical activation
Chemokine CCL2
15 Citations (Scopus)

Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time

White, C. W., Vanyai, H. K., See, H. B., Johnstone, E. K. M. & Pfleger, K. D. G., 9 Jun 2017, In : Scientific Reports. 7, 1, 3187.

Research output: Contribution to journal › Article

Open Access
Clustered Regularly Interspaced Short Palindromic Repeats
Energy Transfer
Genome
G-Protein-Coupled Receptors
Proteins
2016
39 Citations (Scopus)

Fluorescence- and bioluminescence-based approaches to study GPCR ligand binding

Stoddart, L. A., White, C., Nguyen, K., Hill, S. & Pfleger, K., 1 Oct 2016, In : British Journal of Pharmacology. 173, 20, p. 3028-3037 10 p.

Research output: Contribution to journal › Article

Open Access
Fluorescence
Ligands
Fluorescence Resonance Energy Transfer
Pharmacology
Fluorescence Polarization
95 Citations (Scopus)

G Protein-Coupled Receptor Heteromers

Gomes, I., Ayoub, M. A., Fujita, W., Jaeger, W., Pfleger, K. & Devi, L. A., 2016, In : Annual Review of Pharmacology and Toxicology. 56, p. 403-425

Research output: Contribution to journal › Article

G-Protein-Coupled Receptors
Physiological Phenomena
Pathologic Processes
Membrane Proteins
Pharmacology
23 Citations (Scopus)

i-bodies, human single domain antibodies that antagonize chemokine receptor CXCR4

Griffiths, K., Dolezal, O., Cao, B., Nilsson, S. K., See, E. B., Pfleger, K. D. G., Roche, M., Gorry, P. R., Pow, A., Viduka, K., Lim, K., Lu, B. G. C., Chang, D. H. C., Murray-Rust, T., Kvansakul, M., Perugini, M. A., Dogovski, C., Doerflinger, M., Zhang, Y., Parisi, K. & 3 othersCasey, J. L., Nuttall, S. D. & Foley, M., 2016, In : Journal of Biological Chemistry. 291, 24, p. 12641-12657 17 p.

Research output: Contribution to journal › Article

Single-Domain Antibodies
Chemokine Receptors
Stem cells
Human Body
Hematopoietic Stem Cell Mobilization
10 Citations (Scopus)

Mutations of vasopressin receptor 2 including novel L312S have differential effects on trafficking

Tiulpakov, A., White, C., Abhayawardana, R., See, H. B., Chan, A., Seeber, R., Heng, J., Dedov, I., Pavlos, N. & Pfleger, K., 1 Aug 2016, In : Molecular Endocrinology. 30, 8, p. 889-904

Research output: Contribution to journal › Article

Open Access
Vasopressin Receptors
Mutation
Hyponatremia
Nephrogenic Diabetes Insipidus
Natriuresis
2015
78 Citations (Scopus)

Application of BRET to monitor ligand binding to GPCRs

Stoddart, L. A., Johnstone, E., Wheal, A. J., Goulding, J., Robers, M. B., Machleidt, T., Wood, K. V., Hill, S. & Pfleger, K., Jul 2015, In : Nature Methods. 12, 7, p. 661-663 3 p.

Research output: Contribution to journal › Article

Open Access
Bioluminescence
Energy Transfer
G-Protein-Coupled Receptors
Energy transfer
Luminescent Proteins
19 Citations (Scopus)
291 Downloads (Pure)

Functional Interaction between Angiotensin II Receptor Type 1 and Chemokine (C-C motif) Receptor 2 with Implications for Chronic Kidney Disease

Ayoub, M., Zhang, Y., Kelly, R. S., See, E., Johnstone, E., Mccall, E. A., Williams, J. H., Kelly, D. J. & Pfleger, K., 25 Mar 2015, In : PLoS One. 10, 3, 22 p., e0119803.

Research output: Contribution to journal › Article

Open Access
File
CCR Receptors
Angiotensin Type 1 Receptor
angiotensin II
G-Protein-Coupled Receptors
kidney diseases
2014
7 Citations (Scopus)

Homogeneous time-resolved fluorescence-based assay to monitor extracellular signal-regulated kinase signaling in a high-throughput format

Ayoub, M., Trebaux, J., Vallaghe, J., Charrier-Savournin, F., Al-Hosaini, K., Gonzalez Moya, A., Pin, J-P., Pfleger, K. & Trinquet, E., 2014, In : Frontiers in Endocrinology. 5, p. 1-11

Research output: Contribution to journal › Article

Open Access
12 Citations (Scopus)

Molecular determinants of orexin receptor-arrestin-ubiquitin complex formation

Jaeger, W., Seeber, R., Eidne, K. & Pfleger, K., Jan 2014, In : British Journal of Pharmacology. 171, 2, p. 364-374

Research output: Contribution to journal › Article

Open Access
2013
15 Citations (Scopus)

AIGaN/GaN-based biosensor for label-free detection of biological activity

Podolska, A., Hool, L., Pfleger, K., Mishra, U. K., Parish, G. & Nener, B., 2013, In : SENSORS AND ACTUATORS B-CHEMICAL. 177, p. 577-582

Research output: Contribution to journal › Article

19 Citations (Scopus)

Characterization of Three Vasopressin Receptor 2 Variants: An Apparent Polymorphism (V266A) and Two Loss-of-Function Mutations (R181C and M311V)

Armstrong, S., Seeber, R., Ayoub, M., Feldman, B. J. & Pfleger, K., 2013, In : PLoS One. 8, 6, p. 9pp e65885.

Research output: Contribution to journal › Article

Open Access
31 Citations (Scopus)

Identification and profiling of CXCR3-CXCR4 chemokine receptor heteromer complexes

Watts, A. O., Van Lipzig, M. M. H., Jaeger, W., Seeber, R., Van Zwam, M., Vinet, J., Van Der Lee, M. M. C., Siderius, M. H., Zaman, G. J. R., Boddeke, H. W. G. M., Smit, M. J., Pfleger, K., Leurs, R. & Vischer, H. F., 2013, In : British Journal of Pharmacology. 168, 7, p. 1662-1674

Research output: Contribution to journal › Article

CXC Chemokines
Chemokine Receptors
CC Chemokines
Chemokines
CCR2 Receptors
9 Citations (Scopus)

Profiling Epidermal Growth Factor Receptor and Heregulin Receptor 3 Heteromerization Using Receptor Tyrosine Kinase Heteromer Investigation Technology

Ayoub, M., See, E., Seeber, R., Armstrong, S. & Pfleger, K., 2013, In : PLoS One. 8, 5, p. 10pp e64672.

Research output: Contribution to journal › Article

Open Access
19 Citations (Scopus)

Quantitative Förster resonance energy transfer efficiency measurements using simultaneous spectral unmixing of excitation and emission spectra

Mustafa, S., Hannagan, J., Rigby, P., Pfleger, K. & Corry, B., 2013, In : Journal of Biomedical Optics. 18, 2, p. 026024-1 - 026024-14

Research output: Contribution to journal › Article

Open Access
2012
17 Citations (Scopus)

Biocompatibility of semiconducting AIGaN/GaN material with living cells

Podolska, A., Tham, S., Hart, R. D., Seeber, R., Kocan, M., Kocan, M., Mishra, U. K., Pfleger, K., Parish, G. & Nener, B., 2012, In : SENSORS AND ACTUATORS B-CHEMICAL. 169, p. 401-406

Research output: Contribution to journal › Article

29 Citations (Scopus)

Deleterious GRM1 Mutations in Schizophrenia

Ayoub, M., Angelicheva, D., Vile, D., Chandler, D., Morar, B., Cavanaugh, J. A., Visscher, P. M., Jablensky, A., Pfleger, K. & Kalaydjieva, L., 2012, In : PLoS One. 7, p. 8pp e32849.

Research output: Contribution to journal › Article

Open Access
35 Citations (Scopus)

Effect of enhanced Renilla luciferase and fluorescent protein variants on the Förster distance of Bioluminescence resonance energy transfer (BRET)

Dacres, H., Michie, M., Wang, J., Pfleger, K. & Trowell, S. C., 2012, In : Biochemical and Biophysical Research Communications. 425, p. 625-629

Research output: Contribution to journal › Article

36 Citations (Scopus)

Identification and profiling of novel α 1A-adrenoceptor-CXC chemokine receptor 2 heteromer

Mustafa, S., See, E., Seeber, R., Armstrong, S. P., White, C. W., Ventura, S., Ayoub, M. & Pfleger, K., 2012, In : Journal of Biological Chemistry. 287, 16, p. 12952-12965

Research output: Contribution to journal › Article

Open Access
2011
39 Citations (Scopus)

Application of G Protein-Coupled Receptor-Heteromer Identification Technology to Monitor beta-arrestin Recruitment to G Protein-Coupled Receptor Heteromers

See, E., Seeber, R., Kocan, M., Eidne, K. & Pfleger, K., 2011, In : Assay and Drug Development Technologies. 9, 1, p. 21-30

Research output: Contribution to journal › Article

Enhanced BRET technology for the monitoring of agonist-induced and agonist-independent interactions between GPCRs and β-arrestins

Kocan, M., Dalrymple, M., Seeber, R., Feldman, B. J. & Pfleger, K., 2011, In : Frontiers in Endocrinology. 1, p. 1-9 12.

Research output: Contribution to journal › Article

14 Citations (Scopus)

G Protein-Coupled Receptor Heteromer Identification Technology: Identification and Profiling of GPCR Heteromers

Mustafa, S. & Pfleger, K., 2011, In : Journal of Laboratory Automation. 16, p. 285-291

Research output: Contribution to journal › Article

Open Access
35 Citations (Scopus)

Heteromerization of angiotensin receptors changes trafficking and arrestin recruitment profiles

Porrello, E. R., Pfleger, K., Seeber, R., Qian, H., Oro, C., Abogadie, F., Delbridge, L. M. D. & Thomas, W. G., 2011, In : Cellular Signalling. 23, p. 1767-1776

Research output: Contribution to journal › Article

20 Citations (Scopus)

Temporal Profiling of Orexin Receptor-Arrestin-Ubiquitin Complexes Reveals Differences between Receptor Subtypes

Dalrymple, M., Jaeger, W., Eidne, K. & Pfleger, K., 2011, In : Journal of Biological Chemistry. 286, p. 16726-16733

Research output: Contribution to journal › Article

Open Access
2010
47 Citations (Scopus)

Differential association modes of the thrombin receptor PAR1 with Gαi1, Gα12, and β-arrestin 1

Ayoub, M., Trinquet, E., Pfleger, K. & Pin, J-P., 2010, In : FASEB Journal. 24, p. 3522-3535

Research output: Contribution to journal › Article

73 Citations (Scopus)

Recent advances in bioluminescence resonance energy transfer technologies to study GPCR heteromerization

Ayoub, M. & Pfleger, K., 2010, In : Current Opinion in Pharmacology. 10, p. 44-52

Research output: Contribution to journal › Article

14 Citations (Scopus)

Uncovering GPCR heteromer-biased ligands

Mustafa, S., Ayoub, M. & Pfleger, K., 2010, In : Drug Discovery Today. 7, p. 77-85

Research output: Contribution to journal › Article

2009
33 Citations (Scopus)

Agonist-independent interactions between beta-arrestins and mutant vasopressin type II receptors associated with nephrogenic syndrome of inappropriate antidiuresis

Kocan, M., See, E., Sampaio, N., Eidne, K., Feldman, B. J. & Pfleger, K., 2009, In : Molecular Endocrinology. 23, 4, p. 559-571

Research output: Contribution to journal › Article

Vasopressin Receptors
Hyponatremia
Codon
Mutation
Arginine
256 Citations (Scopus)

Building a new conceptual framework for receptor heteromers

Ferré, S., Baler, R., Bouvier, M., Caron, M. G., Devi, L. A., Fuxw, K., Durroux, T., Javitch, J. A., George, S. R., Lohse, M. J., Mackie, K., Milligan, G., Pfleger, K., Pin, J. P., Volkow, N. D., Waldhoer, M., Woods, A. S. & Franco, R., 2009, In : Nature Chemical Biology. 5, 3, p. 131-134

Research output: Contribution to journal › Article

G-protein-coupled receptors in drug discovery - seventh annual Informa conference

Pfleger, K., 2009, In : IDRUGS. 12, 5, p. 288-290

Research output: Contribution to journal › Article

2008
20 Citations (Scopus)

Changes to Gonadotropin-Releasing Hormone (GnRH) Receptor Extracellular Loops Differtially Affect GnRH Analog Binding and Activation : Evidence for Distinct Ligand-Stabilized Receptor Conformations

Pfleger, K., Pawson, A. J. & Millar, R. P., 2008, In : Endocrinology. 149, 6, p. 3118-3129

Research output: Contribution to journal › Article

LHRH Receptors
Gonadotropin-Releasing Hormone
Ligands
Catfishes
Mutation
70 Citations (Scopus)

Demonstration of Improvements to the Bioluminescence Resonance Energy Transfer (BRET) Technology for the Monitoring of G Protein-Coupled Receptors in Live Cells

Kocan, M., See, E., Seeber, R., Eidne, K. & Pfleger, K., 2008, In : Journal of Biomolecular Screening. 13, 9, p. 888-898

Research output: Contribution to journal › Article

Bioluminescence
Arrestin
Energy Transfer
G-Protein-Coupled Receptors
Thyrotropin Releasing Hormone Receptors
81 Citations (Scopus)

G protein-coupled receptor dimers: Functional consequences, disease states and drug targets

Dalrymple, M., Pfleger, K. & Eidne, K., 2008, In : Pharmacology & Therapeutics. 118, 3, p. 359-371

Research output: Contribution to journal › Article

G-Protein-Coupled Receptors
Pharmacology
Pharmaceutical Preparations
Ligands
Therapeutics

G Protein Coupled Receptors as Drug Targets: The Role of scientific symbols beta-Arrestins

Dromey, J. & Pfleger, K., 2008, In : Endocrine, Metabolic & Immune Disorders - Drug Targets. 7, p. 51-61

Research output: Contribution to journal › Article

2007
30 Citations (Scopus)

Monitoring interactions between G-protein-coupled receptors and ß-arrestins

Pfleger, K., Dalrymple, M., Dromey, J. & Eidne, K., 2007, In : Biochemical Society Transactions. 35, 4, p. 764-766

Research output: Contribution to journal › Article

Arrestins
G-Protein-Coupled Receptors
Monitoring
Bioluminescence
Arrestin
2006
128 Citations (Scopus)

Bioluminescence resonance energy transfer (BRET) for the real-time detection of protein-protein interactions

Pfleger, K., Seeber, R. & Eidne, K., 2006, In : Nature Protocols. 1, 1, p. 336-345

Research output: Contribution to journal › Article

Bioluminescence
Energy Transfer
Energy transfer
Proteins
Cell culture
77 Citations (Scopus)

Extended bioluminescence resonance energy transfer (eBRET) for monitoring prolonged protein-protein interactions in live cells

Pfleger, K., Dromey, J., Dalrymple, M., Lim, E., Thomas, W. G. & Eidne, K., 2006, In : Cellular Signalling. 18, 10, p. 1664-1670

Research output: Contribution to journal › Article

Energy Transfer
Proteins
Drug Industry
G-Protein-Coupled Receptors
Technology
354 Citations (Scopus)

Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET)

Pfleger, K. & Eidne, K., 2006, In : Nature Methods. 3, 3, p. 165-174

Research output: Contribution to journal › Article

Bioluminescence
Energy Transfer
Energy transfer
Proteins
Molecules
2005
9 Citations (Scopus)

Helix I of beta-arrestin is involved in postendocytic trafficking but is not required for membrane translocation, receptor binding, and internalization

Dinh, D. T., Qian, H., Seeber, R., Lim, E., Pfleger, K., Eidne, K. & Thomas, W. G., 2005, In : Molecular Pharmacology. 67, 2, p. 375-382

Research output: Contribution to journal › Article

Membranes
Transport Vesicles
Angiotensin Type 1 Receptor
beta-Arrestins
Energy Transfer
2004
23 Citations (Scopus)

Gonadotropin-Releasing Hormone Receptor-Mediated Growth Suppression of Immortalized LβT2 Gonadotrope and Stable HEK293 Cell Lines

Miles, L. E. C., Hanyaloglu, A. C., Dromey, J., Pfleger, K. & Eidne, K., 2004, In : Endocrinology. 145, 1, p. 194-204

Research output: Contribution to journal › Article

LHRH Receptors
HEK293 Cells
Gonadotropin-Releasing Hormone
Cell Line
Growth
2003
75 Citations (Scopus)

G-protein coupled receptor oligomerization in neuroendocrine pathways

Kroeger, K. M., Pfleger, K. & Eidne, K., 2003, In : Frontiers in Neuroendocrinology. 24, 4, p. 254-278

Research output: Contribution to journal › Article

G-Protein-Coupled Receptors
Biological Phenomena
Drug Design
Dimerization
Pharmacology
36 Citations (Scopus)

New Technologies: Bioluminescence Resonance Energy Transfer (BRET) for the Detection of Real Time Interactions Involving G-Protein Coupled Receptors

Pfleger, K. & Eidne, K. A., 2003, In : Pituitary. 6, 3, p. 141-151

Research output: Contribution to journal › Article

2002
31 Citations (Scopus)

Conformational Constraint of Mammalian, Chicken, and Salmon GnRHs, But Not GnRH II, Enhances Binding at Mammalian and Nonmammalian Receptors: Evidence for Preconfiguration of GnRH II

Pfleger, K., Bogerd, J. & Millar, R. P., 2002, In : Molecular Endocrinology. 16, p. 2155-2162

Research output: Contribution to journal › Article